Calibration device for fluorescence immunoassay quantitative analyzer

Abstract

The invention discloses a calibration device for a fluorescence immunoassay quantitative analyzer, which relates to the technical field of fluorescence immunoassay quantitative analyzers. A first half-reflecting mirror is arranged at a main optical axis between a first convex lens and a dichroic mirror; a total-reflecting mirror is arranged at the main optical axis of the reflected light of the first half-reflecting mirror; a second half-reflecting mirror is arranged at the main optical axis of the reflected light of the total-reflecting mirror; and a fourth convex lens and a second cylindrical mirror are arranged on the reflecting side of the second half-reflecting mirror. Incident light is shunted to the back of the sample wafer through the first half-reflecting mirror, the total-reflecting mirror and the second half-reflecting mirror, so that the data of the front and back surfaces of the same sample wafer under the same light source, time, position and environment are synchronously measured, and the fluorescence data measured on the front surface is subtracted from the reference data on the back surface to obtain calibrated fluorescence data. The device has the outstanding characteristics that fluctuation and errors caused by particularity and contingency of materials, abrasion of a light source, abrasion of a mechanical transmission structure and the like can be removed.

Description

technical field [0001] The invention relates to the technical field of fluorescent immunoassay analyzers, in particular to a calibration device for fluorescent immunoassays. Background technique [0002] In the use of the fluorescence immunoassay analyzer, due to the loss of the light source, the wear of the mechanical transmission structure, and the change of the ambient temperature and humidity, the calibration method is not uniform, etc., it is easy to cause the error of the fluorescence immunoassay analysis, which seriously affects the accuracy of multiple measurements. Accuracy and repeatability. In the prior art, a blank card is usually used as a calibration card, but the calibration card and the measurement card may not achieve a more accurate calibration goal due to different batches, humidity, and processes, and even if the two are Cards under the same batch, humidity, and process are also difficult to overcome fluctuations and errors caused by card particularity an...

AI Technical Summary

Problems solved by technology

[0002] During the use of the fluorescent immunoassay analyzer, due to the loss of the light source, the wear of the mechanical transmission structure, and the change of the ambient temperature and humidity, the calibration method is not uniform, etc. It is easy to cause mistakes in fluorescent immunoquantitative analysis, which seriously affects the accuracy and repeatability of multiple measurements. In the prior art, blank cards are usually used as calibration cards, but calibration cards and measurement cards may be due to different batches, humidity, and processes. However, a more accurate calibration purpose cannot be achieved, and even if the two cards are of the same batch, humidity, and process, it is difficult to overcome the fluctuations and errors caused by the particularity and chance of the card, so a more accurate calibration device is needed. Guarantees the accuracy and repeatability of multiple measurements

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