PIK3CA gene and BRAF gene mutation multiple detection primer probe and kit thereof

Abstract

The invention relates to a PIK3CA gene and BRAF gene mutation multiple detection primer probe and a kit thereof. The PIK3CA gene and BRAF gene mutation multiple detection primer probe comprises an upstream primer F-T790M, a downstream primer R-T790M, a wild type probe WP-T790 and a mutant type probe MP-T790M, wherein the upstream primer F-T790M, the downstream primer R-T790M, the wild type probe WP-T790 and the mutant type probe MP-T790M are used for detecting PIK3CA gene and BRAF gene No. 20 exon T790M mutation; the kit comprises an upstream primer F-L858R, a downstream primer R-L858R, a wild type probe WP-L858 and a mutant type probe MP-L858R which are used for detecting mutation of No. 21 exons L858R of the PIK3CA gene and the BRAF gene, and an upstream primer F-E19del, a downstream primer R-E19del, a wild type probe WP-E19 and a mutant type probe MP-E19del which are used for detecting deletion mutation of No. 19 exons of the PIK3CA gene and the BRAF gene. The PIK3CA gene and BRAF gene mutation multiple detection primer probe and the kit thereof have the advantages of rapidness, high efficiency, simple preparation of a standard substance, high specificity and sensitivity of a detection system, high utilization rate of free DNA, and high accuracy.

Description

technical field [0001] The invention relates to a PIK3CA gene and BRAF gene mutation detection product, which belongs to the field of biotechnology. Background technique [0002] The EGFR gene mainly has two downstream signal transduction pathways, the first of which is the Ras / Raf / MEK / ERK-MAPK pathway. The PIK3CA gene is a lipid kinase-encoding gene, and it is a high-frequency somatic mutation gene in various tumors such as colorectal cancer, ovarian cancer, breast cancer, gastric cancer, liver cancer, and lung cancer. Genes encoding effectors in the mitogen-activated protein kinase (MAPK) signaling pathway activate somatic alterations with high frequency (70%), including point mutations in members of the phosphoinositide 3-kinase (PI3K) pathway, such as PTEN, PIK3CA and AKT1. Studies have pointed out that PIK3CA gene mutation is one of the common gene mutations in breast cancer besides HER2 gene amplification and p53 gene mutation. Nearly 80% of the mutations occur in th...

AI Technical Summary

Problems solved by technology

However, the traditional method has low sensitivity and strict requirements on sample types, and is only suitable for surgical tissue samples, which makes it impossible to continuously and real-time observe the mutation status of genes

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