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Preparation method and application of soybean, coffee and cordyceps flower bioactive peptide

A technology of bioactive peptides and coffee worms, which is applied in the fields of application, skin care preparations, drug combinations, etc., can solve the problem of no soybean coffee Cordyceps flower bioactive peptides, etc., achieve great application value and promotion value, enhance and regulate the human body Immune function, energy-enhancing effect

Pending Publication Date: 2022-07-08
YUNNAN WENSHAN KUNQI PHARMA CORP LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] However, in the prior art, there is no method for preparing the bioactive peptide of soybean coffee Cordyceps flower and the application of related soybean coffee Cordyceps flower bioactive peptide. has great limitations

Method used

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Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0021] A preparation method and application of a soybean coffee cordyceps flower bioactive peptide, characterized in that it comprises a soybean coffee cordyceps flower compound peptide, and the soybean coffee cordyceps flower compound peptide includes a soybean coffee cordyceps flower combination peptide A and a soybean coffee cordyceps flower combination peptide B, the soybean coffee cordyceps flower combination peptide A and the soybean coffee cordyceps flower combination peptide B are both composed of soybean, coffee, and cordyceps flower.

[0022] The protein raw materials of soybean, coffee and cordyceps flower in the soybean coffee and cordyceps flower combination peptide A are all prepared by enzymatic hydrolysis with pepsin and trypsin; the protein raw materials of soybean, coffee and cordyceps flower in the soybean coffee cordyceps flower combination peptide B All are made by papain or Protamex complex protease hydrolysis.

[0023] The mass ratios of soybean, coffee ...

Embodiment 1

[0036] Step 1: First, mix the soybeans, coffee, and cordyceps flowers by 45 parts, 20 parts, and 10 parts evenly; separate the soybeans, cordyceps flowers, and coffee into the basic protein solution; separate the basic protein of soybean, cordyceps flowers, and coffee. The solution is divided into two parts, and Alcalase protease is added to the two alkaline protein solutions; when the two alkaline protein solutions are enzymatically inactivated after the enzymatic hydrolysis is completed, the soybean coffee cordyceps flower combination peptide A and the soybean coffee cordyceps flower combination are obtained respectively. Peptide B; the soybean coffee cordyceps flower combination peptide A and the soybean coffee cordyceps flower combination peptide B are mixed and centrifuged to obtain a supernatant, which is the soybean coffee cordyceps flower compound peptide;

[0037] Step 2: The soybean coffee cordyceps flower compound peptide liquid is subjected to reflux ultrafiltration...

Embodiment 2

[0040] Step 1: Take 80 parts of soybean coffee cordyceps flower compound peptide, 2 parts of crystalline fructose, and 2 parts of food additives by weight; wherein the food additives include by weight: 0.5 parts of sweet orange essence, 0.5 parts of bitter-reducing essence, Luo Han Guo sweet 0.4 part of glycoside, 0.05 part of stevioside;

[0041] Step 2: Dissolve the soybean coffee Cordyceps flower compound peptide in water (the mass ratio of material to water is 1:10), adjust the pH of the solution to 8.5 with 1 mol / L NaOH solution, add alkaline protease for enzymatic hydrolysis, and then use 1 mol / L HCl solution Adjust the pH to 7, add neutral protease for enzymatic hydrolysis, the addition amount is 2500U / kg, after the end, the enzyme is deactivated, centrifuged, the supernatant is taken, and the ultrafiltration membrane with a molecular weight cut-off of 1000u is used for ultrafiltration to obtain a filter with a molecular weight of less than 1000u. liquid to obtain soybe...

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PUM

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Abstract

The invention relates to the field of bioactive peptides, in particular to a preparation method and application of a soybean, coffee and cordyceps flower bioactive peptide. Comprising soybean, coffee and cordyceps flower. Comprising the following steps: uniformly mixing protein raw materials of soybeans, coffee and cordyceps flowers in proportion; separating out a basic protein solution from soybeans, cordyceps flowers and coffee; dividing the basic protein solution in which the soybeans, the cordyceps flowers and the coffee are separated into two parts, and adding protease; after the enzymolysis is completed, carrying out enzyme deactivation, and mixing two parts of alkaline protein solutions subjected to enzymolysis; adding composite small molecule ion peptide liquid into the mixed liquid to remove surface impurities, and centrifuging to obtain supernate, namely the soybean, coffee and cordyceps flower composite peptide. The invention aims to provide a preparation method and application of a soybean, coffee and cordyceps flower bioactive peptide which can be used for preparing cosmetics, foods, medicines and health-care products and can enhance and regulate the immune function of a human body and improve the disease resistance of the human body.

Description

technical field [0001] The invention relates to the field of bioactive peptides, in particular to a preparation method and application of a soybean coffee cordyceps flower bioactive peptide. Background technique [0002] Bioactive Peptides (BAP) is a general term for different peptides ranging from dipeptides to complex linear and cyclic structures composed of 20 natural amino acids in proteins with different compositions and arrangements. It is a multifunctional compound derived from proteins. Bioactive peptides have a variety of human metabolism and physiological regulation functions, are easy to digest and absorb, and have the functions of promoting immunity, hormone regulation, antibacterial, antiviral, lowering blood pressure, and lowering blood lipids. They are currently the most popular research topics in the international food industry. Functional factors for development prospects. [0003] However, there is no method for preparing soybean coffee Cordyceps flower bi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06A61K8/64A61Q19/00A61K38/01A61P37/04A61P25/28A23L33/18A23J3/16A23J3/14A23J3/34
CPCC12P21/06A61K8/64A61K8/645A61Q19/00A61K38/011A61K38/01A61P37/04A61P25/28A23L33/18A23J3/16A23J3/14A23J3/346A23J3/34A61K2800/10A23V2002/00A61K2300/00A23V2200/324A23V2250/55A23V2250/284A23V2250/6422A23V2250/606A23V2250/254A23V2250/262Y02A50/30
Inventor 屈浩
Owner YUNNAN WENSHAN KUNQI PHARMA CORP LTD
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