Method for batch induction of turbot triploids by hydrostatic pressure method

A turbot and triploid technology, which can be used in the preservation of human or animal bodies, fish farming, climate change adaptation, etc. The effect of increasing the induction rate

Active Publication Date: 2022-05-31
YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0007] The present invention discloses a method for inducing turbot triploids in batches by hydrostatic pressure, that is, establishing a technical method for doubling the chromosome set of fertilized eggs to obtain turbot triploids by hydrostati

Method used

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  • Method for batch induction of turbot triploids by hydrostatic pressure method
  • Method for batch induction of turbot triploids by hydrostatic pressure method
  • Method for batch induction of turbot triploids by hydrostatic pressure method

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Effect test

Embodiment 1

[0030] 1. Determination of the dose of oxytocin for female turbot and its effect

[0031] Select female turbot with good gonad development under light and temperature regulation, broodstock breeding water temperature 13.0±0.5°C, light-dark cycle 16h:8h, female fish at 3 years old, body weight 3.0-4.5kg, and inject chorionic gonadotropin respectively according to body weight Hormone (HCG) 100, 250, 500 U / kg, luteinizing hormone releasing hormone A2 (LHRH-A2) 5, 10, 15 μg / kg, a total of 9 groups of experiments, each group was injected with 3 female fish, marked with T , according to the ovulation time and ovulation amount to determine the dose of oxytocin.

[0032] The results are shown in Table 1. Hormone-induced production can significantly promote ovulation time and increase ovulation volume. Among them, the combination of HCG500U / kg and LHRH-A2 10μg / kg has a certain ovulation time and a large ovulation volume, which shows that this dose induces egg production. Synchronizatio...

Embodiment 2

[0054] Batch induction of embodiment 2 turbot triploid

[0055] In this embodiment, the fertilization water temperature, hydrostatic pressure treatment water temperature and hatching water temperature are all strictly controlled at 14.5±0.5° C., and the seawater used is fresh seawater after ultraviolet disinfection and sand filtration.

[0056] Four non-ovulated turbot females with well-developed gonads were selected and injected with oxytocic agents chorionic gonadotropin (HCG) and luteinizing hormone-releasing hormone A2 (LHRH-A2). The injection dose was HCG: 500 U / kg; LHRH- A2: 10 μg / kg, after 48 hours, the artificially squeezed abdomen method was used to collect eggs. Before artificially squeezing the eggs, wipe the body surface and the water and dirt around the genital pores with a dry towel, and obtain unfertilized eggs, which were observed under a microscope. The eggs were transparent and oily. A total of 800 mL of high-quality unfertilized eggs of turbot were obtained ...

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Abstract

The invention relates to a method for batch induction of turbot triploids by a hydrostatic pressure method, which belongs to the technical field of aquatic genetic breeding, and comprises the following steps: (1) collection, preservation and artificial fertilization of turbot semen and ova; (2) triploid induction; the method comprises the following steps: selecting female turbot with good gonad development and no ovulation, and obtaining ova by using an oxytocic; the method comprises the following steps: selecting mature male turbots, manually collecting seminal fluid, putting the seminal fluid into a precooled sperm protection solution at 4 DEG C according to a dilution ratio of the seminal fluid to the protection solution of 1: 10, and preserving the seminal fluid in a refrigerator at 4 DEG C in a dark place for later use; the stored seminal fluid and ova are fertilized through an artificial dry method, 4.5 min after fertilization, fertilized ova are put into hydrostatic pressure tanks to be subjected to hydrostatic pressure shock treatment, the hydrostatic pressure is set to be 60 MPa, the treatment duration is 4-6 min, the fertilized ova are taken out after pressure relief, and the fertilized ova are put into seawater to be incubated continuously; by means of the method, turbot triploids can be obtained in batches, and the induction efficiency is 100%.

Description

technical field [0001] The invention belongs to the technical field of aquatic genetics and breeding, and in particular relates to a production method for inducing turbot triploids in batches by a hydrostatic pressure method, that is, inducing turbot triploids in batches by a hydrostatic pressure method. Background technique [0002] Turbot (Scophthalmus maximus) is a worldwide breeding species of flounder and flounder, and its breeding range covers more than a dozen coastal countries in Europe, America and Asia. China is a big country for turbot breeding, with an annual total output of more than 60,000 tons, accounting for more than 80% of the world's total aquaculture output. It is an important cultured fish in the northern coast of my country. [0003] Because of its sterility, triploid turbot grows faster than ordinary diploids and has stronger stress resistance, especially for breeding large-scale commercial fish (>2.0kg). Studies have shown that at least At the age...

Claims

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Application Information

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IPC IPC(8): A01K61/10A01N1/02
CPCA01K61/10A01N1/0226Y02A40/81
Inventor 孟振刘新富贾玉东徐文腾刘滨张邦胤曲江波
Owner YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
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