MBR membrane pollution control method based on microbial population induction quenching

Abstract

The invention discloses an MBR membrane pollution control method based on microbial quorum sensing quenching, which comprises the following steps: S1, using an MBR reactor for reaction, S2, inoculating activated sludge into 20mL of M9 culture medium taking GCL as a single carbon source at 1% v / v, culturing for 5-7 days in a shaker at 30 DEG C and 150rpm, S3, cleaning GCL-enriched bacteria or the activated sludge with 50mmol / L TRIS buffer salt solution with pH of 7.0, and drying to obtain the MBR membrane pollution control method based on microbial quorum sensing quenching. The method comprises the following steps: adding 100 [mu] L of pure water into an isometric 400 nmol / L AHL as a blank control, boiling the activated sludge for 10 min, adjusting OD600 to 1.0, culturing the mixed solution in a shaking table at 30 DEG C and 120 rpm, taking out one part of the mixed solution at regular time, placing the mixed solution in a water bath at 99 DEG C for 10 min to inactivate bacteria, and carrying out freeze drying to obtain the activated sludge. According to the MBR membrane pollution control method based on microbial population induction quenching, cells adhered to a biological membrane on the surface of the membrane and polysaccharide substances secreted by components are reduced after reaction, and it is proved that along with increase of the sludge retention time, biological pollution in the MBR is lighter.

Description

technical field [0001] The invention relates to the field of pollution control of MBR membranes, in particular to an MBR membrane pollution control method based on microbial quorum sensing quenching. Background technique [0002] The process of membrane bioreactor has experienced traces from maturity to popularization and application, but the problem of membrane fouling has been difficult to solve and hinders the development of this technology. In the field of microbial research, the concept of microbial quorum sensing is almost the same as that of membrane bioreactor technology. It was proposed at the same time and experienced rapid development at the same time. Microbial quorum sensing means that bacteria sense the density of bacterial populations by secreting signal molecules and detecting their concentrations, thereby triggering a series of group behaviors. Previously, research on quorum sensing mainly focused on microbial In recent years, some reports on quorum sensing ...

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Problems solved by technology

[0002] The process of membrane bioreactor has experienced traces from maturity to popularization and application, but the problem of membrane fouling has been difficult to solve and hinders the development of this technology. In the field of microbial research, the concept of microbial quorum sensing is almost the same as that of membrane bioreactor technology. It was proposed at the same time and experienced rapid development at the same time. Microbial quorum sensing means that bacteria sense the density of bacterial populations by secreting signal molecules and detecting their concentrations, thereby triggering a series of group behaviors. Previously, research on quorum sensing mainly focused on microbial In recent years, some reports on quorum sensing in water treatment processes have also appeared one after another, and some studies have achieved good membrane fouling control effects by inhibiting microbial quorum sensing in MBR, which provides a new idea for membrane fouling control, but The research on quorum sensing and its quenching in membrane bioreactors is still in the exploratory stage. The influence of operating parameters of membrane bioreactors, such as sludge residence time, on quorum sensing in reactors has not been explored in depth. Quorum sensing quenching measures are too complex and their stability needs to be strengthened. In addition, the influence of quorum sensing quenching-based membrane fouling control measures on the decontamination efficiency of MBR has not been carefully investigated.