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Artificial SHIRO method for forming Japan pine mushroom

A matsutake and artificial technology, applied in the field of artificial pine mushrooms, can solve the problems of difficult artificial cultivation of Japanese matsutake, no growth, etc.

Inactive Publication Date: 2005-05-04
THE UNIV OF TOKYO
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, with this attempt, matsutake mycelia did not grow to a practical extent to form sira
Therefore, it is currently considered difficult to artificially cultivate Japanese matsutake mushrooms

Method used

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  • Artificial SHIRO method for forming Japan pine mushroom
  • Artificial SHIRO method for forming Japan pine mushroom

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0048] Culture medium preparation

[0049] The medium for adding the above-mentioned active ingredient (such as a substance capable of controlling cell membrane permeability of matsutake mycelia) is not particularly limited as long as matsutake mycelium can grow therein. Both soil collected from the natural environment and an artificial medium (for example, soil and vermiculite in a ratio of 1:1) can be used as the medium. In Examples of the present invention, black soil containing a loam layer of Kanto Plain was used as a base.

[0050] This medium is preferably sieved through a sieve so that the medium has a small and uniform particle size. The soil particle size is preferably adjusted to about 3 mm or less, more specifically, to about 2-3 mm. In the case of an artificial medium, it is preferable to adjust the grain size to about 2 mm or less, more specifically, to about 1-2 mm.

[0051] The culture medium made uniform in particle size as described above can be dried by...

Embodiment approach

[0058] Preferred embodiments of the method of the present invention will be explained below.

[0059] As described above, it can be induced by culturing matsutake mushroom hyphae in a medium containing the aforementioned active ingredients such as substances capable of controlling the cell membrane permeability of matsutake mushroom hyphae (for example, surfactants and / or natural vegetable oils). Artificial Silao of Japanese matsutake mushrooms. Preferably, an actively growing mycelia inoculum of matsutake mushroom is prepared first, and then the inoculum is cultured in a medium containing the aforementioned active ingredient. The method for preparing an actively growing matsutake mycelial inoculum comprises (1) a step of inducing growth of matsutake mushroom mycelium, and (2) a step of preparing matsutake mushroom mycelial inoculum.

[0060] These two steps are explained below. Both steps are performed aseptically.

[0061] (1) Steps of inducing matsutake mycelium growth ...

Embodiment 1

[0076] Method for forming artificial siraux of matsutake mushrooms using a surfactant (Tween 80).

[0077] (1) Preparation of matsutake mushroom mycelium inoculum

[0078] One piece of hyphae was taken from stock mycelium of matsutake mushroom strain (Tm 89; preserved in the laboratory of the inventor of the present invention), and cultured on Ohta agar medium for 4 weeks. The composition of the Ohta medium is as follows.

[0079] Element

Concentration (per 1000ml)

glucose

citric acid

ammonium tartrate

K H 2 PO 4

MgSO 4 ·7H 2 o

CaCl 2 2H 2 o

HEPES *

Inorganic solution **

vitamin solution ***

pH

10 grams

1 g

1 g

1 g

1 g

50 mg

7 grams

10ml

10ml

Adjust to 5.1 with 1N KOH

[0080] * N-2-Hydroxyethylpiperazine-N'-2-ethanesulfonic acid

[0081] ** Inorganic solution (per 1000ml): FeCl 3 5 mg, MnSO 4 ·4-6H 2 O 50 mg, ZnSO 4 ·7H 2 O 300 mg, CoSO 4 ·7H 2 O 50 mg, CuSO...

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Abstract

The present invention relates to a method for forming artificial Shiro of matsutake mushroom, the method comprising culturing matsutake mushroom hyphae in a medium containing a substance capable of controlling the cell membrane permeability of the hyphae as an active ingredient; the present invention also relates to another A method of forming artificial Shiro of matsutake mushroom, the method comprising culturing matsutake mycelia in a medium containing a substance capable of enhancing the hydrophilic property of the hyphae as an active ingredient.

Description

[0001] Cross References to Related Applications [0002] This application is based on and claims priority from prior Japanese Patent Application No. 2001-018691 filed on January 26, 2001, which is incorporated herein by reference. technical field [0003] The present invention relates to a method of forming artificial Shiro of Tricholoma Matsutake (hereafter referred to as "Matsutake"). Background technique [0004] Generally, as a method of efficiently cultivating a large number of mushrooms, a method of multiplying mycelia of mushrooms in a liquid medium is known. However, for Japanese matsutake mushrooms, since Japanese matsutake mushrooms are ectomycorrhizal fungi, their production requires the roots of living trees. The mycelium of the Japanese matsutake mushrooms in the forest soil where the Japanese red pine grows forms a coexistence relationship with the roots of the American red pine and reproduces. The hyphae of matsutake mushrooms and aggregates ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01G1/04
CPCA01G18/20A01G18/40A01G18/50
Inventor 铃木和夫亚历克西·介朗-朗盖特禄敏·瓦里奥
Owner THE UNIV OF TOKYO