Method for changing leaf color of aglaonema rosea tissue culture seedlings by utilizing illumination

A technology for tissue culture seedlings and red leaves, applied in horticultural methods, botanical equipment and methods, applications, etc., can solve the problems of unfavorable production and promotion of red-leafed Grass species, poor red leaf color, and reddish leaf color Turn green and other problems, to achieve the effect of rapid large-scale reproduction and high quality

Pending Publication Date: 2022-06-28
广州花卉研究中心
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

With the increase in the yield of red-leaved Sclerosia tissue culture seedlings year by year, the seedlings and finished potted flowers of some red-leaved Sclerosia species are prone to problems such as leaf color fading from red to green, and the degree of redness of leaves is not strong, which is extremely unfavorable. Mass Production and Popularization of Red-leafed Grass Varieties
The main propagation methods of S. red-leaf are ramet propagation, cutting propagation and tissue culture rapid propagation, etc. The two traditional propagation methods of ramet propagation and cutting propagation are easy to be infected by germs due to improper wound treatment, and the propagation rate is correspondingly low; Tissue culture rapid propagation method has increasingly become the main way of supplying Sclerostidium seedlings, but for a long time, the leaf color and redness of Sclerosia seedlings bred by tissue culture and rapid propagation have always been stable to a large extent. It has become a limiting bottleneck for the propagation of rough rib seedlings and the promotion of industrial development

Method used

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  • Method for changing leaf color of aglaonema rosea tissue culture seedlings by utilizing illumination
  • Method for changing leaf color of aglaonema rosea tissue culture seedlings by utilizing illumination
  • Method for changing leaf color of aglaonema rosea tissue culture seedlings by utilizing illumination

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Example 1: Light intensity treatment

[0019] 1. Method

[0020] Treatment C1: 3000lx, treatment C2: 4000lx, illumination time 10h / d, temperature (25±1)℃.

[0021] The stem segments of 'Guanghuahong ribica' were used as explants, and the sterilized explants were proliferated by inducing proliferation by cluster buds. , C2 conditions, the light time is 10h / d, the temperature is 25±1℃, the proliferation culture is 50d, 10 bottles / treatment, the number of repetitions is set to 3 times, and the medium formula for the proliferation and differentiation culture is: MS+KT 0.2mg / L+6-BA 3.0mg / L+IBA 0.02mg / L+sucrose 30g / L+agar 5.5g / L, pH=6.0; measure the plant height, leaf length and leaf width of individual seedlings differentiated by proliferation, each treatment Measure 30 strains; continue to cultivate for 40 days, the rooting medium formula is: MS+NAA0.5mg / L+AC 0.2g / L+sucrose 30g / L+agar 5.5g / L, pH=6.0; observe leaf color, count red spots After 120 days, the culling rate o...

Embodiment 2

[0031] Example 2: Light Quality Treatment Test

[0032] 1. Method

[0033] Set seven processes: D1: white light (LED light); D2: red light (100% R); D3: red light: blue light = 7: 3 (70% R: 30% B); D4: red light: blue light = 5:5 (50%R:50%B); D5: red light: blue light = 3:7 (30% R: 70% B); D6: blue light (100% B); D7: purple light; illumination time is 10h / d, the temperature is 25±1℃.

[0034] The stem segments of 'Guanghuahong ribica' were used as explants, and the sterilized explants were proliferated by inducing proliferation by cluster buds. Cultured under the condition of D7, the light time is 10h / d, the temperature is 25±1℃, the proliferation culture is 50d, 10 bottles / treatment, the repetition times are set to 3 times, and the medium formula of the proliferation and differentiation culture is MS+KT 0.2mg / L +6-BA 3.0mg / L+IBA 0.02mg / L+sucrose 30g / L+agar 5.5g / L, pH=6.0; then continue to grow roots for 40 days, the rooting medium formula is: MS+NAA 0.5mg / L+AC 0.2g / L+su...

Embodiment 3

[0046] A method for utilizing light to change the leaf color of a tissue culture seedling of Rhododendron chinensis, comprising the following steps: using the sterilized stem section of 'Guanghua Rhizoma chinensis' as an explant, inducing cluster buds, and the cluster bud induction medium is: : 1 / 2MS+6-BA 2.0mg / L+sucrose 30g / L+agar 6.0g / L, pH=6.0; the clump buds were inoculated in the proliferation medium, the light intensity was 4000lx, the light time was 10h / d, and the temperature was Cultivated under the condition of 25±1°C, the formula of the proliferation medium is: MS+KT 0.2mg / L+6-BA 3.0mg / L+IBA 0.02mg / L+sucrose 30g / L+agar 5.5g / L, pH = 6.0; single plantlets obtained by proliferation and differentiation were placed in rooting medium, and the formula of rooting medium was: MS+NAA 0.5mg / L+AC 0.2g / L+sucrose 30g / L+agar 5.5g / L, pH=6.0: The cultivation was carried out under the conditions of light intensity of 4000lx, light time of 10h / d, and temperature of 25±1℃; the rooted se...

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Abstract

The invention discloses a method for changing the leaf color of a crassocephalum ramosissimum tissue culture seedling by utilizing illumination. The method comprises the following steps: culturing the tufted buds of the crassogyne lasioides in the proliferation, differentiation and rooting culture stages under the condition that the illumination intensity is 4000 lx or the light quality condition is that the ratio of red light to blue light is 3: 7, and controlling the illumination intensity and the light quality condition in the proliferation, differentiation and rooting culture stages of the tufted buds of the crassogyne lasioides, so that the red brilliant degree of the leaf color of the red-leaf crassogyne lasioides can be effectively kept; the method is beneficial to efficient and rapid large-scale propagation of high-quality aglaonema rosea seedlings.

Description

technical field [0001] The invention belongs to the field of plant seedling production, and in particular relates to a method for changing the leaf color of a tissue culture seedling of Rhododendron chinensis by utilizing light. Background technique [0002] Aglaonema spp. is a perennial herbaceous foliage plant of the genus Aglaonema in the family Araceae. Red-leaf Coleus is one of the more popular color-leaf varieties in China in recent years. It is widely used indoors as a small family potted plant. It is relatively shade-tolerant, has a long viewing period indoors, and can purify the air. And because the leaves have large red patches, thick ribs, and bright colors, which can fill the shortage of indoor and outdoor monotonous colors in the light-flowering season, it is deeply loved by consumers. . With the annual increase in the yield of red-leafed sturdy rib tissue-cultured seedlings, some red-leaf sturdy rib seedlings and finished potted flowers are prone to problems ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00A01G7/04
CPCA01H4/008A01H4/002A01H4/001A01G7/045
Inventor 宿庆连周晓云易懋升顾梦云曾伟达黄明翅牛文霞刘艳艳
Owner 广州花卉研究中心
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