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Droplet-based deformation method for delivering material into cells and chip therefor

A droplet and cell technology, applied in chemical instruments and methods, laboratory containers, laboratory utensils, etc., can solve the problems of cell blocking bottleneck structure, uneven material delivery, and reduced cell survival rate, so as to avoid channel blockage, ensuring economic viability, high efficiency and effective

Pending Publication Date: 2022-08-05
高丽大学校算学协力团
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

In this case, however, there is the problem of cells clogging the bottleneck structure
In addition, the cells are in direct contact with the channel wall to experience stress caused by friction etc., thereby reducing cell viability and channel blockage occurs
In addition, there is a problem that the material delivery is not uniform due to the uneven cell deformation due to the uneven velocity, and the material delivery efficiency is fundamentally low since the delivery occurs only through the phenomenon of diffusion.

Method used

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  • Droplet-based deformation method for delivering material into cells and chip therefor
  • Droplet-based deformation method for delivering material into cells and chip therefor
  • Droplet-based deformation method for delivering material into cells and chip therefor

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[0078] Flow rate comparison

[0079] Figure 9 Photographs taken after the same time period have elapsed for both the prior art and the disclosed experimental conditions are shown.

[0080] refer to Figure 9 , it can be seen that the cell positions are different due to the different flow rates in the prior art. However, it can be seen that the present disclosure shows a constant and uniform flow rate.

[0081] In other words, in the prior art, the flow rate was non-uniform because the cells diffusely flowed widely in the channel. However, the droplets according to the present disclosure are generated at the center of the channel, which means that the droplets flow with a very uniform shape and velocity. This means that in the case of the present disclosure encapsulating cells in the form of droplets, the flow rate is almost unchanged despite the microfluidic behavior. This thus results in homogeneous material delivery within the cell.

[0082] Microtubule blockage c...

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PUM

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Abstract

There is provided a method for delivering a material into a cell, comprising the steps of forming a droplet of the material to be delivered and the cell and subjecting the formed droplet to deformation to deliver the material to be delivered into the cell.

Description

technical field [0001] The present disclosure relates to droplet deformation-based methods and chips therefor for delivering materials into cells, and, more particularly, to improvements in the intracellular delivery of materials achieved through the use of droplets comprising a hydrophilic solution containing cells Droplet deformation-based methods for delivering materials into cells and chips therefor for efficiency, economic feasibility, etc. Background technique [0002] Intracellular delivery of materials is one of the most fundamental experiments in cell engineering. Materials are typically delivered using a carrier or by defining nanopores in the cell membrane / nuclear membrane. In viral or liposome-centric vector technologies, efficient material delivery is possible when the technology is optimized, but there are limitations such as safety, slow delivery, labor / cost-intensive vector preparation, and low reproducibility And other issues. [0003] On the other hand, ...

Claims

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Application Information

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IPC IPC(8): B01L3/00
CPCC12M23/16C12M35/02C12M35/04C12N15/87B01L3/502761B01L3/502784B01L2200/0605B01L2200/0636B01L2200/0647
Inventor 郑峨灆朱炳柱李河成
Owner 高丽大学校算学协力团
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