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Multi-stage inertial micro-fluidic blood sample processing chip integrating micro-mixer and Tesla valve

A micro-mixer and processing chip technology, which is applied in the direction of mixers, laboratory appliances, and laboratory containers, can solve the problems of low sample throughput, low processing efficiency, and low precision, and achieve the effect of high screening purity

Active Publication Date: 2022-01-21
ZHONGBEI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The large-scale clinical application of this detection scheme using microfluidic technology faces a difficulty, that is, it is difficult to have both the manipulation accuracy and processing efficiency of the cells by the microfluidic unit. High, but low sample throughput and low processing efficiencies (e.g. Figure 7 shown); passive microfluidics that use fluid drag to manipulate based on cell size differences have high sample throughput (eg Figure 8 shown), can achieve high processing efficiency, but the accuracy is low, and non-target cells often mix with target cells

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  • Multi-stage inertial micro-fluidic blood sample processing chip integrating micro-mixer and Tesla valve
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  • Multi-stage inertial micro-fluidic blood sample processing chip integrating micro-mixer and Tesla valve

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Embodiment 1

[0023] Such as figure 1 , 2 As shown, a multi-stage inertial microfluidic blood sample processing chip integrating a micro-mixer and a Tesla valve, the chip is prepared with an inlet area, a micro-mixer, a first-stage inertial sorting unit, a Tesla valve flow resistance matching area, A secondary inertial sorting unit and an outlet area; the inlet area is composed of a trace blood sample inlet and a buffer inlet and an intermediate section connecting the trace blood sample inlet and the buffer inlet, and the intermediate section is connected to a micro-mixer; the micro-mixer It consists of a plurality of sequentially connected micro-mixing units and final channels, each micro-mixing unit consists of a first channel, a second channel connected to the first channel at an obtuse angle, a third channel connected to the second channel at a right angle, and It is composed of a fourth channel at right angles to the third channel, and the second channel and the fourth channel are lo...

Embodiment 2

[0026] The flow resistance matching area of ​​the Tesla valve is formed by connecting three T45C Tesla valves in sequence; the length of the flow resistance matching area of ​​the Tesla valve is 18700 μm, which is the distance from the bifurcation of the connection structure to the front end of the first-stage waste liquid outlet. distance, or the distance between the first and last ends of the three Tesla valves, such as figure 2 shown. The T45C valve consists of a main flow channel, the first and second flow channels bifurcated from the main flow channel, and an arc-shaped flow channel connected between the other ends of the first and second flow channels; the first flow channel is along the The direction of the main flow channel extends; the angle formed between the first and second flow channels is 45 degrees; the length of the first flow channel is 2500 μm; the width of each flow channel is 500 μm. With the Tesla valves of the above models and sizes and the above combin...

Embodiment 3

[0028]Both the first-level inertial sorting unit and the second-level inertial sorting unit adopt passive inertial microfluidic cell sorters, which have a double-helical channel structure, and the channel width of the first-level inertial sorting unit is 900 μm. The channel width of the secondary inertial sorting unit is 600 μm. The passive inertial microfluidic cell sorter is the prior art, but the present invention chooses the passive inertial microfluidic cell sorter with the above size, which can exert its maximum inertial focusing effect.

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Abstract

The invention discloses a multi-stage inertial micro-fluidic blood sample processing chip integrating a micro-mixer and a Tesla valve, relates to an integrated implementation method of rapid volume expansion and target cell screening of a micro-collected blood sample, and particularly relates to integrated design and preparation of the micro-mixer, a two-stage inertial cell sorting unit and a Tesla valve flow resistance matching unit. According to the invention, series connection of two stages of inertial micro-fluidic units is realized by utilizing a flow resistance matching method, and high-precision enrichment and screening of rare cancer cells in a trace blood sample are realized by combining export of blood cells through a flow resistance matching flow channel with two-stage inertial focusing, and compared with the existing one-stage passive micro-fluidic cancer cell screening technology, the two-stage series scheme provided by the invention is higher in screening purity. According to an existing micro-fluidic cell manipulation technology, a blood sample needs to be diluted firstly and then injected into a micro-fluidic chip, and according to the micro-fluidic cell manipulation technology, the micro-mixer and inertial cancer cell screeners (a first-stage inertial sorting unit and a second-stage inertial sorting unit) are integrated on an integrated sheet, so that dilution of the micro blood sample and high-precision screening of cancer cells are achieved.

Description

technical field [0001] The invention relates to an integrated realization method of rapid volume expansion and target cell screening of micro-collected blood samples, specifically the integrated design and preparation of a micro-mixer, a two-stage inertial cell sorting unit, and a Tesla valve flow resistance matching unit. Background technique [0002] Currently, the methods that can be effectively used in the detection of clinical cancer status are expensive and complicated to operate, and patients often suffer a lot of pain during the detection process (such as liver puncture, etc.). In recent years, the development of microfluidic technology has provided a path for the low-cost popularization of cancer detection. The specific solution is as follows: firstly, microfluidic technology is used to enrich the circulating tumor cells in the peripheral blood of cancer patients collected in a small amount, and Remove other cell-scale impurities; secondly, use the optical detection...

Claims

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Application Information

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IPC IPC(8): B01L3/00B01F33/30B01F101/23
CPCB01L3/5027
Inventor 段俊萍臧文轩张斌珍冀苗苗屈增
Owner ZHONGBEI UNIV
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