Molecular marker related to long and short pigeon fluff character and application of molecular marker
A technology of molecular markers and lengths, applied in the determination/testing of microorganisms, DNA/RNA fragments, recombinant DNA technology, etc. detection methods etc.
Active Publication Date: 2022-04-12
NANJING AGRICULTURAL UNIVERSITY
View PDF0 Cites 0 Cited by
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Most of the body, especially the head area, has short and sparse fluff, which can be directly distinguished by the naked eye, but this phenotype can only be accurately judged within seven days after hatching. indistinguishable
In the 1940s, it was found through crossbreeding experiments that the phenotype of pigeon short-hair conforms to the law of sex-linked recessive inheritance, but the research on the natal-down gene and molecular markers of this trait has not been reported yet.
If this trait is to be used in breeding work, it is necessary to use molecular markers to accurately identify the genotype of the long and short hair locus of pigeons, but there is still a lack of molecular markers and detection methods for the long and short hair traits of pigeons
Method used
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View moreImage
Smart Image Click on the blue labels to locate them in the text.
Smart ImageViewing Examples
Examples
Experimental program
Comparison scheme
Effect test
Embodiment 1
[0034] The above specific implementation manners are further described below through the examples and accompanying drawings, but the present invention is not limited to the scope of the examples.
[0035] The length and shortness of the pigeon refers to the length and density of the natal down of the pigeon about 1-7 days after hatching, such as figure 1 As shown, the left is a long-haired young pigeon, and the right is a short-haired young pigeon. In this example, a nucleotide at the 769369bp site of the pigeon Z chromosome (reference genome version Cliv_1.0) is used for single nucleotide polymorphism (SNP) detection, and the natal-down gene (natal-down) of the pigeon is determined according to the test results. genotype) and whether the gene is homozygous.
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More PUM
Login to View More Abstract
The invention relates to a molecular marker and application thereof, in particular to a molecular marker related to pigeon long and short velvet characters and application thereof, and belongs to the technical field of biology. The invention provides an SNP (Single Nucleotide Polymorphism) marker primer pair related to identification of pigeon long and short fluff characters, and provides a molecular method for detecting the pigeon long and short fluff characters by using the SNP marker primer pair. According to the present invention, the site of the SNP marker is the nucleotide of the Z chromosome 769369bp site of the pigeon reference genome version Cliv1.0, and has A / G polymorphism; the SNP marker is highly associated with long and short hair characters, the single nucleotide polymorphism can be used as a genetic marker for pigeon breeding, marker-assisted selection is carried out by utilizing a molecular biology means, and the SNP marker has important breeding value and economic value.
Description
technical field [0001] The invention relates to a molecular marker and its application, in particular to a molecular marker related to pigeon long-lint properties and its application, and belongs to the field of biotechnology. Background technique [0002] Single nucleotide polymorphism (SNP) refers to the variation of a single nucleotide in the genome, which can be stably inherited to offspring during the transmission of genetic material, and can be used as a molecular marker for breeding and conservation. [0003] Pigeons are the fourth largest poultry in my country after chickens, ducks and geese. However, compared with chickens, the progress of pigeon genetics and genomics research is relatively slow. Feathers are unique skin derivatives of poultry, which have functions such as heat preservation and flight. Feathers have different variations in color, traits, etc., and are important models for genetics and developmental studies. There are many types of feathers. Down f...
Claims
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More Application Information
Patent Timeline
Login to View More Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6888C12N15/11
Inventor 冯春刚段颖史凯鹿西学刘相平李东锋赵茜宋迟徐善金
Owner NANJING AGRICULTURAL UNIVERSITY