A kind of extraction method and application of sweet tea polyphenol

Abstract

The invention discloses a method for extracting sweet tea polyphenols and its application. The extraction method comprises: taking the stock solution of sweet tea polyphenols obtained after extracting rubusoside from sweet tea as a raw material, and performing two purifications with a macroporous resin column after adjusting the pH. The water eluate collected in the first purification is subjected to the second purification, and then the ethanol eluate collected in the two purifications is combined, de-alcoholized and diluted, then filtered, put on a polyamide resin column and eluted with water and ethanol solution successively , collect the ethanol eluate and post-process. The present invention adopts the macroporous resin column to carry out two purifications, which can greatly improve its recovery rate, and is simple to operate and low in cost; eluting and analyzing with ethanol solution with pH 8-9 can reduce the adsorption of sweet tea polyphenols on the polyamide resin, and improve Recovery rate and product purity; before passing through the polyamide resin column, filter with microporous membrane and ultrafiltration membrane successively, so that subsequent separation and purification are more convenient; the method of the invention can effectively realize the full utilization of resources, and has broad application prospects.

Description

technical field [0001] The invention relates to the technical field of extraction of plant active ingredients, and more particularly, to a method for extracting sweet tea polyphenols and applications thereof. Background technique [0002] Guangxi sweet tea (Rubus Suavissimus S. Lee) belongs to the genus Rosaceae, and its main active ingredients include sweet tea glycosides, sweet tea polyphenols and sweet tea flavonoids. [0003] Rubaside is the main component of sweet tea, specifically a terpenoid component. Because it has no calories and is 300 times sweeter than sucrose, it is favored by sub-healthy people, especially obese people. As a food additive, it is widely used, and therefore, sweet tea glucoside extract has also become a popular product in the industry. [0004] Most of the sweet tea polyphenols belong to condensed tannins, also known as tannins, which have good water solubility. The content of sweet tea polyphenols in the dried sweet tea leaves is as high as 18...

AI Technical Summary

Problems solved by technology

[0005] At present, there are many related reports on the extraction and purification of rubusoside and sweet tea polyphenols in sweet tea; in the prior art, the extraction process of sweet tea polyphenols is mostly to wash the effluent of the macroporous resin column with low-concentration ethanol. The preliminary preparation of sweet tea polyphenols is carried out by dehydration and combination. In the subsequent further purification, repeated extraction and gel resin enrichment are used, and organic reagents such as acetone are used. The steps are cumbersome in the scale-up production, and the use of organic reagents is limited. , not suitable for industrial production

[0006] The Chinese invention patent application with the application number CN201510700707.8 discloses a process for the simultaneous preparation of rubusoside and sweet tea total polyphenols. The sweet tea powder is extracted with water, the extract is passed through a macroporous resin column, and the effluent and low-concentration ethanol are collected. The eluent is combined and the two are passed through the macroporous resin column again, and the 40% ethanol eluent is collected again, and the obtained polyphenol purity is greater than or equal to 60%, that is, the polyphenol purity obtained by this process is low, and the yield is lower Low (about 10%)

[0007] The Chinese invention patent application with the application number CN201810688181.X discloses a method for extracting theanine from sweet tea and simultaneously extracting rubusoside and tea polyphenols. The method specifically includes collecting the effluent from a macroporous resin column and using polyamide The resin is enriched and purified, collected and dried with high concentration ethanol, but the recovery rate is unknown