3 results about "Photinus pyralis" patented technology

The invention discloses a firefly luciferase Fluc mutant and application of the firefly luciferase Fluc mutant, wild firefly luciferase Fluc from North America fireflies is taken as a research object, single mutation or combined mutation is carried out on a protein sequence of the firefly luciferase Fluc to obtain a mutant protein, and the mutant protein is used for preparing the firefly luciferase Fluc mutant. Compared with wild type Fluc, the heat stability and activity of the Fluc mutant protein are improved to a certain degree, compared with the wild type Fluc, the heat stability of the preferable mutant protein is improved by 13.2-14.5 DEG C, the activity is improved by 1.4-2.5 times, the yield is improved by 2.4-3.9 times, the purity of the obtained preferable mutant protein is higher than 99%, and it can be seen that the preferable mutant protein can be applied to the field of industrial production. The luciferase Fluc mutant protein provided by the invention has higher enzyme activity, better thermal stability and higher yield, widens the application conditions of luciferase Fluc, has higher practical application value, and is more suitable for large-scale production and industrial use.