38 results about "Photinus pyralis" patented technology

The invention provides an efficiently-expressed high thermal stability luciferase encoding gene and a preparation method of the firefly luciferase. The gene is obtained through chemical synthesis after firefly luciferase genes derived from Photinuspyralis are subjected to molecular modification. At present, the efficiently-expressed high thermal stability luciferase encoding gene has not been reported, the luciferase expressed by the encoding gene in escherichia coli accounts for 72.1%-83.5% of soluble protein in whole cells, and the expression amount of the luciferase is 481-739 mg / 10 g of wet bacteria. According to the efficiently-expressed high thermal stability luciferase encoding gene, the expression product is a luciferase, the thermal stability of the luciferase is high, the temperature can be preserved for 20 minutes under the condition of 40 DEG C, and the relative activity of about 95% can still be kept.

The present invention relates to a luciferase derived from a Malaysian Luciola firefly, the luciferase having a maximum luminescent wavelength of 557 nm at pH 8, or the luciferase inducing luminescence having 1.5 times or more the luminous intensity of luminescence induced by Photinus pyralis firefly luciferase.

A three-phase current-based fault diagnosis method for AC asynchronous motor rotor broken bars, synchronously collecting the three-phase current signals of the AC asynchronous motor stator; setting the number K of VMD decomposition according to the signal characteristics; changing the fixed moving steps of fireflies in the basic firefly algorithm GSO Long, based on the initial position of fireflies initialized by chaotic sequences, an improved chaotic variable step-size firefly algorithm CSVGSO was proposed, and the fitness function for analyzing current signals was determined; the VMD decomposition penalty parameter α was optimized by using CSVGSO; based on the global optimal fitness value corresponding The penalty parameter α and the decomposition number K perform VMD decomposition on the stator three-phase current to extract the modal component corresponding to the fundamental frequency of the current signal; determine the reference phase of the current signal based on Park transformation; substitute the obtained phase information into the LMS algorithm to extract the current The basic frequency component in the signal is adaptively filtered; the frequency spectrum analysis is performed on the filtered current signal to detect the fault characteristic frequency of the broken bar of the motor rotor; the invention has the advantages of high accuracy and strong robustness.

This application discloses a multi-level particle filter algorithm based on the improved firefly step size factor. The method modifies the brightness formula and position update formula in the firefly particle filter algorithm, and uses the adaptive step size firefly algorithm for iterative update, and The result is multi-stage filtered. This application uses the adaptive step size firefly algorithm to drive particles with small weights to move to high likelihood areas, which reduces the problem of particle degradation, achieves higher filtering accuracy with fewer particles, and saves computing resources.

This invention relates to: the development of a mutant firefly luciferase in order to use dATP as a DNA polymerase substrate upon pyrosequencing, such luciferase being subjected to substrate specificity modification in a manner such that the dATP-induced activity alone is decreased while the ATP-induced activity is maintained; and a mutant firefly luciferase for which the proportion of activity induced by dATP to activity induced by ATP (dATP / ATP) is lower than that for the wild-type firefly luciferase, in which an amino acid identified based on homology analysis as corresponding with the 421st amino acid (glycine) of the amino acid sequence of the wild-type North American firefly (Photinus pyralis) luciferase has been substituted with a polar amino acid.

In one embodiment, an object of the present invention is to provide a firefly luciferase having improved thermostability. In one embodiment, the present invention relates to a luciferase mutant having improved thermostability that is a mutant of firefly luciferase comprising an amino acid sequence in which the amino acid residue at the position corresponding to position 393 of SEQ ID NO 1 is substituted, a polynucleotide encoding the luciferase mutant, and a production method of the luciferase mutant.