Enterobacter cloacae and application thereof

A technology of Enterobacter cloacae and active bacteria is applied in the field of radioactive pollution control of uranium mines, which can solve the problems of long cycle, inability to meet the pollution control of uranium tailings, secondary disposal, etc., to reduce the risk of migration, inhibit the generation of uranium pollution, Effective repairing action

Pending Publication Date: 2022-03-29
THE FOURTH INST OF NUCLEAR ENG OF CNNC
View PDF5 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The above-mentioned studies on uranium pollution control are generally small in number, and most of them focus on the remediation of uranium-contaminated soil mainly based on plants. Since the soil is secondary pollution caused by uranium tailings and leaching water, its uranium content Relatively low, and phytoremediation has the defects of low efficiency, long cycle and the need for secondary disposal, which cannot meet the needs of uranium tailings pollution control
In addition to the lime neutralization treatment method in China, there is no effective technical method that can be used to control the source pollution of uranium tailings pollution. Therefore, it is necessary to develop a new technology that is environmentally friendly, low-cost, effective, and can be popularized. Urgent needs in the field of radioactive pollution control of uranium mines

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Enterobacter cloacae and application thereof
  • Enterobacter cloacae and application thereof
  • Enterobacter cloacae and application thereof

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0041] The embodiment of the present invention also discloses a preparation method of active bacterial liquid, comprising the following steps:

[0042] Step S1: Inoculate Enterobacter cloacae with the preservation number of CGMCC No: 20602 in the liquid medium, and culture it statically to obtain the basic active bacterial agent;

[0043] Step S2: inoculate the basic active bacterial agent in the fermentation medium according to the inoculation amount of 2-5%, and carry out shaking culture, so that the number of cells in each milliliter of fermentation medium is not less than 3ⅹ10 9 ~5ⅹ10 9, to obtain the active bacterial liquid.

[0044] The present invention obtains active bacterial liquid through strain fermentation and liquid culture. After the activity of the functional bacterial liquid is strengthened, a functional bacterial agent can be obtained, which can be used for the treatment and restoration of uranium tailings.

[0045] The following steps illustrate the prepa...

Embodiment 1

[0064] The frozen Enterobacter cloacae with preservation number 20602 was quickly thawed at 37°C, put into a test tube containing 15mL of liquid medium A according to the inoculum amount of 1%, and cultured statically at 30°C for 18 hours to obtain Basic active bacterial agent;

[0065] The liquid medium A consists of: 3-5g NaCl, 8-10g tryptone, 8-10g yeast extract, 1000mL distilled water, pH 6.8-7.0;

[0066] Insert the basic active bacterial agent into a sealed Erlenmeyer flask containing 200-250mL medium B according to the inoculum amount of 2-5%, adopt 28-32°C, and rotate at a speed of 60-100rpm, shake and cultivate for 5-7 days, so that No less than 3ⅹ10 cells per ml 9 , so as to obtain the active bacterial liquid;

[0067] The composition of the fermentation medium B is: 2-5 mL of ethanol, K 2 HPO 4 0.2~0.5g, (NH 4 ) 2 SO 4 2~2.5g,Na 2 SO 4 1~2g, CaCl 2 0.05~0.1g, MgSO 4 ·7H 2 O 0.05~0.1g, L-cysteine ​​hydrochloride 0.5~1g, water 1000mL, pH 6.8~7.0.

[0...

Embodiment 2

[0071] After the uranium tailings are air-dried, pass through a 5-mesh sieve, mix well and put them into a plastic barrel with a diameter of 16 cm and a height of 30 cm. Divide into two barrels for sub-package, and each barrel is filled with 1.5kg of uranium tailings. The uranium content in the tested uranium tailings was 0.58mg / kg, and the pH was 6.93.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention relates to the field of uranium mine radioactive pollution treatment, in particular to enterobacter cloacae and application thereof. The collection number of the enterobacter cloacae is CGMCC (China General Microbiological Culture Collection Center) NO: The enterobacter cloacae is applied to uranium element reduction and uranium ore slag pollution treatment. The invention provides a preparation method of active bacterial liquid, which comprises the following steps: inoculating enterobacter cloacae into a liquid culture medium, standing and culturing to obtain a basic active bacterial agent; and inoculating the basic active bacterial agent into a fermentation medium according to an inoculation amount of 2-5%, and carrying out shake culture, so that the number of cells in each milliliter of the fermentation medium is not less than 3 * 10 < 9 >-5 * 10 < 9 >, thereby obtaining the active bacterial liquid. The strain has a reduction effect on uranium in tailings, uranium is converted into a reduction state which is not easy to dissolve from an oxidation state which is easy to dissolve, and the uranium is fixed in situ, so that the risk of migration to the external environment is reduced, and uranium pollution is inhibited from the source.

Description

technical field [0001] The invention relates to the field of radioactive pollution control of uranium mines, in particular to enterobacter cloacae and its application. Background technique [0002] Uranium tailings are the largest amount of solid radioactive pollutants produced during uranium mining and smelting in my country. Uranium tailings are the residues of the acid hydrometallurgy process, most of which are obviously acidic; the residual uranium in the tailings is in an unstable state after being washed and leached by water for a long time, and is easy to dissolve. At present, the uranium tailings are mainly treated by the method of lime neutralization and storage after disposal. However, in long-term engineering practice, it has been found that lime neutralization cannot effectively maintain stability for a long time, and acid reflux will occur, which will further reduce the pH value of the seepage water, continue to dissolve uranium, and increase the concentration....

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20B09C1/10C09K17/14C12R1/01C09K109/00
CPCC12N1/20B09C1/10C09K17/14C09K2109/00
Inventor 安毅夫孙娟高扬连国玺
Owner THE FOURTH INST OF NUCLEAR ENG OF CNNC
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap