Rapid heating or cooling temperature control method applied to nucleic acid amplification technology

Abstract

The invention relates to the technical field of temperature control methods, in particular to a rapid heating or cooling temperature control method applied to a nucleic acid amplification technology. The rapid heating or cooling temperature control method comprises the following steps of controlling a rotating motor to operate to drive a container containing amplification liquid to rotate, and after hot or cold air at a certain temperature is accelerated through a nozzle, rapidly heating or cooling the amplification liquid in a jet heat exchange mode. The amplification liquid in the container is heated or cooled in an air injection mode under a rotating condition, and multiple times of temperature circulation of the amplification liquid under the conditions of a given temperature level, maintenance time and the like are achieved. The gas injection heat exchange mode is based on high-speed forced convection heat exchange, rapid heat exchange between air and the amplification liquid is facilitated, and rapid control over the heating and cooling rate of the amplification liquid is achieved. Under the rotating condition, a secondary flow formed in the amplification liquid can effectively mix the amplification liquid in different areas in the container, so that the temperature uniformity of the amplification liquid in the container is remarkably improved.

Description

technical field [0001] The invention relates to the technical field of temperature control methods, in particular to a temperature control method for rapid heating or cooling applied to nucleic acid amplification technology. Background technique [0002] At present, nucleic acid amplification biotechnology is developing rapidly, and nucleic acid amplification technology based on PCR (polymerase chain reaction) tubes, microfluidic chips and other devices has a good development prospect. Nucleic acid amplification technology usually needs to realize rapid switching between several temperature points for the temperature control target. The temperature change in the traditional PCR process is as follows: figure 1 Shown: Switch between 40°C-60°C, 70°C-75°C, 90°C-95°C and complete 20-30 cycles; the speed of temperature switching greatly affects the PCR (polymerase chain reaction) reaction The efficiency of the reaction will affect the quality of the final detection result, so it ...

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Problems solved by technology

[0003] Most of the existing temperature control methods based on nucleic acid amplification technology have shortcomings such as low heating and cooling rates and uneven temperature of the metal substrate.

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