Natural maytansine compound and application thereof in pharmacy
A technology of maytansine derivatives and compounds, which is applied in the field of medicine, and can solve the problems of insufficient research on the chemical components of guacamole
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Embodiment 1
[0015] Example 1 Extraction of the compound of the present invention from Peach tree
[0016] (1) Extraction: pulverize the dried peach tree fruit (10.7 kg), percolate and extract with 95% ethanol at room temperature, concentrate under reduced pressure to obtain an extract, and disperse the extract in water with ethyl acetate. Extraction, recovery of solvent under reduced pressure, and concentration to dryness to obtain 420 g of ethyl acetate extract;
[0017] (2) Separation: 420 g of the ethyl acetate extract was subjected to macroporous resin HP-20 column chromatography, and ethanol-water (30:70→45:55→60:40→75:25→90:10→100 : 0) gradient elution, the amount of each gradient is 50 liters, and the fractions are collected; wherein the macroporous resin ethanol-water 60:40 fraction (43.2 g) is subjected to silica gel column chromatography, and dichloromethane-methanol (30:1 →15:1→10:1→8:1→5:1→2:1→1:1) Gradient elution, the amount of each gradient is 5 liters; the dichloromethane...
Embodiment 2
[0024] Inhibitory effect test of compounds of formula (I) and (II) on the proliferation of tumor cell lines HeLa, MV-4-11, MCF-7, MCF-7 / ADR
[0025] The compounds of formula (I) and (II) were tested by tetramethylazolium salt (MTS) colorimetric method on four tumor cell lines HeLa (cervical adenocarcinoma cell line), MV-4-11 (human myeloid mononuclear cell line) Cell leukemia cell line), MCF-7 (human breast cancer cell line), MCF-7 / ADR (human breast cancer adriamycin-resistant cell line) cell proliferation inhibition (ACS MedicinalChemistry Letters 2018,9,502.);
[0026] at 37°C and 5% CO 2 The cells were subcultured under the following conditions, and the cells in the logarithmic growth phase were selected, the medium was aspirated, and the cells were counted by gently pipetting and counting at 5 × 10 4 The cell density was seeded in a 96-well plate, and 90 μL was seeded in each well, and 10 μL of compound was added. Each compound was set up with a concentration gradient, an...
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