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Biology method for synthesizing beta alanine

A technology of biosynthesis and alanine, applied in the field of biosynthesis of β-alanine, can solve the problems of high cost and environmental pollution, and achieve the effect of low cost, high product purity and simple steps

Inactive Publication Date: 2006-11-22
ZHEJIANG UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to provide a kind of biosynthesis method of β-alanine, to overcome the deficiency that the existing chemical synthesis method of β-alanine is easy to pollute the environment and has high cost

Method used

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  • Biology method for synthesizing beta alanine
  • Biology method for synthesizing beta alanine
  • Biology method for synthesizing beta alanine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Example 1: Biosynthesis of β-alanine

[0041] Seed medium: corn steep liquor 1.1g, MgSO 4 .7H 2 O 0.02g, KH 2 PO 4 0.1g, 0.145g sodium chloride, 0.5g beef extract, 100mL water, sterilized at 121℃ for 20min, 0.8g acrylic acid, adjust pH to 7.0 with sodium hydroxide;

[0042] Fermentation medium: corn steep liquor 1.1g, MgSO 4 .7H 2 O 0.02g, KH 2 PO 4 0.1g, sodium chloride 0.145g, water 100mL sterilized at 121℃ for 20min, acrylic acid 0.8g, sodium hydroxide to adjust pH to 7.0;

[0043] Conversion solution: 15g of acrylic acid, MgSO 4 .7H 2 O 0.11g, adjust the pH to 7.5 with ammonia water, and dilute to 500mL with 0.2M phosphate buffer.

[0044] The slant strain of Sarcina luteus was inoculated into the seed medium, cultured on a shaker at 200 r / min at 30°C for 24 hours. 20% of the inoculum was transferred to the fermentation medium, 30° C., 200 r / min shaker culture for 18 hours to obtain the fermentation broth. The fermented liquid was added into the conver...

Embodiment 2

[0045] Example 2: Biosynthesis of β-alanine

[0046] Seed medium: corn steep liquor 0.5g, MgSO 4 .7H 2 O 0.02g, KH 2 PO 4 0.05g, sodium chloride 0.10g, beef extract 0.8g, water 100mL sterilized at 121℃ for 20min, acrylic acid 0.08g, hydrochloric acid to adjust the pH to 6.5;

[0047] Fermentation medium: corn steep liquor 0.5g, MgSO 4 .7H 2 O 0.02g, KH 2 PO 4 0.05g, sodium chloride 0.10g, water 100mL sterilized at 121℃ for 20min, acrylic acid 0.08g, hydrochloric acid to adjust the pH to 6.5;

[0048] Conversion solution: Acrylic acid 10g, MgSO 4 .7H 2 O 0.10g, adjust the pH to 4.8 with ammonia water, and dilute to 500mL with 0.2M acetate buffer.

[0049] The slant strain of Sarcina luteus was inoculated into the seed medium, cultured on a shaker at 25°C for 20 hours at 100 r / min. 15% of the inoculum was transferred to the fermentation medium, and cultured on a shaker at 100 r / min at 25° C. for 16 hours. The fermented liquid is added to the conversion liquid in a ...

Embodiment 3

[0050] Example 3: Biosynthesis of β-alanine

[0051] Seed medium: corn steep liquor 1.8g, MgSO 4 .7H 2 O 0.02g, KH 2 PO 4 0.05g, 0.45g sodium chloride, 6.5g beef extract, 100mL water, sterilized at 121°C for 20min, 7.5g acrylic acid, pH 5.5 with hydrochloric acid;

[0052] Fermentation medium: corn steep liquor 1.8g, MgSO 4 .7H 2 O 0.02g, KH 2 PO 4 0.05g, 0.45g of sodium chloride, 100mL of water, sterilized at 121°C for 20min, 7.5g of acrylic acid, adjusted to pH 5.5 with hydrochloric acid;

[0053] Conversion solution: 200g of acrylic acid, MgSO 4 .7H 2 O 0.12g, adjust the pH to 6.0 with ammonia water, and dilute to 500mL with 0.1M citric acid buffer.

[0054] Escherichia coli slant strains were inserted into the seed medium, cultured on a shaking table at 55° C. for 18 hours at 300 r / min. 15% of the inoculum was transferred to the fermentation medium, and cultured on a shaker at 55° C. at 300 r / min for 16 hours. The fermented liquid is added to the conversion l...

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Abstract

The invention provides a biosynthetic method of β-alanine, especially a method for synthesizing β-alanine under the action of microbial enzymes. The corresponding aminase is cultivated in the seed culture medium containing acrylic acid and the fermentation medium containing acrylic acid, and the aminase is added into the transformation liquid, and the transformation liquid contains 1% to 50% (g / ml) Ammonia solution of acrylic acid, synthesize β-alanine under the action of enzyme, and get β-alanine product through deamination and purification. The method uses microbial enzymes as catalysts, and has high reaction efficiency and no pollution; the reaction raw materials are easy to obtain, the steps are simple, and the cost is low; and the product has high purity.

Description

(1) Technical field [0001] The invention relates to a biosynthetic method of beta-alanine, in particular to a method for synthesizing beta-alanine under the action of microbial enzymes. (2) Background technology [0002] β-alanine, also known as β-alanine. In 1972, it was discovered by Ross and Monroe in the degradation products of uracil. It is a non-protein amino acid and the only β-type amino acid in nature. The main physiological activity of β-alanine is to synthesize pantothenic acid and coenzyme A. Modern medical research has found that in the nervous system of mammals, it can be used as a neurotransmitter in the brain; it can activate ion channels; it can treat liver damage caused by tissue hypoxia. [0003] In the field of fine chemical industry, β-alanine is used for the synthesis of polymerized β-alanine, electroplating buffer, dyes and other production. Polymerized β-alanine is used as a flocculant in the water treatment industry; in the pharmaceutical industr...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P13/06
Inventor 裘娟萍沈寅初姚燕来韩建娜黄海婵汪琨
Owner ZHEJIANG UNIV OF TECH
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