Droplet-based deformation method for delivering material into cells and chip therefor
A droplet and cell technology, applied in chemical instruments and methods, laboratory containers, laboratory utensils, etc., can solve the problems of cell blocking bottleneck structure, uneven material delivery, and reduced cell survival rate, so as to avoid channel blockage, ensuring economic viability, high efficiency and effective
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[0077] Experimental example
[0078] Flow rate comparison
[0079] Figure 9 Photographs taken after the same time period have elapsed for both the prior art and the disclosed experimental conditions are shown.
[0080] refer to Figure 9 , it can be seen that the cell positions are different due to the different flow rates in the prior art. However, it can be seen that the present disclosure shows a constant and uniform flow rate.
[0081] In other words, in the prior art, the flow rate was non-uniform because the cells diffusely flowed widely in the channel. However, the droplets according to the present disclosure are generated at the center of the channel, which means that the droplets flow with a very uniform shape and velocity. This means that in the case of the present disclosure encapsulating cells in the form of droplets, the flow rate is almost unchanged despite the microfluidic behavior. This thus results in homogeneous material delivery within the cell. ...
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