Preparation of crust oligosaccharide and use

Abstract

The present invention relates to preparation and application of crust oligosaccharide and belongs to the field of deep chitin processing technology. Non-specific enzyme is used to raise the hydrolysis degree of chitosan and the separation and purification of hydrolytic product. The key point of the present invention is that the chitosan material is produced into crust oligosaccharide powder through acid dissolving, heating, enzymolysis and hydrolysis with compoiste, enzyme comprising polysaccharide hydrolase, proteinase and lipase, deactivating enzyme, filtering, ultrafiltering, ion exchange or nanometer filtering, vacuum concentration and drying. The product is used in producing various functional health foods with the functions of immunological regulation and blood fat regulation.

Description

technical field [0001] The invention relates to a preparation method and application of chitooligosaccharides, in particular to a preparation method of chitooligosaccharides using a compound enzyme hydrolysis method and the application of chitooligosaccharides, belonging to the technical field of chitin deep processing. Background technique [0002] Chitooligosaccharides are a class of water-soluble oligosaccharides linked by N-acetyl-D-glucosamine (NAG) and / or D-glucosamine (GA) through β-1,4 glycosidic bonds. Prepared by hydrolyzing chitin or chitosan. Chitin and chitosan can be obtained from the shells of shrimps and crabs, the shells of insects and the cell walls of fungi through acid-base treatment. This is a renewable resource, the total amount of resources on the earth is second only to cellulose. However, compared with cellulose, its structure contains acetamido or free amino groups in addition to hydroxyl groups. Its functional properties and uses are more importa...

AI Technical Summary

Problems solved by technology

There are still some difficulties in applying these non-specific hydrolytic enzymes to industrialized production of chitosan: one is that the degree of hydrolysis of chitosan by non-specific enzymes is limited. The amount of use is large (generally above 20%, and the enzyme-to-substrate ratio reaches 0.2-0.3: 1), on the other hand, the product yield is also low (20%-40%), and the relative molecular mass of the product is too large, usually only up to About 10,000, while the relative molecular mass of chitosan with higher physiological activity is below 5,000; second, because chitosan is insoluble in water, after dissolving in acid solution, the viscosity of the solution is large, and the amount of dissolution is limited. The concentration is only 1-3%, so that the separation and purification of the product and the processing capacity of concentration and drying increase, thereby causing the increase of production cost; the third is that although the separation and purification method of the hydrolyzate has the application of ultrafiltration separation, it is not effective for the acid-dissolved band. The excess acid and excessive hydrolyzate monosaccharides cannot be removed, and the product purity and quality are not high

Its technology is not perfect enough to be applied to industrial production

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