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Microscope image intelligent splicing method and device, medium and equipment

A microscope and image quality technology, applied in image enhancement, image analysis, image communication, etc., can solve the problems of increasing system cost, increasing calculation amount, and calculation complexity, etc., and achieve the effect of improving splicing speed, efficient image acquisition, and high quality

Pending Publication Date: 2022-05-10
MOTIC CHINA GRP CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method must use an automatic stage to obtain image displacement, which increases the system cost and has poor adaptability. At the same time, its image matching based on Fourier transform is complex and increases the amount of calculation.

Method used

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  • Microscope image intelligent splicing method and device, medium and equipment
  • Microscope image intelligent splicing method and device, medium and equipment
  • Microscope image intelligent splicing method and device, medium and equipment

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0065] Please refer to figure 1 As shown, the present invention discloses a method for intelligent stitching of microscope images, which includes 4 core steps according to the flow chart:

[0066] S1. Image acquisition: During the moving process of the stage, the rolling shutter of the camera keeps exposing continuously, and the processor acquires the real-time dynamic image of the exposure;

[0067] S2, detection of image motion blur: convert the image, perform edge detection on the image through the Laplace edge detection algorithm, determine whether the image quality meets the preset evaluation criteria, if so, enter S3, otherwise return to S1;

[0068] S3. Image processing and matching: extracting the first image and the second image, the first image is the currently acquired dynamic image, and the second image is extracted from the same position of the current dynamic image from the same position of the spliced ​​large image image;

[0069] S4. Automatic stitching of do...

Embodiment 2

[0132] Another object of the present invention is to provide an intelligent stitching device for microscope images, which includes a microscope, a camera and computer equipment.

[0133] Wherein, the microscope has a stage and a focusing unit that can move along the X / Y axis. The stage may be an automatic stage or a manual stage, and the focusing unit may be a manual focus knob or an automatic focusing unit, which is not specifically limited in this application.

[0134] The camera is an electronic rolling shutter camera, and a popular digital camera can be used.

[0135] Computer equipment, the computer equipment has a processor and a human-computer interaction unit, which can be a desktop computer, a notebook computer or an intelligent mobile device. The processor is connected to the camera in such a way as to execute the intelligent mosaic method for microscope images as described in Embodiment 1; the interactive interface of the human-computer interaction unit displays dy...

Embodiment 3

[0138] An embodiment of the present application also provides a computer-readable storage medium, at least one instruction or at least one program is stored in the storage medium, and the at least one instruction or at least one program is loaded and executed by a processor to implement the following: The intelligent mosaic method for microscope images described in Example 1 above.

[0139] Those of ordinary skill in the art can understand that all or part of the processes in the methods of the above embodiments can be implemented through computer programs to instruct related hardware, and the computer programs can be stored in a non-volatile computer-readable memory In the medium, when the computer program is executed, it may include the processes of the embodiments of the above-mentioned methods. Wherein, any references to memory, storage, database or other media provided in the present application and used in the embodiments may include non-volatile and / or volatile memory. ...

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PUM

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Abstract

The invention discloses a microscope image intelligent splicing method and device obtained based on a rolling shutter, a medium and equipment. The method discloses a microscope image intelligent splicing method based on an electronic rolling shutter, and the method comprises the steps: proposing an automatic splicing process in the moving process of a microscope objective table based on the camera characteristics of the electronic rolling shutter; carrying out real-time detection on image motion blur; various states in the splicing process are calculated and dynamically displayed, and the moving speed of an objective table is coordinated to perform effective image splicing; the invention discloses a double-projection automatic splicing algorithm. According to the invention, the image of the electronic rolling shutter camera in the moving process of the objective table is intelligently discriminated and automatically spliced, the use is simple, the operation is convenient, and the application range of the electronic rolling shutter camera is effectively expanded; therefore, one conventional microscope and one popular digital camera can complete the function of scanning a digital slice by one digital slice scanner.

Description

technical field [0001] The invention relates to the technical field of optical microscope image processing, in particular to a method, device, medium and equipment for intelligent stitching of microscope images based on rolling shutter acquisition. Background technique [0002] Conventional digital cameras all use shutter exposure imaging, and the effective exposure time is controlled by the shutter to control the photosensitive chip. The exposure imaging of digital camera chips is mainly divided into two methods, global shutter exposure and rolling shutter exposure. The global shutter is realized by exposing the entire scene at the same time. The exposure is turned on and off for each digital pixel of the camera at the same time, and then the value of each pixel of the image sensor is read out to form a complete image. The exposure of the global shutter The advantage is that all pixels are exposed at the same time, and the exposure of each pixel is uniform and stable witho...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G06T5/50G06T3/00G06T3/40G06T7/13G06T7/136H04N5/235H04N5/265
CPCG06T5/50G06T3/4038G06T7/13G06T7/136H04N5/265G06T2207/10056G06T2207/30168H04N23/73G06T3/14
Inventor 栗远黄炳根陈进
Owner MOTIC CHINA GRP CO LTD
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