Process for preparing high active composite cellulase special for woody fodder

A compound cellulase and high activity technology, which is applied in the field of compound cellulase preparation for high activity woody feed, can solve the problems of low enzyme activity, unbalanced enzyme system and high cost, and achieves high enzyme production activity and high enzyme system. Balanced, low-cost effects

Inactive Publication Date: 2006-05-10
SHENYANG INST OF APPL ECOLOGY CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In order to solve the shortcomings of low enzyme activity, unbalanced enzyme system, and high cost in the above-mentioned enzyme preparation products, the purpose of the present invention is to provide a low-cost, high-activity woody feed compound cellulase preparation method

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] (1) Incline cultivation

[0021] Insert Trichoderma konii F244 into a test tube equipped with PDA medium (20% potato, 2% glucose, 2% agar, and the remainder is water), and culture it statically at 28° C. for 6 days;

[0022] (2) Preparation of spore suspension

[0023] Wash the Trichoderma konii spores on the slant medium with sterile water to make a spore suspension;

[0024] (3) Shake flask expansion culture

[0025] Insert the slant spore suspension of a slant into a 500ml shake flask containing 100ml of PDY medium (20% of potato, 2% of glucose, 1% of yeast extract, and the remainder is water), expand the culture, at 28°C, 130r / min Cultivate under conditions for 3 days to obtain a shake flask spore suspension;

[0026] (4) Enzyme-producing solid fermentation:

[0027] Take 50g of the above-mentioned solid medium for autoclaving, inoculate 2ml of shake flask spore suspension, and then adopt dual-temperature culture method to ferment for 96 hours; keep the temperat...

Embodiment 2

[0031] The difference from Example 1 is:

[0032] Insert Trichoderma konshii into the test tube with PDA medium, and culture it statically at 32°C for 9 days; insert the slant spore suspension into the PDY medium, expand the culture, at 25°C, 80r / min Cultivate for 10 days to obtain a shake flask spore suspension; when producing enzyme solid fermentation, take 50 g of the above-mentioned solid medium and carry out high-pressure sterilization, inoculate 4 ml of shake flask spore suspension, and then adopt a dual-temperature culture method to ferment for 144 hours; the first 48 hours The high-activity composite solid enzyme preparation can be prepared by constant temperature of 35°C, 22°C for the last 96 hours, and control of ambient humidity at 60%. Wherein: the formula of solid medium is: rice straw powder: 25%, wheat bran: 15%, (NH 4 ) 2 SO 4 : 5%, moisture: 55%. Initial pH: Natural, about 6.4.

[0033] After the fermentation, the enzyme activity of the filter paper was m...

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PUM

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Abstract

Said invention relates to a method for preparing wood fodder especially a high activity wood fodder composite cellulase, which uses Trichoderma koningii as enzyme producing bacterial seeding by double temperature culturing method to make solid fermentation through inclined surface culturing, bottle swing extending culturing and novel solid culture medium to obtain said products.

Description

technical field [0001] The invention relates to a woody feed, especially a high-content crude fiber production feed, in particular to a preparation method of a special compound cellulase for a high-activity woody feed. Background technique [0002] Shrubs such as caragana and seabuckthorn are common greening tree species in the Ordos Plateau of Inner Mongolia Autonomous Region, which have the advantages of resistance to barrenness, low temperature and drought. At present, the planting area of ​​Caragana alone reaches hundreds of thousands of hectares. With the implementation of the local grazing prohibition policy, it is imperative to raise livestock in captivity, but the shortage of forage has become a limiting factor for the realization of captive livestock in the local area. Woody feed has always been a potential forage resource, such as caragana, which is known as "relief grass" and "life-saving grass", but its high content of crude fiber and pectin not only affects the...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N9/42C12N1/14A23K1/165C12R1/885A23K20/189
Inventor 李培军靖德兵张春桂许华夏寇振武丁建国李颖梅张海荣
Owner SHENYANG INST OF APPL ECOLOGY CHINESE ACAD OF SCI
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