37 results about "Photinus pyralis" patented technology

This invention relates to: the development of a mutant firefly luciferase in order to use dATP as a DNA polymerase substrate upon pyrosequencing, such luciferase being subjected to substrate specificity modification in a manner such that the dATP-induced activity alone is decreased while the ATP-induced activity is maintained; and a mutant firefly luciferase for which the proportion of activity induced by dATP to activity induced by ATP (dATP / ATP) is lower than that for the wild-type firefly luciferase, in which an amino acid identified based on homology analysis as corresponding with the 421st amino acid (glycine) of the amino acid sequence of the wild-type North American firefly (Photinus pyralis) luciferase has been substituted with a polar amino acid.

There is presented a detailed electronic method for simulation of a firefly, other insects, or organisms producing bioluminescence and with light radiation functions similar to a real firefly, other insects, or organisms producing bioluminescence. An illuminated artificial firefly, other insects, or organisms producing bioluminescence can be placed on trees and shrubs in landscape, or at home to produce radiation, at night time, and blinking a gradually decaying light. More particularly it relates to a decorative type of device in the form of a firefly, other insects, or organisms producing bioluminescence and with light radiation functions similar to a real firefly, other insects, or organisms producing bioluminescence. The light is simulated by an electronics program that is a part of compact device with the form of a real firefly, other insects, or organisms producing bioluminescence. Short radiation pulses of microsecond-millisecond duration time in violet and ultraviolet range of radiation wavelengths can be utilized for scaring birds or other animals from undesirable or dangerous places of human activity, such as airports, contaminated ponds, wind turbines, electorized fences and antennas.

A firefly larva breeding device comprises a breeding box, an adjusting mechanism, a feeding mechanism and a humidifying mechanism, the top of the breeding box is rotationally connected with a cover plate through a hinge, a storage groove and a driving groove are formed in the upper half portion and the lower half portion of the breeding box respectively, and the adaptive adjusting mechanism is arranged at the bottom of the inner circumference of the storage groove; a plurality of limiting grooves are formed in the side, away from the adjusting mechanism, of the lower half portion of the storage groove, feeding mechanisms matched with the adjusting mechanism are arranged in the limiting grooves, a turnover cover is rotationally connected to the side, close to the limiting grooves, of the breeding box, and the humidifying mechanism penetrating through the breeding box and connected with the bottom of the cover plate is arranged in the driving groove. A blow-off pipe and a water supply pipe connected with the humidifying mechanism are mounted on one side of the lower half part of each of the storage tank and the driving tank through fasteners, and a baffle is mounted on one side, close to the driving tank, of the breeding box. The firefly larva breeding device can be adjusted according to the needed breeding environment, and meanwhile feeding is convenient.

The invention relates to firefly luciferase and an encoding gene and an obtaining method thereof. The defect that the original firefly luciferase in North America is poor in heat stability and cannot be used at a high temperature is overcome; and the original firefly luciferase in North America is mutated, so that mutative enzyme of which the heat stability is obviously improved is obtained; and the application value is improved. An amino acid sequence is SEQ ID NO.1; and a protein deoxyribonucleic acid (DNA) sequence in encoding is SEQ ID NO.2. An encoding gene and a preparation method of firefly luciferase mutant in North America are provided. By the method, a recombinant expression vector containing a wild firefly luciferase gene is built; and the protein is purified by using an affinity chromatography method. The method is simple and feasible, simple to operate, and low in cost.

The invention provides an efficiently-expressed high thermal stability luciferase encoding gene and a preparation method of the firefly luciferase. The gene is obtained through chemical synthesis after firefly luciferase genes derived from Photinuspyralis are subjected to molecular modification. At present, the efficiently-expressed high thermal stability luciferase encoding gene has not been reported, the luciferase expressed by the encoding gene in escherichia coli accounts for 72.1%-83.5% of soluble protein in whole cells, and the expression amount of the luciferase is 481-739 mg / 10 g of wet bacteria. According to the efficiently-expressed high thermal stability luciferase encoding gene, the expression product is a luciferase, the thermal stability of the luciferase is high, the temperature can be preserved for 20 minutes under the condition of 40 DEG C, and the relative activity of about 95% can still be kept.

The invention provides a CMP task scheduling method of an improved firefly algorithm. The CMP task scheduling method comprises the following steps: 1, setting the predicted input number N of firefly populations, the light absorption coefficient gamma of a medium, an initial step length factor alpha, the maximum attraction degree beta0 and an attraction degree threshold betaM; 2, initializing the number and positions of firefly populations according to an initialization strategy; 3, calculating the fitness value of the firefly according to the position of the firefly; 4, enabling each firefly to fly to the firefly with the brightness higher than that of the firefly, calculating the fitness value of the firefly after reaching the new position, if the fitness value is superior to the originalposition, the firefly reaches the new position, and otherwise, the firefly stays at the original position; and 5, judging an optimization result, if a termination condition is met, ending an iteration process, otherwise, repeating the operation in the step 3, and iterating the firefly particles again. According to the invention, the convergence speed of the firefly particles is increased, the possibility of falling into a local optimal solution in the optimization process is greatly reduced, the unnecessary iteration frequency is reduced, and the task scheduling completion time is shortened.

The present invention relates to a luciferase derived from a Malaysian Luciola firefly, the luciferase having a maximum luminescent wavelength of 557 nm at pH 8, or the luciferase inducing luminescence having 1.5 times or more the luminous intensity of luminescence induced by Photinus pyralis firefly luciferase.

A three-phase current-based fault diagnosis method for AC asynchronous motor rotor broken bars, synchronously collecting the three-phase current signals of the AC asynchronous motor stator; setting the number K of VMD decomposition according to the signal characteristics; changing the fixed moving steps of fireflies in the basic firefly algorithm GSO Long, based on the initial position of fireflies initialized by chaotic sequences, an improved chaotic variable step-size firefly algorithm CSVGSO was proposed, and the fitness function for analyzing current signals was determined; the VMD decomposition penalty parameter α was optimized by using CSVGSO; based on the global optimal fitness value corresponding The penalty parameter α and the decomposition number K perform VMD decomposition on the stator three-phase current to extract the modal component corresponding to the fundamental frequency of the current signal; determine the reference phase of the current signal based on Park transformation; substitute the obtained phase information into the LMS algorithm to extract the current The basic frequency component in the signal is adaptively filtered; the frequency spectrum analysis is performed on the filtered current signal to detect the fault characteristic frequency of the broken bar of the motor rotor; the invention has the advantages of high accuracy and strong robustness.

The invention is applicable to the field of insect pupating equipment, and provides firefly pupating equipment and a pupating method. The equipment comprises a pupation frame, a water collecting tankand a water storage tank, wherein a plurality of pupation boxes are forward obliquely placed on the pupation frame; an overflow port is formed in the front wall of each pupation box and gauze is mounted on each overflow port; a water receiving tank located directly below the overflow ports of the pupation boxes is arranged in the pupation frame; a water pipe bracket is upwardly arranged on the pupation frame; a water supply pipe is mounted on the water pipe bracket; a plurality of water taps are arranged on the water supply pipe; a booster pump is mounted between the water storage tank and thewater supply pipe. By adopting the equipment, large-scale pupation can be realized, the number of modular pupating units can be freely combined, the length and height can be adjusted according to thesize of the space, and the equipment is convenient and simple to operate.

This application discloses a multi-level particle filter algorithm based on the improved firefly step size factor. The method modifies the brightness formula and position update formula in the firefly particle filter algorithm, and uses the adaptive step size firefly algorithm for iterative update, and The result is multi-stage filtered. This application uses the adaptive step size firefly algorithm to drive particles with small weights to move to high likelihood areas, which reduces the problem of particle degradation, achieves higher filtering accuracy with fewer particles, and saves computing resources.

The invention provides luciferase, which is derived from Lucidium leucicola, has a protein characteristic of luminous wavelength change under different pH conditions, is orange-yellow light under an acidic condition and bright-yellow light under an alkaline condition, and has an amino acid sequence of SEQ ID NO.1; the luciferase is applied to the firefly luminescence science popularization education kit, so that the common public can extract a luminescence substrate (fluorescein) in a firefly body through operation by himself / herself, carry out a firefly luminescence experiment and understand the principle of firefly luminescence.

This invention relates to: the development of a mutant firefly luciferase in order to use dATP as a DNA polymerase substrate upon pyrosequencing, such luciferase being subjected to substrate specificity modification in a manner such that the dATP-induced activity alone is decreased while the ATP-induced activity is maintained; and a mutant firefly luciferase for which the proportion of activity induced by dATP to activity induced by ATP (dATP / ATP) is lower than that for the wild-type firefly luciferase, in which an amino acid identified based on homology analysis as corresponding with the 421st amino acid (glycine) of the amino acid sequence of the wild-type North American firefly (Photinus pyralis) luciferase has been substituted with a polar amino acid.

In one embodiment, an object of the present invention is to provide a firefly luciferase having improved thermostability. In one embodiment, the present invention relates to a luciferase mutant having improved thermostability that is a mutant of firefly luciferase comprising an amino acid sequence in which the amino acid residue at the position corresponding to position 393 of SEQ ID NO 1 is substituted, a polynucleotide encoding the luciferase mutant, and a production method of the luciferase mutant.

The present invention relates to a luciferase derived from a star-worm belonging to genus Diplocladon, the luciferase inducing luminescence such that a maximum luminous wavelength falls within a range of 557 to 562 nm over the entire pH range of 5.5 to 8.0, or the luciferase inducing luminescence having 1.5 times the luminous intensity of luminescence induced by Photinus pyralis firefly luciferase.