Human intrahepatic bile duct cancer cell strain ICC-X3 and application thereof

Abstract

The invention discloses a human intrahepatic bile duct cancer cell line ICC-X3 and application thereof, and belongs to the field of microbial animal cell lines. The provided intrahepatic cholangiocarcinoma cell line is a human intrahepatic cholangiocarcinoma cell line ICC-X3, and has been preserved in the China Center for Type Culture Collection on February 22, 2022, and the preservation number is CCTCC NO: C202260. The human intrahepatic cholangiocarcinoma cell line ICC-X3 can be used as a cell model for researching the occurrence, development or metastasis mechanism of intrahepatic cholangiocarcinoma. The human intrahepatic cholangiocarcinoma cell line ICC-X3 can also be used for establishing an intrahepatic cholangiocarcinoma animal model. The human intrahepatic cholangiocarcinoma cell line ICC-X3 can be used as a cell model for studying the differentiation mechanism, cell morphology and dysfunction and tumor infiltration and metastasis mechanism of intrahepatic cholangiocarcinoma, guiding clinical comprehensive diagnosis and treatment and the like.

Description

technical field [0001] The invention belongs to the field of microbial animal cell lines, in particular to a human intrahepatic cholangiocarcinoma cell line ICC-X3 and its application. Background technique [0002] Intrahepatic cholangiocarcinoma (ICC) is the second most common type of liver cancer, and its incidence has been increasing worldwide. The tumor originates from biliary epithelial cells, which account for 10% to 20% of all bile duct malignancies; surgery is only possible in the early stage of the disease, and the prognosis is poor for patients with advanced unresectable disease (70% to 90%). The survival time after diagnosis was less than 12 months. Systemic chemotherapy and radiotherapy with gemcitabine alone or in combination with platinum-based chemotherapeutics have limited efficacy in improving long-term survival. [0003] Human tumor cell lines, especially those with complete data and follow-up, are important tools for the study of tumor biology. Since th...

AI Technical Summary

Problems solved by technology

Since the 1980s, scientists have established and identified many cell lines of malignant cholangiocarcinoma, but mainly extrahepatic cholangiocarcinoma, and few reports on cell lines of intrahepatic cholangiocarcinoma

However, as the number of in vitro passages increases, some unique biological characteristics of the cell line will gradually change or disappear, and even produce characteristics that the original cells do not have.

In addition, cell cross-contamination has been detected in many cell lines. The most common sources of contaminating cells are HeLa, T-24, and M14, and research using these cells can also lead to erroneous results.

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