Firefly luminous popular science education kit, luciferase and preparation method of firefly luminous popular science education kit
A luciferase and firefly technology, applied in the field of firefly luminescence, can solve the problems of dullness, lack of interactivity and intuition, little understanding of the principle of firefly luminescence, etc., and achieve the effect of convenient observation.
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[0034] The technical solutions in the embodiments of the present invention will be clearly and completely described below in conjunction with the embodiments of the present invention. Apparently, the described embodiments are only some of the embodiments of the present invention, not all of them. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without making creative efforts belong to the protection scope of the present invention.
[0035] The embodiment of the present invention provides a kind of luciferase, which adopts the method of recombinant prokaryotic expression to express a protein whose emission wavelength changes under different pH conditions, and the pH value is different, resulting in a slight change in the tertiary structure of the luciferase protein. As a result, the luminescent wavelength is different, orange-yellow light under acidic conditions, and bright yellow light under alkaline cond...
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