Screening method and application of tussah breeding material

Abstract

The invention belongs to the technical field of silkworm breeding, and particularly relates to a method for screening antheraea pernyi breeding materials and application thereof.The method includes the steps that the antheraea pernyi breeding materials of different varieties are selected, seminal fluid generated in the mating process of male and female seed moths is obtained, acrosomal enzyme activity determination is conducted on the collected and reserved seminal fluid, and after the female moths of the breeding materials of the various varieties lay eggs, the female moths of the breeding materials of the various varieties lay eggs. The practical hatching rate of the tussah eggs of each variety is counted, a regression equation is established according to the relationship between the acrosin enzyme activity of the sperms of each variety and the practical hatching rate of the tussah eggs, and the acrosin enzyme activity of the tussah sperms of different breeding materials can be measured in the'two-generation and one-release 'production period of the tussah. The practical hatching rate of the silkworm eggs is predicted according to the established linear regression equation, so that the male breeding material with the high practical hatching rate of the silkworm eggs is screened out and used for breeding of the'two-stage and one-stage 'tussah varieties, and the male breeding material screened out in the mode can mate to obtain seed eggs with the high practical hatching rate of the tussah eggs.

Description

technical field [0001] The invention belongs to the technical field of silkworm breeding, and in particular relates to a method for screening tussah silkworm breeding materials and an application thereof. Background technique [0002] Tussah, also known as mountain silkworm, originated in the south-central region of Shandong Province in my country. As the birthplace of tussah industry, China has always maintained the status of the world's largest tussah industry. The national total output accounts for more than 90% of the world's total output, of which The contribution rate of Liaoning Province is more than 60%. In addition to Liaoning, Jilin, Heilongjiang and Inner Mongolia are also rich in tussah forest resources. The total area of ​​tussah forest suitable for stocking tussah in these four provinces is 3.463 million hm 2 , but the actual application area is only 684,000 hm 2 , the utilization rate is less than 20%. The objective reason is that these provinces are located i...

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Problems solved by technology

[0002] Tussah, also known as mountain silkworm, originated in the south-central region of Shandong Province in my country. As the birthplace of tussah industry, China has always maintained the status of the world's largest tussah industry. The national total output accounts for more than 90% of the world's total output, of which The contribution rate of Liaoning Province is more than 60%. In addition to Liaoning, Jilin, Heilongjiang and Inner Mongolia are also rich in tussah forest resources. The total area of ​​tussah forest suitable for stocking tussah in these four provinces is 3.463 million hm 2 , but the actual application area is only 684,000 hm 2 , the utilization rate is less than 20%. The objective reason is that these provinces are located in the north, and the frost-free period is short. As the two-season tussah silk production area, it is impossible to safely stock two seasons of tussah. large area idle

However, in practical application, it is found that after long-term low-temperature storage of seed cocoons, the hatching rate of silkworm eggs will be significantly reduced. The hatching rate of silkworm eggs is the main trait index of the vitality of tussah silkworm eggs, and it is also one of the main economic indicators of tussah silkworms. Therefore, , the significant reduction in the practical hatching rate of tussah eggs has also become a major problem restricting the development of the "two chemical and one release" production model, making the scale of "two chemical and one release" silkworm breeding only account for about 10% of the stocking area in suitable areas

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