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Tuna roe dipeptidyl peptidase IV (DPP-IV) inhibition oligopeptide

A dipeptidyl peptidase and tuna technology, applied in the field of peptides, can solve problems such as waste of tuna resources, and achieve the effects of safety, no toxic side effects, content reduction, and blood sugar level reduction.

Active Publication Date: 2021-12-28
ZHEJIANG OCEAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

During the tuna processing process, about 50% to 70% of the total weight of by-products are produced. These by-products are mainly tuna viscera, minced meat, fish head, fish skin and fish bones, etc., which are used as feed raw materials or primary feed in large quantities, resulting in This not only caused a great waste of tuna resources, but also brought great pressure to the ecological environment.

Method used

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  • Tuna roe dipeptidyl peptidase IV (DPP-IV) inhibition oligopeptide
  • Tuna roe dipeptidyl peptidase IV (DPP-IV) inhibition oligopeptide
  • Tuna roe dipeptidyl peptidase IV (DPP-IV) inhibition oligopeptide

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Experimental program
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Embodiment

[0036] 1) Pretreatment of tuna roe: thaw skipjack roe, remove impurities, weigh 1000g, mash with a tissue grinder, add acetone solution according to the ratio of material to liquid 1g:8mL, degrease with 200W ultrasound for 20min, repeat three times, Centrifuge at 4°C, 9000rmp for 15min, and dry the solid precipitate to obtain defatted fish eggs;

[0037] 2) Enzymatic hydrolysis of fish roe: Add the above-mentioned defatted fish roe into pH 2.0 buffer solution according to the ratio of material to liquid 1g:12mL, stir well, adjust the temperature of the solution to 37°C, add 2.5% pepsin (1.5× 10 4 U / g), enzymolysis for 3.5h; then adjust the pH of the solution to 7.0, add 2.0% trypsin (2.0×10 4U / g), enzymatic hydrolysis for 4 hours; after the enzymatic hydrolysis, the solution was cooled to room temperature, centrifuged at 12000rmp for 10-15min, and the supernatant was collected, that is, the enzymatic hydrolyzate of fish egg protein;

[0038] 3) Preparation of fish egg dipept...

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Abstract

The invention discloses tuna roe dipeptidyl peptidase IV (DPP-IV) inhibition oligopeptide and application thereof. The amino acid sequence of the DPP-IV inhibition oligopeptide is Glu-Ile-Pro-Gly-Thr-Arg-Gly-Pro-Leu, and the molecular weight of the DPP-IV inhibition oligopeptide is 939.07 Da. The prepared tuna roe DPP-IV inhibition oligopeptide can significantly inhibit the activity of DPP-IV, reduce the postprandial blood sugar level of a model mouse, improve the oral glucose tolerance and maltose tolerance of the mouse and reduce the content of triglyceride (TG) and cholesterol (TC) of the model mouse, and is safe and free of toxic and side effects; and the tuna roe DPP-IV inhibition oligopeptide can be applied to preparation of food, health-care products and medicines with special medical purposes for treatment or adjuvant treatment of diabetes mellitus type 2 (T2DM).

Description

technical field [0001] The invention relates to the technical field of polypeptides, in particular to an inhibitory oligopeptide derived from dipeptidyl peptidase IV (DPP-IV) derived from tuna roe and used for treating type II diabetes. Background technique [0002] Diabetes is a metabolic disease characterized by high blood sugar. Hyperglycemia is caused by defective insulin secretion or impaired biological action, or both. Type II diabetes (diabetes mellitus type 2, T2DM), formerly known as non-insulin-dependent diabetes or adult-onset diabetes, is a chronic metabolic disease that usually occurs after the age of 35-40. According to the latest data released by the National Health and Family Planning Commission, the number of people with diabetes in my country has reached 114 million, and more than 90% of them are type II diabetes patients. [0003] Glucagon-like peptide 1 (GLP-1) is a brain-gut peptide composed of 30 amino acids secreted by intestinal L cells, which has b...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K7/06C12N9/48C12P21/06C07K1/34C07K1/16C07K1/20C07K1/36A61K38/08A61P3/10A61P3/06A23L33/18
CPCC07K7/06C12N9/485C12P21/06A61P3/10A61P3/06A23L33/18C12Y304/14005A61K38/00A23V2002/00A23V2200/328A23V2200/3262A23V2250/55
Inventor 陈如萍王玉梅王斌
Owner ZHEJIANG OCEAN UNIV
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