Walnut peptide as well as preparation method and application thereof

A technology of walnut peptide and walnut protein, which is applied in the field of walnut peptide and its preparation, can solve the problems of ignoring the difference of protease, unsatisfactory enzymatic hydrolysis effect, and low quality of walnut peptide, achieve high antioxidant activity, and improve learning and memory ability , low cost effect

Active Publication Date: 2022-02-15
中食都庆(山东)生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The existing walnut peptide extraction process has the problems of high cost, large waste, and unfavorable industrialization, and the enzymatic hydrolysis process often uses various proteases to participate in the entire enzymatic hydrolysis process, ignoring the differences between proteases, so there is an enzymatic hydrolysis effect Not ideal, the quality of the prepared walnut peptide is not high

Method used

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  • Walnut peptide as well as preparation method and application thereof
  • Walnut peptide as well as preparation method and application thereof
  • Walnut peptide as well as preparation method and application thereof

Examples

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preparation example Construction

[0052] The present invention also provides the preparation method of above-mentioned walnut peptide, comprises the following steps:

[0053] (1) Enzymolyze walnut pulp with cellulase and pectinase, inactivate the enzyme, filter and dry to obtain walnut protein;

[0054] (2) Prepare walnut protein slurry, heat the walnut protein slurry at 50-60°C, adjust the pH, and then use alkaline protease and papain to enzymolyze it to obtain a walnut protein enzymatic hydrolysis solution;

[0055] (3) adding activated carbon to the walnut protein enzymatic hydrolyzate for heat preservation, filtering, and drying to obtain a walnut peptide mixture;

[0056] (4) Prepare the walnut peptide mixture solution, and use high performance liquid phase method to separate and purify the walnut peptide.

[0057] In the present invention, the walnut meal is a defatted walnut meal obtained after shelling walnuts and removing oil by cold pressing. The protein content is 45-56%, preferably 50-55%. The speci...

Embodiment 1

[0082] (1) Double-enzyme compound extraction of walnut protein: take 1200kg defatted walnut meal, mix it with deionized water at a ratio of 1:10g / mL, adjust the pH to 6.5, raise the temperature to 54°C, add cellulase (1500U / mL) and pectin Enzyme (500U / mL), hydrolyze for 4h, inactivate enzyme at 100°C for 10min, use a plate and frame filter press to collect the filter cake, and use a tube bundle dryer to dry the filter cake to obtain walnut protein.

[0083] (2) Production process of walnut peptide:

[0084] a. Take 500kg of walnut protein, add 10000L of deionized water to make slurry.

[0085] b. Stir the walnut protein solution in step a evenly, use NaOH with a concentration of 10mol / L to adjust the pH to 9.0, then use a colloid mill (6000 rpm, filter screen 60 mesh) to process for 10 minutes, and then use a high-pressure homogenizer (7Kpa) for 6min.

[0086] c. Feed the homogenate after high-pressure homogenization, carry out stirring treatment, and raise the temperature t...

Embodiment 2

[0103] The difference between this example and Example 1 is only that: in step (1), the defatted walnut meal and deionized water are mixed at 1:11 g / mL.

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Abstract

The invention discloses a walnut peptide as well as a preparation method and application thereof, belonging to the technical field of bioactive peptides. The walnut peptide disclosed by the invention is a single-chain polypeptide with a molecular weight of 1189.186 Da, and the structural sequence of the walnut peptide is GEEREERGLD. The preparation method comprises the following steps: carrying out composite enzymolysis on walnut meal slurry to obtain walnut protein, sequentially adding alkaline protease and papain into a walnut protein solution for enzymolysis to obtain a walnut protein slurry enzymolysis product, and carrying out separating and purifying by adopting high-performance liquid chromatography to obtain the walnut peptide. The walnut peptide provided by the invention has relatively high antioxidant activity, has an obvious protection effect on PC12 cells induced by H2O2, and also has a protection effect on the central nervous system; and the walnut peptide provided by the invention can effectively improve the space memory and learning and exploration capabilities of experimental animals.

Description

technical field [0001] The invention relates to the technical field of bioactive peptides, in particular to a walnut peptide and its preparation method and application. Background technique [0002] Walnut, also known as walnut, Qiang peach, is a Juglandaceae plant. Together with almonds, cashew nuts and hazelnuts, it is also known as the world-renowned "four major dried fruits". Walnuts are rich in nutrients, with more fat per hectogram, 15-20 grams of protein, and 10 grams of carbohydrates; Flavin and other multivitamins are very popular among people. [0003] Walnut meal is a by-product of walnut oil extraction, which is only used as feed or fertilizer at present, which makes the nutrients in walnut meal not fully utilized, especially the biologically active substances in it are ignored. The utilization rate of walnut meal with high nutritional value is not high, resulting in low value-added products. In recent decades, the development of bioactive peptides has brough...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K7/06C07K1/16C07K1/14C12P21/06
CPCC07K7/06C12P21/06
Inventor 张钊张九勋李庚张学军张西平魏玮赵冉周喜高左喜梅
Owner 中食都庆(山东)生物技术有限公司
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