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Preparation and synchronous modification method of novel multifunctional embolism

A modification method and multi-functional technology, which can be used in medical science, surgery, surgical adhesives, etc., can solve the problems of inability to be visualized under X-ray, lack of adhesion, etc., and achieve good dispersion, permanent unity, and shape. Complete smooth effect

Active Publication Date: 2022-04-08
SHANGHAI FIRST PEOPLES HOSPITAL +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] Interventional surgery has played a pivotal role in clinical practice. Polyvinyl alcohol (PVA) particles are commonly used solid embolisms in interventional procedures. Adhesion and inability to be visualized under X-ray

Method used

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  • Preparation and synchronous modification method of novel multifunctional embolism
  • Preparation and synchronous modification method of novel multifunctional embolism
  • Preparation and synchronous modification method of novel multifunctional embolism

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Liquid paraffin (50 mL) containing sorbitan monooleate (0.9 g) was used as the continuous phase, and 10% PVA (type 1788) aqueous solution was used as the dispersed phase. The dispersed phase was added dropwise to the continuous phase at 60°C, and stirred magnetically for 30 min to obtain an emulsion.

[0029] Then, glutaraldehyde (1.8 mL, 25%) was added and stirred for 10 min, hydrochloric acid solution (1M, 1.5 mL) was used as a catalyst for crosslinking for 5 h, the PVA microspheres were recovered by centrifugation, and washed three times with ethanol and distilled water respectively.

[0030] Immediately, the lyophilized microspheres were filtered through a standard sieve (150 mesh) at 0.8mol / L I 2 / KI (1:2) aqueous solution soaked at room temperature for 24 hours, rinsed with water 3 times after removing the solution.

[0031] Next, 0.2 g of dopamine hydrochloride (DA·HCl) was dissolved in Tris buffer (40 mL, 0.01 mol / L, pH=8.5), and 100 mg of the above-mentioned p...

Embodiment 2

[0034] The morphology of freeze-dried PVA and I-PVA@PDA microspheres was observed by SEM. 100 microspheres were randomly selected from the SEM images, and the average particle size and particle size distribution of the microspheres were analyzed using Image J software. like figure 1 It can be seen that the PVA and I-PVA@PDA microspheres are spherical and complete, the surface is smooth, and there is no agglomeration, and the morphology of the two microspheres is very similar. The average diameters of PVA microspheres and I-PVA@PDA microspheres were 133.7±41.9 μm and 147.9±42.0 μm, respectively.

[0035] The chemical bond composition of PVA and I-PVA@PDA microspheres was determined by FTIR. Specifically, the sample is fully mixed with potassium bromide, pressed by a tablet machine, and tested by an infrared spectrometer at 4000-500cm -1 range of FTIR spectra. analyze figure 2 , at 3550-3200cm -1 The large bands observed in between are associated with O-H stretching vibra...

Embodiment 3

[0039] Cytocompatibility and hemocompatibility tests of I-PVA@PDA microspheres. The cytotoxicity of I-PVA@PDA microspheres was determined by the L929 indirect contact method. Different concentrations (10 mg / mL, 20 mg / mL and 40 mg / mL) of I-PVA@PDA microspheres were dispersed in DMEM and incubated at 37 °C, 5%CO 2 Microsphere extracts were prepared by incubating in an incubator for 24 h. L929 with 8×10 3 The density of cells / well was seeded on a 96-well culture plate, and 100 μL of DMEM microsphere extract was added to the well after 24 hours.

[0040] After 24 hours, the medium was removed, and 10 μL of CCK-8 in DMEM was added to each well, and incubated for another 2 hours. The absorbance at 450 nm of the solution was measured by a microplate reader, and the relative viability of the cells was measured by the CCK-8 method. The morphology and survival of L929 cells were observed with an inverted phase-contrast microscope. The test results showed that the relative cell viab...

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Abstract

The invention discloses a preparation and synchronous modification method of a novel multifunctional embolism, and particularly relates to research and development of embolism microspheres which are stable in structure, proper in size and capable of coating an imaging object with polydopamine, and visualization is realized on the basis of enhancing adhesion and treatment effects. In-vitro experiments prove the biocompatibility and the X-ray imaging capability of the material. The microspheres can also load chemotherapeutic drugs, and animal experiments prove that the microspheres have good embolism effects. The design embodies an excellent novel method for synthesizing the multifunctional embolism.

Description

technical field [0001] The invention belongs to the field of biological material preparation, and in particular relates to a preparation and synchronous modification method of a novel multifunctional embolism. Background technique [0002] Interventional surgery has played a pivotal role in clinical practice. Polyvinyl alcohol (PVA) particles are commonly used solid embolisms in interventional procedures. Attachment and lack of imaging under X-ray. [0003] In view of the lack of image-visualized adhesive solid embolic agents in current medical practice, the present invention uses PVA with good biocompatibility as a raw material, combines highly adhesive polydopamine (PDA), and simultaneously adds iodine (I) and other high-electron Density material, so as to realize the multifunctional unity of visibility, adhesion, loading and permanence of the new embolic material, and it has been verified experimentally. Contents of the invention [0004] In order to solve the above-m...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L24/06A61L24/02A61L24/00
Inventor 安潇杨雪晴张翔王世革
Owner SHANGHAI FIRST PEOPLES HOSPITAL
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