Single cell automatic transfer method without visual feedback
A visual feedback and single-cell technology, which is applied in the field of cell manipulation, can solve the problems that cannot be applied and cannot provide microscopic visual feedback
Pending Publication Date: 2022-03-04
NANKAI UNIV
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Problems solved by technology
However, this approach cannot be applied to work environments where microscopic visual feedback cannot be provided
Method used
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Embodiment 1
[0029] Embodiment 1: as figure 1 As shown, a single cell automatic transport method without visual feedback, including the following steps:
[0030] S1, by performing a microtube blockage experiment on a valve-type or piston-type air pump system equipped with an air pressure sensor, measure the pressure change curves in the microtubes before and after the microtubes with different inner diameters are blocked, and determine the detectable pressure inside the tube when the cells are stuck The required microtube inner diameter range under the change;
[0031] S2, according to the microtubule inner diameter range determined in step S1, screen the cell type whose diameter is larger than the smallest microtubule inner diameter within the range as the applicable object of the single-cell automatic transport method without visual feedback;
[0032] S3, making a double-layer microtube suitable for the cell type determined in step S2: the double-layer microtube is composed of an outer ...
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Abstract
The invention relates to a single cell automatic transfer method without visual feedback, which comprises the following steps: S1, through a microtube blockage experiment, determining the required microtube inner diameter range under detectable in-tube pressure change when cells are stuck; s2, screening a cell type of which the diameter is greater than the inner diameter of the minimum microtube in the range as an applicable object of the visual-feedback-free single cell automatic transfer method; s3, manufacturing a double-layer microtube for transferring suitable for the cell type determined in the step S2; s4, carrying out cell suction and outpouring experiments, and determining a pressure intensity change curve in the double-layer microtube when the cells are stuck and are outpoured; and S5, establishing a robotized non-visual feedback cell transfer process to realize automatic cell transfer. The inner diameters of the inner-layer microtube and the outer-layer microtube of the double-layer microtube are determined through a microtube blocking experiment and the size of a cell to be operated, and robotized single cell transfer without visual feedback is realized for the first time according to a pressure change curve in the tube in the process that the cell is clamped and injected out by the double-layer microtube.
Description
technical field [0001] The invention relates to the field of cell manipulation, in particular to a single cell automatic transport method which is especially suitable for large cells and does not require microscopic visual feedback. Background technique [0002] In embryo freezing and cell washing, operators need to use glass microtubes to aspirate cells and move them into new droplets, and then inject cells. This cell transfer technique is one of the most common steps in cell processing. At present, in both manual and automated cell transfer operations, it is necessary to use microscopic visual feedback to assist in judging whether cells are sucked into the microtube, and then control the air pressure of the syringe to complete the suction and injection of cells. However, this approach cannot be applied to working environments where microscopic visual feedback cannot be provided. Therefore, it is necessary to develop a new automated cell transport system that does not rely...
Claims
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IPC IPC(8): C12M1/26C12M1/34C12M1/36G06T11/20
CPCC12M33/04C12M41/40C12M41/48G06T11/203
Inventor 赵启立赵新张艺荻邱金禹李明慧孙明竹
Owner NANKAI UNIV