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Roselle fermentation product and preparation method and application thereof

The technology of a fermentation product and roselle is applied in the field of roselle fermentation product and its preparation, which can solve the problems of strong irritation and poor whitening effect, and achieves enhancement of skin elasticity, good inhibition of tyrosinase activity, and reduction of irritation. Effect

Active Publication Date: 2021-12-28
GUANGZHOU HUANYA COSMETIC SCI & TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the application of roselle fermented products in cosmetics has some problems such as poor whitening effect and strong irritation

Method used

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  • Roselle fermentation product and preparation method and application thereof
  • Roselle fermentation product and preparation method and application thereof
  • Roselle fermentation product and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] A preparation method of roselle flower fermentation product, comprising the following steps:

[0034] (1) take by weighing 100g roselle dried flower and pulverize, sieve 100 orders, obtain roselle powder, the volume concentration that adds roselle powder to 1000g is in the ethanol solution of 50% (the mass ratio of powder and solvent is 1:10), under the pressure of 20Mpa, carry out high-pressure homogeneous extraction for 30 minutes, membrane filtration, and then concentrate under reduced pressure at 60°C to remove ethanol, and continue to concentrate to 100mL to obtain roselle flower extract;

[0035] (2) Mix 20mL roselle flower extract, 2mL mixed bacterial solution (1mL Saccharomyces cerevisiae and 1mL Lactococcus lactis, the concentration of the bacterial solution is 10 7 CFU / mL), 2g MRS broth medium and 80mL deionized water, adjust the pH to 5.0, and ferment on a shaker at 30°C with a rotation speed of 180rpm / min, and the fermentation time is 96h. Sterilize at ℃ fo...

Embodiment 2

[0037] A preparation method of roselle flower fermentation product, comprising the following steps:

[0038] (1) take by weighing 100g roselle dried flower and pulverize, sieve 150 orders, obtain roselle powder, the volume concentration that adds roselle powder to 500g is in the ethanol solution of 70% (the mass ratio of powder and solvent is 1:5), under the pressure of 10Mpa, carry out high-pressure homogeneous extraction for 30 minutes, membrane filtration, and then concentrate under reduced pressure at 60°C to remove ethanol, and continue to concentrate to 100mL to obtain roselle flower extract;

[0039] (2) Mix 100mL roselle flower extract, 5mL mixed bacterial solution (2.5mL S. 5 CFU / mL), 5g MRS broth medium, and 400mL deionized water were mixed, adjusted to pH 6.0, and fermented on a shaker at 35°C with a rotation speed of 200rpm / min and a fermentation time of 36h. Sterilize at ℃ for 30 minutes, then centrifuge at a centrifugal radius of 11 cm and a rotating speed of 30...

Embodiment 3

[0041] A preparation method of roselle flower fermentation product, comprising the following steps:

[0042] (1) take by weighing 100g roselle dried flower and pulverize, sieve 100 orders, obtain roselle powder, the volume concentration that adds roselle powder to 1000g is in the ethanol solution of 50% (the mass ratio of powder and solvent is 1:10), under the pressure of 30Mpa, carry out high-pressure homogeneous extraction for 10min, membrane filtration, and then concentrate under reduced pressure at 60°C to remove ethanol, and continue to concentrate to 100mL to obtain roselle flower extract;

[0043] (2) Mix 10mL roselle flower extract, 1mL mixed bacterial solution (0.5mL Kluyveromyces lactis and 0.5mL Lactobacillus plantarum, the concentration of the bacterial solution is 10 6 CFU / mL), 1g MRS broth medium, and 5mL deionized water were mixed, adjusted to pH 4.0, and fermented on a shaker at 28°C with a rotation speed of 160rpm / min and a fermentation time of 120h. Steriliz...

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Abstract

The invention belongs to the technical field of cosmetics, and discloses a roselle fermentation product and a preparation method and application thereof. The preparation method of the roselle fermentation product comprises the following steps of 1) extraction, specifically, mixing roselle with a solvent, and performing high-pressure homogeneous extraction under the pressure intensity of 10-30 Mpa to obtain a roselle extracting solution; and 2) fermentation, specifically, adding a culture medium into the roselle extracting solution, and then inoculating a mixed bacteria solution for a fermentation reaction to obtain the roselle fermentation product, wherein the mixed bacteria solution comprises saccharomycetes and lactic acid bacteria. By means of the preparation method, roselle flowers are subjected to high-pressure homogeneous extraction, so that the extraction rate of effective components of the roselle flowers is increased; meanwhile, the saccharomycetes and the lactic acid bacteria are compounded for fermentation, and the roselle flower fermentation product not only can retain the effective components of the roselle flowers, but also contains new secondary metabolites generated by microorganisms, so that effective active components can be increased, toxic and side effects possibly generated by the roselle flowers are reduced, and the efficacy of the roselle flower fermentation product is improved.

Description

technical field [0001] The invention belongs to the technical field of cosmetics, in particular to a roselle fermented product and its preparation method and application. Background technique [0002] Roselle (scientific name: Hibiscus sabdariffaLinn.) is a plant of the genus Hibiscus in the Malvaceae family, also known as Roselle, Luohuashen, Burning Flower God, and Burning God Flower. It is native to the tropical regions of the eastern hemisphere and is cultivated in tropical regions of the world, as well as in Taiwan, Fujian, Guangdong and southern Yunnan. Roselle flowers are purple-red, bright in color, and the corolla and flower statues are beautiful and unique. It is an excellent ornamental plant for beautifying the environment. Roselle is a medicinal and edible plant, which is widely used to make tea, beverages, fruit juices and other bulk consumer products. Modern dermatological research shows that roselle flower ingredients are rich in protein, polysaccharides, or...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K8/9789A61Q19/02A61Q19/08
CPCA61K8/9789A61Q19/02A61Q19/08A61K2800/782A61K2800/85
Inventor 戴仟福何敬愉陈慧芳陈英杰潘丹阳
Owner GUANGZHOU HUANYA COSMETIC SCI & TECH CO LTD
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