Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Firefly luminous popular science education kit, luciferase and preparation method of firefly luminous popular science education kit

A luciferase and firefly technology, applied in the field of firefly luminescence, can solve the problems of dullness, lack of interactivity and intuition, little understanding of the principle of firefly luminescence, etc., and achieve the effect of convenient observation.

Pending Publication Date: 2022-04-29
武汉霆科生物科技有限公司
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the display form of firefly specimens adopted by the current mainstream science popularization methods is a boring form display, which lacks interactivity and intuition, and cannot better display the most important characteristics of fireflies.
In addition, what the public is most curious about about fireflies is their luminescence, but little is known about the mechanism of fireflies’ luminescence

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0034] The technical solutions in the embodiments of the present invention will be clearly and completely described below in conjunction with the embodiments of the present invention. Apparently, the described embodiments are only some of the embodiments of the present invention, not all of them. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without making creative efforts belong to the protection scope of the present invention.

[0035] The embodiment of the present invention provides a kind of luciferase, which adopts the method of recombinant prokaryotic expression to express a protein whose emission wavelength changes under different pH conditions, and the pH value is different, resulting in a slight change in the tertiary structure of the luciferase protein. As a result, the luminescent wavelength is different, orange-yellow light under acidic conditions, and bright yellow light under alkaline cond...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides luciferase, which is derived from Lucidium leucicola, has a protein characteristic of luminous wavelength change under different pH conditions, is orange-yellow light under an acidic condition and bright-yellow light under an alkaline condition, and has an amino acid sequence of SEQ ID NO.1; the luciferase is applied to the firefly luminescence science popularization education kit, so that the common public can extract a luminescence substrate (fluorescein) in a firefly body through operation by himself / herself, carry out a firefly luminescence experiment and understand the principle of firefly luminescence.

Description

technical field [0001] The invention relates to the technical field of firefly luminescence, in particular to a firefly luminescence science education kit, luciferase and a preparation method thereof. Background technique [0002] Because of their luminous properties, fireflies have become indicator organisms and star species for environmental protection, and have been included in the three protected lists. With the increasing popularity of firefly tourism and the increase in firefly protected areas, the public has gradually realized the importance of protecting fireflies. There is also an increasing demand for contact with fireflies and related science education. However, the firefly specimen display form adopted by the current mainstream science popularization methods is a boring morphological display, which lacks interactivity and intuition, and cannot better display the most important characteristics of fireflies. In addition, what the public is most curious about about...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12N9/02C12N15/70C12N15/53C12N1/21G09B23/22C12R1/19
CPCC12N9/0069C12Y113/12007C12N15/70G09B23/22
Inventor 付新华朱馨蕾刘全
Owner 武汉霆科生物科技有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products