Application of pregabalin in preparation of medicine for preventing or treating radiation-induced brain injury

A radiation-induced brain injury, pregabalin technology, applied in the prevention or treatment of radiation-induced brain injury drugs, in the field of pregabalin, can solve the problems of unclear pharmacological effects of pregabalin, and achieve the effect of inhibiting release

Pending Publication Date: 2022-04-22
SUN YAT SEN MEMORIAL HOSPITAL SUN YAT SEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, it is unclear whether pregabalin has other potential pharmacological effects

Method used

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  • Application of pregabalin in preparation of medicine for preventing or treating radiation-induced brain injury
  • Application of pregabalin in preparation of medicine for preventing or treating radiation-induced brain injury
  • Application of pregabalin in preparation of medicine for preventing or treating radiation-induced brain injury

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Example 1 Effect of Pregabalin on Inflammatory Response in Radiation Brain Injured Mice

[0028](1) Experimental method: 3-month-old male Balb / c mice were selected, and the whole brain was irradiated with a dose of 30Gy to establish a mouse model of radiation brain injury. Pregabalin was administered intravenously to mice at a dose of 30 mg / kg body weight per day. On the 3rd and 7th days, the mice were collected, the cerebral cortex was separated, and the tissue RNA was extracted, and the mRNA levels of inflammatory factors IL-1β, IL-6, TNF-α and COX-2 were detected by Q-PCR, as shown in figure 1 As shown in A.

[0029] (2) The experimental results are as follows figure 1 Shown in B-E. Depend on figure 1 B-E shows that the mRNA levels of inflammatory factors IL-1β, IL-6, TNF-α and COX-2 in the cerebral cortex were significantly increased after radiation, and the mRNA levels of related inflammatory factors were significantly inhibited after pregabalin treatment ( fi...

Embodiment 2

[0030] Example 2 The effect of pregabalin on the activation of microglial cells in mice with radiation-induced brain injury.

[0031] (1) Experimental method: Single-cell sequencing was used to verify whether the mRNAs of inflammatory factors in the cerebral cortex after radiation were mainly expressed on microglial cells. A 3-month-old male Balb / c mouse was selected and subjected to whole brain irradiation at a dose of 30Gy to establish a mouse model of radiation brain injury. Pregabalin was administered intravenously to mice at a dose of 30 mg / kg body weight per day. On the 3rd day, 7th day and 14th day, the mice were harvested, the brain tissue was separated, fixed and dehydrated, embedded and sliced, and the size and activation of microglial cell bodies were detected by immunofluorescence experiments.

[0032] (2) The experimental results are as follows figure 2 A-I are shown. Depend on figure 2 A shows that the mRNAs of related inflammatory factors are mainly expres...

Embodiment 3

[0033] Example 3 Pregabalin inhibits the activation of microglial cells by acting on neurons.

[0034] (1) Experimental method: primary microglia and BV2 microglia were cultured and irradiated with a dose of 10Gy, and the mRNA levels of related inflammatory factors Il-1β, Il-6, TNF-α and ICAM-1 were detected And the protein expression of IL-1β and TNF-α was detected by immunofluorescence staining; the irradiated cells were treated with pregabalin, and the expressions of related inflammatory factors Il-1β, Il-6, TNF-α and ICAM-1 were detected. mRNA level, the protein expression of IL-1β and TNF-α was detected by immunofluorescence staining; the supernatant of neurons irradiated with 10Gy irradiation dose and the supernatant of neurons without irradiation were used to incubate small For glial cells, the mRNA levels of related inflammatory factors Il-1β, Il-6, TNF-α and ICAM-1 were detected, and the protein expressions of IL-1β and TNF-α were detected by immunofluorescence staini...

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Abstract

The invention discloses application of pregabalin in preparation of a medicine for preventing or treating radiation brain injury, and relates to the technical field of new application of medicines. It is found that pregabalin can inhibit increase of inflammatory factors and microglial cell activation caused by radiation brain injury in a radiation brain injury mouse model, neuron injury and apoptosis induced by inflammation are further improved, and no obvious side effect exists. The pregabalin can be used for developing a novel medicine for preventing or treating radiation brain injury, and has wide medical prospect and economic value.

Description

technical field [0001] The invention relates to the technical field of new uses of medicines, in particular to the application of pregabalin in the preparation of medicines for preventing or treating radiation-induced brain injury. Background technique [0002] Radiation therapy is one of the most effective treatments for head and neck cancer. Nasopharyngeal carcinoma (NPC) is the most common head and neck malignant tumor in my country. It has regional epidemiological characteristics, especially in the south of my country with the highest incidence rate, with 30-50 cases per 100,000 people. Most of the pathological types of nasopharyngeal carcinoma are non-keratinizing undifferentiated carcinomas, which are closely related to Epstein-Barr virus infection. Due to its anatomical structure and pathological specificity, it is highly radiosensitive, and radiotherapy has become the preferred treatment for nasopharyngeal carcinoma. Radiation therapy has been widely used clinicall...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/197A61P25/00A61P29/00
CPCA61K31/197A61K9/0019A61P25/00A61P29/00
Inventor 唐亚梅张展林伟杰邢梦丹吴敏仪石中山
Owner SUN YAT SEN MEMORIAL HOSPITAL SUN YAT SEN UNIV
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