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Bacillus siamensis and application thereof

A technology of bacillus and strains, which is applied in the fields of agricultural microbiology technology and environmental protection, can solve the problems of difficult denitrification of non-point water bodies, difficulty in reducing nitrous oxide emissions, etc., and achieve the effects of reducing N2O emissions, promoting crop growth, and reducing content

Active Publication Date: 2022-01-28
NANJING UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Purpose of the invention: To provide a strain of Bacillus siamese and its application in view of the difficulties in reducing the emission of greenhouse gas nitrous oxide in farmland soil, and the difficulty in denitrification of nitrate-type secondary salinized soil and non-point source water in the prior art

Method used

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  • Bacillus siamensis and application thereof
  • Bacillus siamensis and application thereof
  • Bacillus siamensis and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0029] Isolation and screening of embodiment 1 Bacillus siamese NRCB026

[0030] The test soil was collected from the rice rhizosphere during the harvest period in Huizhou, Guangdong on November 25, 2018. Weigh 1g of soil, put it into a sterilized Erlenmeyer flask in an ultra-clean bench, and add 100ml of 0.86% physiological saline, mix well, and place it in a biochemical incubator at 28°C for static cultivation. After 7 days, transfer the Erlenmeyer flask to a shaker at 28°C at 200rpm and incubate for 20min, absorb 80ml of the supernatant, and add 40ml of 2.1μmol l -1 Sodium nitrate solution and 1.4 μmol l -1 Sodium succinate solution, 28 ℃, static culture 24h. Take 1ml of the soil suspension, put it into a conical flask filled with 9ml sterile water, mix well, and prepare a dilution of 10 -1 soil suspension; take 10 -1 Put 1ml of the soil suspension into a conical flask filled with 9ml sterile water, mix well, and make a dilution of 10 -2 soil suspension; and so on. Di...

Embodiment 2

[0031] The identification of embodiment 2 bacillus siamese NRCB026

[0032] When cultured on NBNS agar medium, the colony of NRCB026 strain was round, milky white, opaque and raised, easy to provoke; Gram staining was positive. Through 16S rDNA, gyrA and gyrB (see Table 4 for primers) sequence analysis, after BLAST alignment in NCBI, they are homologous to Bacillus siamese, see figure 1 ; See SEQ ID No.1 for 16S rDNA, gyrA and gyrB sequences. The physiological and biochemical characteristics are shown in Table 1.

[0033] Table 1 Physiological and biochemical properties of Bacillus siamese BRCB026

[0034]

[0035]

[0036] Note: "+" is exploitable or sensitive, "-" is not exploitable or insensitive, "\" is between "+" and "-"

[0037] Bacillus siamese NRCB026 was deposited in the General Microorganism Center of China Microbiological Culture Collection Management Committee, the strain preservation number is CGMCC NO.22841, and the preservation date is July 07, 2021.

Embodiment 3

[0038] Example 3 Analysis of salt tolerance of Bacillus siamese NRCB026 under pure culture system conditions.

[0039] On the basis of LB solid plate medium, different salinity levels were added, and the salt tolerance of the strain was determined by observing the growth of the strain. Exogenously added salinity levels of 0.2, 0.4, 0.6, 0.8 and 1.0 mol l -1 NaCl. The strains were respectively streaked into the culture medium with different salinity, and cultured in a constant temperature incubator at 30°C for 48h. The ability of the strain to grow is considered to be the basis for the tolerance of the corresponding salinity level. Three independent parallel experiments were performed for each treatment. See Table 2 for the growth of Bacillus siamese NRCB026 in media with different salt concentrations. When the NaCl concentration is 1mol l -1 , the strain NRCB026 can grow normally, indicating that Bacillus siamese NRCB026 can tolerate 1mol l -1 above the salt concentratio...

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Abstract

The invention discloses a strain of bacillus siamensis, wherein the bacillus siamensis is classified and named as bacillus siamensis, the strain name is NRCB026, the bacillus siamensis is preserved in the China General Microbiological Culture Collection Center, the strain preservation number is CGMCC NO.22841, and the preservation date is July 7, 2021. The bacillus siamensis disclosed by the invention can be applied to agricultural planting, and nitrate nitrogen and nitrite nitrogen are reduced into gaseous nitrogen, so that the soil quality is improved; and meanwhile, the strain effectively promotes the growth of crops and reduces the emission of nitrous oxide in soil.

Description

technical field [0001] The invention belongs to the field of agricultural microorganism technology and environmental protection, and relates to a strain of bacillus siamese and its application. Background technique [0002] With the continuous development of my country's national economy, climate and water pollution issues have increasingly become the focus of attention. The greenhouse gas nitrous oxide (N 2 O) the greenhouse effect is CO 2 150-200 times of that, and it has a long retention time and damages the ozone layer and has attracted much attention. N released during agricultural production 2 O is the main anthropogenic source of its production. Inoculation of microorganisms with nitrous oxide emission reduction effect is the 2 Effective means of O emissions. At the same time, 30-50% of the applied soil fertilizer enters the groundwater through soil leaching, and the content of nitrate and nitrite in the groundwater increases accordingly. Excessive nitrogen-con...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C02F3/34C09K17/14A01N63/22A01P21/00C12R1/07C09K101/00C09K109/00C02F101/16C02F103/20
CPCC12N1/20C02F3/34C09K17/14A01N63/22C02F2101/163C02F2101/166C02F2103/20C09K2101/00C09K2109/00Y02W10/10
Inventor 高南张欢欢徐虹牛欢青李珊珊马长义牟新飞陈洁雷鹏王瑞谷益安
Owner NANJING UNIV OF TECH
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