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Method for screening aerobic denitrifying bacteria

A technology of aerobic denitrifying bacteria and strains, which is applied in the direction of biochemical equipment and methods, measurement/inspection of bacteria and microorganisms, and can solve problems such as increased processing costs, high engineering costs, and secondary pollution

Inactive Publication Date: 2006-10-11
HARBIN INST OF TECH
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Problems solved by technology

However, the traditional biological denitrification technology, because the demand for oxygen of nitrifying bacteria and denitrifying bacteria is completely opposite, it is difficult to unify the two processes of nitrification and denitrification in time and space, and has the following disadvantages: (1) High engineering cost, The operation control is complicated; (2) The acidity generated in the nitrification process needs to be neutralized by adding alkali, which not only increases the treatment cost, but also may cause secondary pollution; (3) The system has poor impact resistance and high concentration of NH 4 + , NO 2 - inhibits the growth of nitrifying bacteria

Method used

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  • Method for screening aerobic denitrifying bacteria
  • Method for screening aerobic denitrifying bacteria

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specific Embodiment approach 1

[0005] Specific embodiment 1: This embodiment is carried out according to the following steps: a. Enrichment of aerobic denitrifying bacteria: adopt the method of domesticating anaerobic denitrification sludge to enrich the biological flora, the interaction between microorganisms and the environment, microorganisms and microorganisms The effect is complex and subtle. Sudden changes in environmental conditions (from anaerobic / anoxic to aerobic) will not only fail to enrich the effect, but will also destroy the original ecological balance and damage the original flora, so adopt Means of acclimatizing anaerobic denitrification sludge (acclimatizing devices such as figure 1 As shown), gradually change the environmental conditions, that is, gradually increase the aeration rate, aeration time, and the concentration of nitrate and nitrite in the influent, to achieve the environmental conditions for the strains to be screened, and give the biological flora a process of adaptation, and ...

specific Embodiment approach 2

[0006] Specific implementation mode two: this implementation mode is realized in this way:

[0007] a. Enrichment of aerobic denitrifying bacteria: (1) Take activated sludge from SBR method for treating high-concentration ammonia-nitrogen wastewater or traditional anaerobic denitrification sludge process; (2) Initial domestication, only use FM distribution water as influent , every 2 to 3 days, increase the content of the DM water distribution culture solution in the distribution water until all the distribution water is DM culture solution, the COD of the influent water is controlled at 5000 mg / L, the total nitrogen (TN) is at 500 mg / L, and during the acclimation process In the process, after maintaining the original dissolved oxygen (DO) concentration of the sludge for 2 to 3 days, while changing the water distribution, DO is also increased by 0.5 to 1 mg / L. The second is to gradually reach the suitable conditions for aerobic denitrifying bacteria, so that the bacteria can b...

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Abstract

A method for screening aerobic denitrifying bacteria relates to a method for efficiently screening aerobic denitrifying bacteria. The present invention is carried out according to the following steps: a. adopting the method of domesticating anaerobic denitrification sludge to enrich the biological flora; b. adopting the plate dilution method and the plate streaking method to the biological flora in the activated sludge obtained after domestication Separation and purification; c. Preliminary screening of pure bacteria obtained after separation and purification to obtain strains with a TN removal rate of over 50%; d. Rescreening of primary screening strains to obtain aerobic denitrifying bacteria. The realization of the aerobic denitrification technology of the present invention can not only overcome the disadvantages of the traditional biological denitrification technology, but also has the following advantages: (1) can realize denitrification and COD removal in the same reactor; (2) under the regulation of feedback Shorten the residence time; (3) reduce the aeration; (4) the alkalinity produced by the denitrification process can neutralize the acidity produced by the nitrification process, and reduce the alkalinity consumption; (5) help to realize short-range synchronous nitrification and denitrification .

Description

technical field [0001] The invention relates to a method for efficiently screening aerobic denitrifying bacteria. Background technique [0002] It is generally believed that denitrifying bacteria that perform nitrate respiration are facultative anaerobic microorganisms, because only under anaerobic conditions can they induce nitrate reductase A and nitrite reductase required for denitrification. However, in recent years, people have continuously found the loss of total nitrogen under aerobic conditions, so the research on aerobic denitrification has gradually attracted the attention of researchers. At present, the cause of aerobic denitrification phenomenon is still inconclusive. Most researchers at home and abroad study aerobic denitrification technology from the production of local anaerobic or micro-environment anaerobic. The operation control is complicated and the system is unstable. Nitrogen in water can cause eutrophication, so the removal of nitrogen has attracted w...

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/02C12N1/20
Inventor 马放周丹丹任南琪杨基先
Owner HARBIN INST OF TECH