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Automatic liposome preparation apparatus and method for prepaving liplsome by same

A technology of liposomes and lipids, which is applied in the field of medicine, can solve problems such as difficult to encapsulate effective doses of drugs, and achieve the effects of controllable size, uniform particle size, and narrow particle size distribution

Inactive Publication Date: 2005-01-26
XIAN LIBANG PHARMA TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0033] (6), traditional liposome industrial preparation methods and defects
[0041] For many macromolecular compounds, the aforementioned two methods are difficult to encapsulate an effective amount of drugs

Method used

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  • Automatic liposome preparation apparatus and method for prepaving liplsome by same
  • Automatic liposome preparation apparatus and method for prepaving liplsome by same
  • Automatic liposome preparation apparatus and method for prepaving liplsome by same

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0090] Embodiment 1: use automatic liposome preparation device to prepare the liposome of 100nm particle size

[0091] Liposome formulation:

[0092] name

molecular weight

Mole (%)

Feed amount (g)

Lecithin

760

60

18.24

cholesterol

386.7

39

6.03

Vitamin E

472

1

0.19

[0093] Add the prescribed amount of lecithin, cholesterol and vitamin E to 20ml of ethanol, heat to 45°C and stir to dissolve it completely, then add it to the organic liquid storage tank, add 180ml of water for injection to the aqueous solution storage tank, and the extruder has a built-in double-layer 100nm filter membrane. Start the infusion pump, and the ethanol adjuvant solution and 180ml water for injection are pressed into the mixer and the extruder of the new automatic liposome preparation device through high-performance liquid chromatography preparation pumps A and B respectively. Ad...

Embodiment 2

[0095] Embodiment 2: Use the automated liposome preparation device of the present invention to prepare liposomes that can be used for pH gradient method to encapsulate medicines with a particle size of 100nm

[0096] The liposome prepared by the device of the present invention can be used to encapsulate all medicines that can be encapsulated by the traditional pH gradient method, and only the preparation of liposome doxorubicin is used as an example for verification.

[0097] Liposome formulation:

[0098] name

molecular weight

Mole (%)

Feed amount (g)

Lecithin

760

60

18.24

cholesterol

386.7

39

6.03

Vitamin E

472

1

0.19

[0099] Add the prescribed amount of lecithin, cholesterol and vitamin E to 20ml of ethanol, heat to 45°C and stir to dissolve it completely, then add it to the organic liquid storage tank, add 180ml of 400mM, pH4 tartaric acid solution to ...

Embodiment 3

[0101] Embodiment 3: use novel automatic liposome preparation device to prepare fat-soluble drug liposome

[0102] The device of the present invention can be used to prepare all fat-soluble drug liposomes, and only the preparation of liposome miconazole is used as an example for verification.

[0103] Drug liposome prescription:

[0104] name

molecular weight

Mole (%)

Feed amount (g)

Lecithin

760

87

19

cholesterol

386.66

9.2

1.0

Oleic acid

479.15

15.0

2.5

miconazole nitrate

282

15

0.3

Azone

281

0.1

0.01

Vitamin E

472

0.2

0.03

peppermint oil

0.2

0.3

[0105] Add 40ml of ethanol to lecithin, cholesterol, oleic acid, miconazole nitrate, azone, vitamin E and peppermint oil, heat to 45°C and stir to dissolve completely, then add to the organic li...

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Abstract

The present invention relates to an automatic liposome preparation equipment and method for preparing liposome by using said equipment. Said equipment includes 1). high-pressure pump; 2). solution mixer; 3). liposome grain size control device; and 4). high-pressure ultrafiltering dialysis column. Said invention uses the high-pressure pump to make the phospholipid dissolved in organic phase, liposoluble medicine and aqueous solution be quickly mixed in solution mixer to obtain multiphase multilayer liposome, then makes said multiphase multilayer liposome directly pass through liposome extruding device so as to obtain large monochamber liposome, and the medicine which is not wrapped in the liposome and organic solvent can be removed by using high-pressure ultrafiltering dialysis column.

Description

1. Technical field [0001] The invention belongs to the field of medicine and relates to a liposome preparation device, in particular to an automatic liposome preparation device and a liposome preparation method using the device. 2. Background technology [0002] Liposomes (liposomes, also known as lipid globules) are hollow globules composed of phospholipid bilayer membranes. The main components that make up liposomes are phospholipids and other lipid compounds. As early as 1961, British scholars Bangham and Standish discovered that when phospholipid molecules were dispersed in water, they would naturally form orderly arranged multilayered vesicles similar to biomembrane structures. Each lipid layer of the vesicle is composed of ordered phospholipid bimolecules; the center of the vesicle and the layers are separated by water. People call this kind of hollow globule similar to the biomembrane structure of bilayer membrane form liposome afterwards. The properties and partic...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61J3/00A61K9/127
Inventor 王九成惠民权陈涛
Owner XIAN LIBANG PHARMA TECH
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