38 results about "Photinus pyralis" patented technology

The present invention relates to a compound having a structure analogous to firefly luciferin. In particular, the invention relates to a heterocycle compound which produces a luminescence at a light wavelength different from that of firefly luciferin in nature. The present invention provides a heterocycle compound of following general formula I. In the above general formula, R1, R2 and R3 can be each independently H or C1-4-alky. In the above general formula, X and Y can be each independently C, N, S or O. In the above general formula, the olefin chain part expressed as “n” can be changed to desired length.

The invention relates to firefly luciferase and an encoding gene and an obtaining method thereof. The defect that the original firefly luciferase in North America is poor in heat stability and cannot be used at a high temperature is overcome; and the original firefly luciferase in North America is mutated, so that mutative enzyme of which the heat stability is obviously improved is obtained; and the application value is improved. An amino acid sequence is SEQ ID NO.1; and a protein deoxyribonucleic acid (DNA) sequence in encoding is SEQ ID NO.2. An encoding gene and a preparation method of firefly luciferase mutant in North America are provided. By the method, a recombinant expression vector containing a wild firefly luciferase gene is built; and the protein is purified by using an affinity chromatography method. The method is simple and feasible, simple to operate, and low in cost.

This invention relates to: the development of a mutant firefly luciferase in order to use dATP as a DNA polymerase substrate upon pyrosequencing, such luciferase being subjected to substrate specificity modification in a manner such that the dATP-induced activity alone is decreased while the ATP-induced activity is maintained; and a mutant firefly luciferase for which the proportion of activity induced by dATP to activity induced by ATP (dATP / ATP) is lower than that for the wild-type firefly luciferase, in which an amino acid identified based on homology analysis as corresponding with the 421st amino acid (glycine) of the amino acid sequence of the wild-type North American firefly (Photinus pyralis) luciferase has been substituted with a polar amino acid.

The invention discloses a nonlinear system state estimation method, relates to the technical field of signal processing, and solves the problem of lack of particle diversity in the nonlinear system state estimation process of an existing particle filtering method. According to the method, a firefly algorithm is adopted to optimize particle distribution, and the firefly algorithm is improved in theprocess of combining the firefly algorithm and the particle filtering algorithm. According to the method, the particle diversity is effectively increased, the number of effective particles is increased, errors are reduced, the estimation precision is improved, and the method is widely applied to state estimation of a nonlinear system. According to the method, the firefly algorithm is adopted to replace a resampling process in a particle filtering method, so that the diversity of particles is kept, and the problem of lack of particle diversity is avoided. In the process of combining the firefly algorithm and the particle filtering algorithm, the standard firefly algorithm is optimized from four aspects, the quality of particles is effectively improved, the distribution of the particles iscloser to the state quantity to be estimated, and the estimation precision is improved.

The invention relates to a preparation method of radioresistant bacterium that contains the activity of luciferase. The invention takes plasmids originating from radioresistant bacterium deinococcus radiopugnans as a base to recompose with plasmids that can ensure self-duplication in escherichia coli to form a shuttle carrier. The carrier is recomposed with DNA fragments in the luciferase gene lux field that originates from into host DNA of photinus pyralis from North America into plasmids with luciferase genes. Then the plasmids are introduced into bacterial of a radioresistant bacterium family, deinococcus grandis are transformed by the constructed plasmids to get chloramphenicol resistant strains. The radioresistant bacterium prepared by the method of the invention can be used in various fields of outdoor opening and close environments. The carrier has the luciferase genes, thereby having better application value to real-time supervision and control on the propagation, distribution and influence to the environment of the recomposed transformants.

The invention relates to a path planning method, device and equipment and a storage medium, and the method comprises the steps: carrying out the gridding of a to-be-optimized region, and obtaining a grid region graph; determining the positions of a cruise starting point, a cruise passing point and a cruise ending point in the grid area map; adopting a preset firefly algorithm to carry out optimal solution solving on the positions of the cruise starting point, the cruise passing point and the cruise ending point to obtain a target cruise path; in each iteration process of the firefly algorithm, using a preset movement factor to update the position of the firefly, and the movement factor being used for expanding the search range of the firefly. According to the technical scheme provided by the embodiment of the invention, the path planning precision can be improved.

There is presented a detailed electronic method for simulation of a firefly, other insects, or organisms producing bioluminescence and with light radiation functions similar to a real firefly, other insects, or organisms producing bioluminescence. An illuminated artificial firefly, other insects, or organisms producing bioluminescence can be placed on trees and shrubs in landscape, or at home to produce radiation, at night time, and blinking a gradually decaying light. More particularly it relates to a decorative type of device in the form of a firefly, other insects, or organisms producing bioluminescence and with light radiation functions similar to a real firefly, other insects, or organisms producing bioluminescence. The light is simulated by an electronics program that is a part of compact device with the form of a real firefly, other insects, or organisms producing bioluminescence. Short radiation pulses of microsecond-millisecond duration time in violet and ultraviolet range of radiation wavelengths can be utilized for scaring birds or other animals from undesirable or dangerous places of human activity, such as airports, contaminated ponds, wind turbines, electorized fences and antennas.

A firefly larva breeding device comprises a breeding box, an adjusting mechanism, a feeding mechanism and a humidifying mechanism, the top of the breeding box is rotationally connected with a cover plate through a hinge, a storage groove and a driving groove are formed in the upper half portion and the lower half portion of the breeding box respectively, and the adaptive adjusting mechanism is arranged at the bottom of the inner circumference of the storage groove; a plurality of limiting grooves are formed in the side, away from the adjusting mechanism, of the lower half portion of the storage groove, feeding mechanisms matched with the adjusting mechanism are arranged in the limiting grooves, a turnover cover is rotationally connected to the side, close to the limiting grooves, of the breeding box, and the humidifying mechanism penetrating through the breeding box and connected with the bottom of the cover plate is arranged in the driving groove. A blow-off pipe and a water supply pipe connected with the humidifying mechanism are mounted on one side of the lower half part of each of the storage tank and the driving tank through fasteners, and a baffle is mounted on one side, close to the driving tank, of the breeding box. The firefly larva breeding device can be adjusted according to the needed breeding environment, and meanwhile feeding is convenient.

The present invention is shuttle vector between antiradiation bacterium and colibacillus and its preparation process. Antiradiation bacterium Deinococcus radiopugnans originated plasmid and the colibacillus plasmid capable of being auto replicated are recombined to constitute the shuttle vector. The shuttle vector is recombined with the DNA segment possessing luciferase gene lux originated from host DNA of Photinus pyralis to constitute plasmid with luciferase gene, the plasmid is introduced into antiradiation bacterium, and the constituted plasmid is used in converting Deinococcus grandis to obtain bacterium strain with chloromycetin resistance. The shuttle vector can auto replicate in antiradiation bacterium and colibacillus and may be introduced into antiradiation bacterium. It may be used in various kinds of field environments and possesses luciferase gene.

The invention is applicable to the field of firefly cultivation, and provides a method for restoring habitats and cultivating fireflies and rice. According to the invention, firstly a plot layout is designed, and then a system for restoring habitats and cultivating fireflies and rice is reasonably designed, so that the local ecological environment can be comprehensively and systematically restored, a large amount of farmland water can be saved, and the purposes of recycling the farmland water and realizing zero emission of pollution are achieved. Meanwhile, the fireflies and the rice are co-cultivated, so that the ecological environment restoration degree is inspected, and the fireflies become visual inspection standards of organic healthy rice foods. According to the method, extremely high ecological benefits can be generated through ecological restoration, great social benefits can be brought by restoring the ecological environment, and huge economic benefits can be generated by selling healthy ecological agricultural products and ecological tourism.

The invention relates to firefly luciferase and an encoding gene and an obtaining method thereof. The defect that the original firefly luciferase in North America is poor in heat stability and cannot be used at a high temperature is overcome; and the original firefly luciferase in North America is mutated, so that mutative enzyme of which the heat stability is obviously improved is obtained; and the application value is improved. An amino acid sequence is SEQ ID NO.1; and a protein deoxyribonucleic acid (DNA) sequence in encoding is SEQ ID NO.2. An encoding gene and a preparation method of firefly luciferase mutant in North America are provided. By the method, a recombinant expression vector containing a wild firefly luciferase gene is built; and the protein is purified by using an affinity chromatography method. The method is simple and feasible, simple to operate, and low in cost.

The invention provides an efficiently-expressed high thermal stability luciferase encoding gene and a preparation method of the firefly luciferase. The gene is obtained through chemical synthesis after firefly luciferase genes derived from Photinuspyralis are subjected to molecular modification. At present, the efficiently-expressed high thermal stability luciferase encoding gene has not been reported, the luciferase expressed by the encoding gene in escherichia coli accounts for 72.1%-83.5% of soluble protein in whole cells, and the expression amount of the luciferase is 481-739 mg / 10 g of wet bacteria. According to the efficiently-expressed high thermal stability luciferase encoding gene, the expression product is a luciferase, the thermal stability of the luciferase is high, the temperature can be preserved for 20 minutes under the condition of 40 DEG C, and the relative activity of about 95% can still be kept.

The invention provides an efficiently-expressed high thermal stability luciferase encoding gene and a preparation method of the firefly luciferase. The gene is obtained through chemical synthesis after firefly luciferase genes derived from Photinuspyralis are subjected to molecular modification. At present, the efficiently-expressed high thermal stability luciferase encoding gene has not been reported, the luciferase expressed by the encoding gene in escherichia coli accounts for 72.1%-83.5% of soluble protein in whole cells, and the expression amount of the luciferase is 481-739 mg / 10 g of wet bacteria. According to the efficiently-expressed high thermal stability luciferase encoding gene, the expression product is a luciferase, the thermal stability of the luciferase is high, the temperature can be preserved for 20 minutes under the condition of 40 DEG C, and the relative activity of about 95% can still be kept.

The present invention relates to a luciferase derived from a star-worm belonging to genus Diplocladon, the luciferase inducing luminescence such that a maximum luminous wavelength falls within a range of 557 to 562 nm over the entire pH range of 5.5 to 8.0, or the luciferase inducing luminescence having 1.5 times the luminous intensity of luminescence induced by Photinus pyralis firefly luciferase.

The invention provides a CMP task scheduling method of an improved firefly algorithm. The CMP task scheduling method comprises the following steps: 1, setting the predicted input number N of firefly populations, the light absorption coefficient gamma of a medium, an initial step length factor alpha, the maximum attraction degree beta0 and an attraction degree threshold betaM; 2, initializing the number and positions of firefly populations according to an initialization strategy; 3, calculating the fitness value of the firefly according to the position of the firefly; 4, enabling each firefly to fly to the firefly with the brightness higher than that of the firefly, calculating the fitness value of the firefly after reaching the new position, if the fitness value is superior to the originalposition, the firefly reaches the new position, and otherwise, the firefly stays at the original position; and 5, judging an optimization result, if a termination condition is met, ending an iteration process, otherwise, repeating the operation in the step 3, and iterating the firefly particles again. According to the invention, the convergence speed of the firefly particles is increased, the possibility of falling into a local optimal solution in the optimization process is greatly reduced, the unnecessary iteration frequency is reduced, and the task scheduling completion time is shortened.

The invention discloses a method for embedding a heating pipeline near a root area of a fruit tree. The method comprises the following steps: acquiring the distribution condition of heating temperature fields of the fruit tree root zone under different heating pipeline burying schemes based on computational fluid dynamics simulation, and recording the temperature value of each monitoring point of the fruit tree root zone; and then, carrying out parameter optimization through firefly algorithm iteration, achieving position updating of firefly individuals in each iteration process, and enabling the positions where the firefly individuals are located to represent the distance and depth parameters of heating pipeline burying. For a group of new burying spacing and burying depth parameters, the KNN machine learning model is called to output a corresponding monitoring point temperature value, and then the firefly algorithm is returned to judge whether the monitoring point temperature meets a constraint condition or not; and by taking the distribution uniformity of the heating temperature field of the root zone of the fruit tree as a target, the burying distance and burying depth parameters of the heating pipeline are optimized, and a numerical basis is provided for burying the heating pipeline.

In one embodiment, an object of the present invention is to provide a firefly luciferase having improved thermostability. In one embodiment, the present invention relates to a luciferase mutant having improved thermostability that is a mutant of firefly luciferase comprising an amino acid sequence in which the amino acid residue at the position corresponding to position 393 of SEQ ID NO 1 is substituted, a polynucleotide encoding the luciferase mutant, and a production method of the luciferase mutant.

The invention discloses an artificial wetland system suitable for artificial restoration of aquatic fireflies and a construction method. The artificial wetland system comprises an aquatic area and a terrestrial area which are separated by a water line, wherein the water bottom of the aquatic area and the terrestrial area form a side slope with the gradient smaller than 45 degrees; the water body depth of the aquatic area is not larger than 30 cm, and the average water body flow velocity is not higher than 30 cm / s; and a water level adjusting structure used for adjusting the water depth of the aquatic area is arranged in the terrestrial area and / or the aquatic area. According to the invention, a land and water environment required by survival and reproduction of the aquatic fireflies is constructed, the climbing slope design requirement of the aquatic fireflies is given according to the pupation characteristic of the fireflies, and the water depth and flow velocity of an aquatic area are controlled, so that an ecological environment suitable for survival and reproduction of the firefly larvae is created; and particularly, the water depth of the aquatic area can be controlled by arranging the water level adjusting structure, and the requirements of the aquatic fireflies on the water depth in different seasons can be better met.

The present invention relates to a luciferase derived from a Malaysian Luciola firefly, the luciferase having a maximum luminescent wavelength of 557 nm at pH 8, or the luciferase inducing luminescence having 1.5 times or more the luminous intensity of luminescence induced by Photinus pyralis firefly luciferase.

The invention provides an SSR (Simple Sequence Repeat) molecular marker for a thoracic window, which is characterized in that the SSR molecular marker comprises one or more of 13 groups of SSR molecular markers, and the serial numbers of the 13 groups of SSR molecular markers are SSR308, SSR311, SSR313, SSR319, SSR320, SSR323, SSR324, SSR325, SSR405, SSR406, SSR501, SSR503 and SSR504 respectively; the nucleotide sequences of the 13 groups of SSR molecular marker sites are as shown in SEQ ID NO. 1 to 13. According to the SSR molecular marker disclosed by the invention, a technical system is established, so that the blank of the specific molecular marker of the scirpus juncoides is filled, the SSR molecular marker can be used for identifying and analyzing firefly offspring paternal right, genetic diversity, population genetics and germplasm, and a result can be used for sex selection and evolution research of a scirpus juncoides insect; and evaluating artificial feeding, rejuvenation and protection of the scirpus juncoides.

A three-phase current-based fault diagnosis method for AC asynchronous motor rotor broken bars, synchronously collecting the three-phase current signals of the AC asynchronous motor stator; setting the number K of VMD decomposition according to the signal characteristics; changing the fixed moving steps of fireflies in the basic firefly algorithm GSO Long, based on the initial position of fireflies initialized by chaotic sequences, an improved chaotic variable step-size firefly algorithm CSVGSO was proposed, and the fitness function for analyzing current signals was determined; the VMD decomposition penalty parameter α was optimized by using CSVGSO; based on the global optimal fitness value corresponding The penalty parameter α and the decomposition number K perform VMD decomposition on the stator three-phase current to extract the modal component corresponding to the fundamental frequency of the current signal; determine the reference phase of the current signal based on Park transformation; substitute the obtained phase information into the LMS algorithm to extract the current The basic frequency component in the signal is adaptively filtered; the frequency spectrum analysis is performed on the filtered current signal to detect the fault characteristic frequency of the broken bar of the motor rotor; the invention has the advantages of high accuracy and strong robustness.

The present invention relates to a luciferase derived from a Malaysian Luciola firefly, the luciferase having a maximum luminescent wavelength of 557 nm at pH 8, or the luciferase inducing luminescence having 1.5 times or more the luminous intensity of luminescence induced by Photinus pyralis firefly luciferase.

The invention is applicable to the field of insect pupating equipment, and provides firefly pupating equipment and a pupating method. The equipment comprises a pupation frame, a water collecting tankand a water storage tank, wherein a plurality of pupation boxes are forward obliquely placed on the pupation frame; an overflow port is formed in the front wall of each pupation box and gauze is mounted on each overflow port; a water receiving tank located directly below the overflow ports of the pupation boxes is arranged in the pupation frame; a water pipe bracket is upwardly arranged on the pupation frame; a water supply pipe is mounted on the water pipe bracket; a plurality of water taps are arranged on the water supply pipe; a booster pump is mounted between the water storage tank and thewater supply pipe. By adopting the equipment, large-scale pupation can be realized, the number of modular pupating units can be freely combined, the length and height can be adjusted according to thesize of the space, and the equipment is convenient and simple to operate.

The invention discloses an extraction method and application of firefly chitosan, and further provides a fodder. The extraction process of the firefly chitosan comprises the steps of cleaning and drying fresh fireflies, then decalcifying, removing protein and decoloring, and finally, extracting through an ultrasonic technology matched with the solvent under the aid of alkali, so as to obtain the chitosan. In the extraction process of the firefly chitosan, decalcification and deacetylation help reduce product impurities effectively, the ultrasonic technology cooperates with the solvent so as tofurther promote deacetylation, so that the product quality is higher, and the product can improve the immunity when being used in the fodder and can prohibit bacteria activity,.

The invention discloses a firefly algorithm-based load model parameter determination method and apparatus. The method comprises the steps of establishing an objective function of parameters of a powerload model; calculating a target function value of each firefly individual in the firefly population; calculating the maximum brightness of each firefly individual according to the target function value of each firefly individual; according to the maximum brightness of each firefly individual, determining a firefly king, a preparatory firefly king and a common individual in the firefly population; and determining target parameters of the power load model according to the firefly king, the prepared firefly king and the common individuals. Thus, through the firefly king and the preparatory firefly king, the accuracy of the calculation result of the power load model can be improved, meanwhile, the stability of the output result in the later iteration period can be ensured, and the accuracy of parameter identification can be effectively improved.

According to the invention, a compound for detecting tyrosinase is designed by utilizing catalytic oxidation characteristics of luminescent substance firefly Luciferrin with good stability and excellent biocompatibility in a biological system and tyrosinase in a firefly body, a firefly luciferin derivative is used as a parent structure and o-diphenolic hydroxyl is used as a response group, and thetwo are covalently bonded through an alkyl chain. The compound has good biocompatibility and fluorescence performance, the maximum emission peak is located at 535 nm, and after Tyr is dropwise added,fluorescence is rapidly reduced within 2 min. An action mechanism is that a photo-induced electron transfer process (PET) exists between a o-diphenolic hydroxyl group and a fluorescein structure, after the Tyr is added into the system, the o-diphenolic hydroxyl is oxidized into a quinone structure, the photo-induced electron transfer process is inhibited after electron cloud arrangement in the system is changed, and fluorescence is quenched. A detection limit of 0.06087 M (PBS) is calculated according to a dose-dependent fluorescence spectrum change of FluotyLu. The compound is found to havethe value of detecting Fe<3+> in the interference capacity of metal ions to FluotyLu for detecting Tyr.

The invention provides luciferase, which is derived from Lucidium leucicola, has a protein characteristic of luminous wavelength change under different pH conditions, is orange-yellow light under an acidic condition and bright-yellow light under an alkaline condition, and has an amino acid sequence of SEQ ID NO.1; the luciferase is applied to the firefly luminescence science popularization education kit, so that the common public can extract a luminescence substrate (fluorescein) in a firefly body through operation by himself / herself, carry out a firefly luminescence experiment and understand the principle of firefly luminescence.

In one embodiment, an object of the present invention is to provide a firefly luciferase having improved thermostability. In one embodiment, the present invention relates to a luciferase mutant having improved thermostability that is a mutant of firefly luciferase comprising an amino acid sequence in which the amino acid residue at the position corresponding to position 393 of SEQ ID NO 1 is substituted, a polynucleotide encoding the luciferase mutant, and a production method of the luciferase mutant.