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Tissue culture and rapid propagation method of sweet potatoes

A technology for tissue culture and rapid propagation of sweet potatoes, applied in horticultural methods, botanical equipment and methods, cultivation, etc., can solve the problems of cumbersome and complicated methods, affecting the yield and quality of sweet potatoes, and unfavorable large-scale promotion of sweet potato tissue culture technology. The effect of promoting growth and development

Pending Publication Date: 2022-01-28
GUANGDONG OCEAN UNIVERSITY +1
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Sweet potato virus disease will seriously affect the yield and quality of sweet potato. At present, shoot tip tissue culture detoxification technology is often used to deal with it. For example, there is a chemical disinfection tissue culture method for sweet potato, but the induction medium components used in this method are phytohormones. Also need to add sodium hypochlorite, penicillin, calcium propionate and many other components, this method is loaded down with trivial details and complicated, is unfavorable for the large-scale popularization of sweet potato tissue culture technology

Method used

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  • Tissue culture and rapid propagation method of sweet potatoes
  • Tissue culture and rapid propagation method of sweet potatoes
  • Tissue culture and rapid propagation method of sweet potatoes

Examples

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Effect test

Embodiment 1

[0044] Embodiment 1 A kind of tissue culture rapid propagation method of sweet potato

[0045] S1. Pre-treatment: Wash 20 300g Marsali sweet potatoes without mold and diseases and pests on the surface, respectively bathe in water at 57°C for 3 minutes, and then place them in 50% carbendazim 800 times solution after bathing in water at 51°C for 30 minutes Tumble and disinfect for 15 minutes, wash to complete the virus passivation operation (see figure 1 ); then place the sweet potatoes flat on a plastic plate, put them into an artificial climate incubator, and add water to submerge to 1 / 3 of the size of the sweet potatoes, and put them into the water at a temperature of 32°C, a light duration of 12h / d, a light intensity of 3000lx, and a humidity of 95%. Cultivate until it grows seedlings greater than 10cm, cut off the seedlings, cut off the leaves visible to the naked eye with a sterilized blade, and cut off the larger young leaves of the stem tip under a 10-fold dissecting mic...

Embodiment 2

[0048] Embodiment 2 A kind of tissue culture rapid propagation method of sweet potato

[0049] The same as the method in Example 1, the difference is that the Marsali sweet potato described in step S1 is replaced by watermelon red sweet potato.

Embodiment 3

[0050] Embodiment 3 A kind of tissue culture rapid propagation method of sweet potato

[0051] The same as the method in Example 1, the difference is that the indolebutyric acid in step S3 is replaced by 3-naphthylacetic acid.

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Abstract

The invention provides a tissue culture and rapid propagation method of sweet potatoes. The method specifically comprises the following steps: firstly, carrying out virus passivation and water culture on sweet potatoes to obtain stem tips, sequentially carrying out alcohol disinfection and 2.5-3.5 wt% calcium hypochlorite solution disinfection, putting the stem tips into an MS culture medium, and carrying out sealed culture to obtain seedlings, wherein the MS culture medium contains 6-benayl aminopurine with the concentration of 0.45 mg / L to 0.55 mg / L, and further contains indolebutyric acid or 3-naphthylacetic acid with the concentration of 0.08 mg / L to 0.12 mg / L. According to the method, the survival rate, the seedling rate and the callus formation rate of the stem tips of the sweet potatoes can be remarkably increased, growth and development of the stem tips are promoted, and industrial production of the sweet potatoes is facilitated.

Description

technical field [0001] The invention belongs to the technical field of tissue culture. More specifically, it relates to a method for tissue culture and rapid propagation of sweet potatoes. Background technique [0002] Sweet potato (Ipomoea batatas (L.) Lam), also known as sweet potato, sweet potato, sweet potato, sweet potato, sweet potato, etc., is a herbaceous vine of the genus Ipomoea in the family Convolvulaceae. Sweet potato tastes sweet, nutritious, easy to digest, rich in starch, dietary fiber, carotene, VA, VB, VC, VE, potassium, iron, copper, selenium and calcium, etc. Swelling, anti-diabetic, anti-cancer and cholesterol-inhibiting effects. [0003] Sweet potato virus disease will seriously affect the yield and quality of sweet potato. At present, shoot tip tissue culture detoxification technology is often used to deal with it. For example, there is a chemical disinfection tissue culture method for sweet potato, but the induction medium components used in this me...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00A01G31/00
CPCA01H4/008A01H4/002A01G31/00
Inventor 陈苗林建奔曾永青江浩滨林泽彬朱松涛周川刘婷婷刘颖梁祥晶吴湛云
Owner GUANGDONG OCEAN UNIVERSITY
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