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NMDA receptor constructs for detection and isolation of nmdar antibodies

A technology of autoantibodies and constructs, applied in the direction of receptors of nerve media, antibody mimics/scaffolds, receptors/cell surface antigens/cell surface determinants, etc., can solve the problem of limited sensitivity of antigen detection

Pending Publication Date: 2022-03-04
德国神经退行性疾病中心
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  • Abstract
  • Description
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  • Application Information

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Problems solved by technology

However, the detection sensitivity of this antigen may be limited

Method used

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  • NMDA receptor constructs for detection and isolation of nmdar antibodies
  • NMDA receptor constructs for detection and isolation of nmdar antibodies
  • NMDA receptor constructs for detection and isolation of nmdar antibodies

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Embodiment Construction

[0102] All cited documents of patent and non-patent literature are hereby incorporated by reference in their entirety.

[0103] The present invention relates to a soluble NMDAR protein construct comprising one or more NMDAR autoantibody epitopes, wherein the construct comprises the extracellular domain (ECD) of NMDAR subunit GluN1 or a fragment thereof and NMDAR subunit GluN2A, The ECD of at least one of GluN2B, GluN2C or GluN2D or a fragment thereof.

[0104] In the context of the present invention, the term "protein construct" may refer to a single protein or peptide formed from a single chain of amino acids. Furthermore, the term "protein construct" as used herein also includes constructs or complexes of two or more proteins or peptides or amino acid chains covalently linked, eg, by disulfide bonds or other linkers between the individual amino acid chains. Furthermore, the term "protein construct" includes protein complexes formed by more than one protein or peptide or ami...

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Abstract

The present invention relates to a soluble N-methyl-D-aspartate receptor (NMDAR) protein construct comprising one or more NMDAR autoantibody epitopes wherein the construct comprises an extracellular domain (ECD) of the NMDAR subunit GluN1 or a fragment thereof and an ECD of at least one of the NMDAR subunits GluN2A, GluN2B, GluN2C or GluN2D or a fragment thereof. Furthermore, the invention relates to an in vitro method for detecting an NMDAR autoantibody in a sample, said method comprising: a.) providing a sample suspected of comprising an NMDAR autoantibody, b.) providing an NMDAR protein construct of the invention as a capture molecule, c.) contacting said sample with said NMDAR protein construct, and d.) determining the presence and optional amount of the NMDAR autoantibody bound to the NMDAR protein construct, thereby binding the NMDAR autoantibody from the sample to the NMDAR protein construct, and d.) determining the presence and optional amount of the bound NMDAR autoantibody. In embodiments, the methods of the invention are useful in the diagnosis, prognosis, disease monitoring, patient stratification and / or treatment monitoring of medical conditions associated with autoantibodies against NMDAR, preferably anti-NMDAR encephalitis.

Description

[0001] describe [0002] The present invention relates to a soluble N-methyl-D-aspartate receptor (NMDAR) protein construct comprising one or more NMDAR autoantibody epitopes, wherein the construct comprises the NMDAR subunit GluN1 The extracellular domain (ECD) or fragment thereof and the ECD or fragment thereof of at least one of the NMDAR subunits GluN2A, GluN2B, GluN2C or GluN2D. Furthermore, the present invention relates to an in vitro method for detecting NMDAR autoantibodies in a sample, said method comprising: a.) providing a sample suspected of comprising NMDAR autoantibodies, b.) providing an NMDAR protein construct of the present invention, as a capture molecule, c.) contacting said sample with said NMDAR protein construct, whereby NMDAR autoantibodies from said sample bind to said NMDAR protein construct, and d.) determining the presence of bound NMDAR autoantibodies and an optional amount. In an embodiment, the method of the invention is used for the diagnosis, pr...

Claims

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Application Information

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IPC IPC(8): C07K14/705G01N33/68
CPCC07K14/705G01N33/6893G01N2800/24C07K2319/00G01N33/564G01N2800/52G01N2800/7095G01N2800/28C07K14/70571C07K2319/40C07K2319/30G01N2333/70571
Inventor 汉斯-克里斯蒂安·科尔瑙哈拉尔德·普吕斯克雷格·柯蒂斯·加尔内塔尼塔·弗雷
Owner 德国神经退行性疾病中心
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