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Method for detecting systemic pesticide dimethoate residue

A systemic and pesticide technology, applied in the field of detection of systemic pesticide dimethoate residues, can solve the problems of high requirements on instrument conditions, low detection limit sensitivity, and limited on-site detection, etc., achieve low requirements on instrument conditions and improve detection sensitivity , low cost effect

Active Publication Date: 2022-01-04
广东江门中医药职业学院
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

But most of the existing detection methods for dimethoate residues are gas chromatography, high performance liquid chromatography, and liquid chromatography-mass spectrometry. Although these methods have the advantages of low detection limit and high sensitivity, there are still some problems, such as consumption Long time, high cost, and high requirements for instrument conditions limit the application of on-site testing

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  • Method for detecting systemic pesticide dimethoate residue

Examples

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Effect test

Embodiment 1

[0035] In this embodiment, petroleum ether is selected from analytically pure petroleum ether supplied by Sinopharm Chemical Reagent Co., Ltd., diethyl ether is selected from analytically pure diethyl ether supplied from Sinopharm Chemical Reagent Co., Ltd., and disodium hydrogen phosphate is selected from analytically pure hydrogen phosphate supplied from Sinopharm Chemical Reagent Co., Ltd. Disodium, citric acid selects 99% citric acid supplied by Sinopharm Chemical Reagent Co., Ltd., chloroform selects analytical pure chloroform supplied by Sinopharm Chemical Reagent Co., Ltd., and 33% diethylamine aqueous solution selects Sinopharm Chemical Reagent Co., Ltd. to supply Analytical pure 33% diethylamine aqueous solution, hydrogen peroxide chooses analytical pure 30% hydrogen peroxide supplied by Sinopharm Chemical Reagent Co., Ltd., and anhydrous dipotassium hydrogen phosphate chooses analytical pure anhydrous phosphoric acid supplied by Sinopharm Chemical Reagent Co., Ltd. Di...

Embodiment 2

[0044] A method for detecting systemic pesticide dimethoate residues of the present invention comprises the following:

[0045] Step a, weighing 100g of the vegetable sample to be tested in a homogenizer, pulverizing to obtain the first sample, for use;

[0046] Step b, weigh 5g of the first sample into a 50ml first centrifuge tube, add 10ml of pH 6.0 sodium salt solution, fully shake and mix for 2 minutes, add 5ml of chloroform, shake and mix for 2 minutes, then put it in the centrifuge , centrifuge at 10000rpm for 3min until the solution in the first centrifuge tube is clearly stratified, take 4.5ml of the lower chloroform phase solution into the 15ml second centrifuge tube to obtain the first sample solution, the sodium salt solution of pH6.0 is disodium hydrogen phosphate solution and the mixed solution of citric acid solution;

[0047] Step c, put the second centrifuge tube into the air concentrator, and dry the first sample liquid with air at 65°C to obtain the first an...

Embodiment 3

[0053] A method for detecting systemic pesticide dimethoate residues of the present invention comprises the following:

[0054] Step a, weighing 100g of the fruit sample to be tested in a homogenizer, pulverizing to obtain the first sample, for use;

[0055] Step b, weigh 5g of the first sample into a 50ml first centrifuge tube, add 10ml of pH 6.0 sodium salt solution, fully shake and mix for 2 minutes, add 5ml of chloroform, shake and mix for 2 minutes, then put it in the centrifuge , centrifuge at 10000rpm for 3min until the solution in the first centrifuge tube is clearly stratified, take 4.5ml of the lower chloroform phase solution into the 15ml second centrifuge tube to obtain the first sample solution, the sodium salt solution of pH6.0 is disodium hydrogen phosphate solution and the mixed solution of citric acid solution;

[0056] Step c, put the second centrifuge tube into the air concentrator, and dry the first sample liquid with air at 65°C to obtain the first analyt...

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Abstract

The invention discloses a method for detecting systemic pesticide dimethoate residue. The method comprises the following steps: step a, crushing a sample to be detected to obtain a first sample; step b, adding a sodium salt solution and chloroform into the first sample, performing centrifugal treatment until the solution is obviously layered, and taking a chloroform phase solution to obtain a first sample solution; step c, blow-drying the first sample liquid to obtain a first substance to be detected; step d, adding diethylamine into the first substance to be detected, redissolving, adding an oxidizing agent, adjusting the temperature to 80 DEG C, standing to obtain a second sample solution, and blow-drying to obtain a second substance to be detected; step e, adding an ether organic solvent into the second substance to be detected, redissolving, slowly adding a buffer solution, adding an acetylcholin esterase reagent, and standing; step f, adding a substrate and a color developing agent, standing for 3 minutes, observing the color change of a lower layer after the solution is layered, shaking, standing, and observing the color change of the lower layer after the solution is layered; and step g, judging a result.

Description

technical field [0001] The invention relates to the field of food safety detection, in particular to a method for detecting residues of systemic pesticide dimethoate. Background technique [0002] Dimethoate is widely used in fruits, vegetables, cotton and other crops because of its good effect of rapid insecticide and acaricide. It is one of the most widely used systemic pesticides. However, while increasing the yield of crops, the excessive use of dimethoate has caused a large amount of pesticide residues in fruits and oil crops, which has caused serious harm to human health. Dimethoate can enter human tissue through skin contact and other channels, causing acute or chronic poisoning, and even death in severe cases. [0003] Dimethoate is prohibited from being used on vegetables, fruits, tea, fungi and Chinese herbal medicine crops. Therefore, the detection of dimethoate residues in agricultural products is of great significance. But most of the existing detection metho...

Claims

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Application Information

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IPC IPC(8): G01N21/78G01N33/02G01N1/28G01N1/38
CPCG01N21/78G01N33/02G01N1/286G01N1/38
Inventor 李拥军熊文明皮小弟廖雪义李叶青汤胤旻姚鸿州周敏瑜
Owner 广东江门中医药职业学院
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