HBV infected tree shrew model construction method and tree shrew model

A construction method and technology of tree shrews, applied in the field of animal model construction, can solve the problem of not being able to completely simulate the pathological process of HBV

Active Publication Date: 2022-07-12
昆明市第一人民医院
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Problems solved by technology

Many studies have found that animal models of hepatitis B such as marmots, ducks, and transgenic mice have relatively large limitations. Can not fully simulate the pathological process of HBV

Method used

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  • HBV infected tree shrew model construction method and tree shrew model
  • HBV infected tree shrew model construction method and tree shrew model
  • HBV infected tree shrew model construction method and tree shrew model

Examples

Experimental program
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Embodiment 1

[0049] A method for constructing a tree shrew model of HBV infection, such as Figure 1-Figure 9 As shown, it specifically includes the following steps:

[0050] First, conduct in vitro experiments, including:

[0051] In step S1, the HepG 2.2.15 cell line, the HepG 2 cell line and the ITh 6.1 cell line are respectively recovered, subcultured and cryopreserved, wherein the HepG 2.2.15 cell line, the HepG 2 cell line and the ITh 6.1 cell line are respectively:

[0052] The method for recovering the cell line specifically includes the following steps:

[0053] Step S11, after taking out the cell line cryopreservation tube from liquid nitrogen, quickly place it in a 37°C water bath for recovery;

[0054] Step S12, immediately after the cell suspension is completely melted, it is divided into separate packages and centrifuged;

[0055] Step S13, removing the supernatant after the centrifugation, washing the cells repeatedly, and repeating the step of centrifuging to remove the ...

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Abstract

The invention relates to an animal model, and discloses an HBV (Hepatitis B Virus) infected tree shrew model construction method which specifically comprises the following steps: S1, respectively carrying out resuscitation, subculture and cryopreservation on cell strains; step S2, preparing an HBV (Hepatitis B Virus) culture solution; s3, carrying out HBV activity evaluation and screening the effective titer of an HBV culture solution; s4, obtaining a culture medium infected with HBV; step S5, screening out effective HBV supernate concentration; s6, setting experiment groups; and S7, constructing an animal model and obtaining an experimental conclusion according to experimental data. According to the invention, by constructing the HBV infected tree shrew model, the problems of relative hysteresis of HBV persistent infection, pathogenic mechanism research and development and evaluation of antiviral drugs due to lack of a stable and effective HBV animal model and the like can be effectively solved, and the HBV infected tree shrew model has great significance in further research of hepatitis B, hepatic fibrosis, liver cirrhosis, liver cancer and other diseases.

Description

technical field [0001] The invention relates to the construction of an animal model, in particular to a method for constructing an HBV-infected tree shrew model and a tree shrew model. Background technique [0002] Hepatitis B virus (HBV) is the causative agent of hepatitis B and belongs to the family Hepadnaviridae. It replicates itself through reverse transcription and is precisely regulated by viral and host factors. HBV infection of liver cells can cause acute and chronic hepatitis, and even severe liver cirrhosis and liver cancer. China is a high-risk area for HBV infection. There are nearly 100 million HBV carriers, of whom 20% to 30% suffer from serious diseases such as liver cirrhosis and liver cancer, which are increasing year by year. The incidence of HCC in China accounts for 55% of the world's, about 90% % for hepatitis B-related liver cancer. The research on the pathogenesis of HBV has already become a research hotspot, but this research is still limited by th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K67/027C12N5/09C12N5/071
CPCA01K67/027C12N5/0693C12N5/067A01K2227/10A01K2267/0337
Inventor 李立胡宗强尹燕锋颜春涛江杰王依婷马丽
Owner 昆明市第一人民医院
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