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Traditional Chinese medicine alpinia oxyphylla EST-SSR marker primer and application thereof

A technology for labeling primers and traditional Chinese medicines, applied in biochemical equipment and methods, microbe determination/inspection, DNA/RNA fragments, etc., can solve the problems of the diversity and content differences of the medicinal ingredients of the nutraceutical fruit

Active Publication Date: 2022-07-22
HAINAN MEDICAL COLLEGE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Summarizing the above studies, it was found that there are significant differences in the diversity and content of medicinal ingredients in the fruits of different origins.

Method used

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  • Traditional Chinese medicine alpinia oxyphylla EST-SSR marker primer and application thereof
  • Traditional Chinese medicine alpinia oxyphylla EST-SSR marker primer and application thereof
  • Traditional Chinese medicine alpinia oxyphylla EST-SSR marker primer and application thereof

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Experimental program
Comparison scheme
Effect test

Embodiment

[0030] Test material:

[0031] Table 1 Information of puzzle samples in different regions

[0032]

[0033]

[0034] 1. Select the tender leaves of 17 regions to extract total DNA, and refer to the extraction method. Steps in the instructions of the plant genomic DNA extraction kit.

[0035] 2, utilize the EST-SSR marker designed by the present invention to analyze, and the concrete steps are as follows:

[0036] 2.1 EST-SSR primer screening and amplification:

[0037]2.1.1 PCR reaction system: The optimal reaction system for this experiment is 20 μl: 10 μl 2×Power Taq PCRMasterMix (ie 10 μl DNA Polymerase, Buffer containing Mg 2+ , 2.0mM / μl dDTP Mixture), 2μl DNA, 2μl primer (concentration 10μmol / L), 6μl ddH 2 O (sterilization).

[0038] 2.1.2 PCR reaction program: pre-denaturation at 94°C for 2min, denaturation at 95°C for 45sec, annealing for 45sec (annealing temperature is the optimum temperature for each primer), extension at 72°C for 10min, 36 cycles, and sto...

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Abstract

The traditional Chinese medicine alpinia oxyphylla EST-SSR marker primer is developed for the first time and comprises 20 pairs of primer sequences, the primer can be used for genetic diversity and clustering analysis of the traditional Chinese medicine alpinia oxyphylla from different region sources, traditional Chinese medicine alpinia oxyphylla germplasm in different regions can be rapidly and accurately distinguished, and a good basis is established for directional breeding, variety standardization and the like of alpinia oxyphylla.

Description

technical field [0001] The invention relates to the technical field of molecular markers, in particular to a traditional Chinese medicine Yizhi EST-SSR marker primer and its application. Background technique [0002] Nourishing Alpinia oxyphylla Miq. is one of the four famous southern medicines in my country, and it is also an important medicinal and edible homologous plant announced by the Chinese Ministry of Health. After long-term artificial cultivation and selection, rich variations have been formed. However, the current research on Yizhi mainly focuses on the fields of chemical components, pharmacology, clinical and cultivation, and there is still a lack of exploration of molecular mechanisms. The benefits that can be retrieved from NCBI The genetic information of Zhizhi is limited to 23 SSRs developed from the genomic DNA of Zhizhi leaves, and its functional gene database is lacking, and the research on molecular biology and metabolic pathways of Zhizhi is almost blank...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6895C12N15/11
CPCC12Q1/6895C12Q2600/156
Inventor 潘坤侯杰刘嘉祺罗喻超
Owner HAINAN MEDICAL COLLEGE