Preparation of 1,2-diacyl-3(6-deoxy-6-sulfo-alpha-D-glucopyranosyl)-sn-glycerin ester

A technology of sulfonic acid group and diacyl group, applied in 1 field, can solve problems such as unsuitable extraction and separation of preparation methods, limited sources of raw materials, etc., and achieve the effect of convenient research and development, rich raw materials and good effect

Inactive Publication Date: 2005-11-09
INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the extraction and separation of 1,2-diacyl-3-(6-deoxy-6-sulfonic acid-α-D-glucopyranosyl)-sn-glycerides are mainly carried out from microalgae such as blue Extracted from green algae, using a silica gel column for multiple separations; the source of raw materials is limited and the preparation method is not suitable for large-scale extraction and separation

Method used

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  • Preparation of 1,2-diacyl-3(6-deoxy-6-sulfo-alpha-D-glucopyranosyl)-sn-glycerin ester
  • Preparation of 1,2-diacyl-3(6-deoxy-6-sulfo-alpha-D-glucopyranosyl)-sn-glycerin ester
  • Preparation of 1,2-diacyl-3(6-deoxy-6-sulfo-alpha-D-glucopyranosyl)-sn-glycerin ester

Examples

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Effect test

Embodiment 1

[0026] 1. Extraction of total lipids from seaweed

[0027] Take 1 kg of fresh Ulva, freeze-dry and pulverize, add chloroform:methanol (1:1) 1L to soak for 24 hours, filter, filter residue and then soak for 10 hours in chloroform:methanol (1:1) 1L for a second time, filter and combine The filtrate was evaporated to dryness with a rotary evaporator under reduced pressure, then dissolved in 200 mL of chloroform-methanol (1: 1), and added 0.88% KCl solution, the final chloroform: methanol: the ratio of water was 1: 1: 0.9, and stood to separate layer, remove impurities, and evaporate the organic phase to dryness under reduced pressure to obtain 15.5 grams of total seaweed lipids;

[0028] 2. Pretreatment and packing of diethylaminoethyl cellulose

[0029] Weigh 15g of diethylaminoethyl cellulose, soak it with 1M HCl and 1M NaOH for 1.5 hours respectively, then wash it with methanol and chloroform which are twice the volume of diethylaminoethyl cellulose, and pump it with a Buchne...

Embodiment 2

[0042] 1. Extraction of total lipids from seaweed

[0043] Take 0.3 kg of dry turpentine algae, crush it, add 1 L of n-hexane-ethanol mixed solvent (1:1 volume ratio) to soak for 24 hours, filter, and soak the filter residue with 1 L of n-hexane-ethanol (1:1) mixed solvent for a second time After 10 hours, filter and combine the filtrates, evaporate to dryness with a rotary evaporator under reduced pressure, then dissolve in 100 mL of n-hexane: ethanol (1:1), and add 0.4% KCl solution, the final ratio of n-hexane: ethanol: water is 1 : 1: 0.6, standing for stratification, removing impurities, and evaporating the organic phase to dryness under reduced pressure to obtain 4.5 grams of total seaweed lipids;

[0044] 2. Pretreatment and packing of diethylaminoethyl cellulose

[0045] Weigh 15g of diethylaminoethyl dextran, soak it with 1M HCl and 1M NaOH for 3 hours, then wash with ethanol and n-hexane that are three times the volume of diethylaminoethyl dextran, and wash with a c...

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Abstract

The preparation of 1,2-diacyl-3-(6-deoxy-6-sulfo- alpha-D-glucopyranosyl)-sn-glycerin ester includes the steps of leaching crushed sea algae with organic solvent, adding water solution of potassium chloride into filtrate to delaminate and depression evaporating organic phase to obtain general algae ester, mounting diethyl amino ethyl cellulose through serial treatment in column; dissolving general algae ester in mixed solvent of chloroform and methanol, depurating in the column with the mixed chloroform and methanol solvent and eluted with mixed solvent of CHCl3, CH3OH and NH3.H2O to obtain coarse product; and purifying with mixed of CHCl3, CH3OH and KCl solution and evaporation to eliminate organic phase; and dissolving the product in methanol, eluting with acetone in activated silica gel column and evaporating to obtain purified product.

Description

technical field [0001] The invention relates to the field of biotechnology, specifically a kind of 1,2-diacyl-3-(6-deoxy-6-sulfonic acid group-α-D-glucopyranosyl)-sn-glyceride Preparation. Background technique [0002] AIDS (AIDs) is a systemic immunodeficiency disease caused by human immunodeficiency virus (HIV) infection, and is one of the most dangerous infectious diseases in the world. Since the first patient was discovered in 1981, the number of infected people worldwide has reached nearly 40 million, and the number of new infections is still increasing at a rate of 16,000 every day. About 90% of new infections appear in developing countries, and Asia accounts for 42% of new infections. AIDS patients have low systemic immune function and are prone to various complications and die. At present, there are only a dozen anti-AIDS drugs approved by the U.S. Food and Drug Administration (FDA), mainly reverse transcriptase inhibitors, which are prone to drug resistance. Altho...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07H17/04
Inventor 范晓韩丽君李宪璀娄清香
Owner INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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