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Simple method for screening sulfadiazing (SD) degradating bacterial for treating SD-contxined sewage

A technology of sulfadiazine and aerobic biology, applied in the direction of aerobic process treatment, sustainable biological treatment, biological water/sewage treatment, etc., can solve the problem of failing to meet the design standards and current needs, and the removal rate of COD is less than 50%. , SD wastewater treatment efficiency is low, etc., to improve the total COD removal rate, reduce the average treatment cost, and achieve the effect of clean production

Inactive Publication Date: 2006-12-20
SHENYANG INST OF APPL ECOLOGY CHINESE ACAD OF SCI
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Problems solved by technology

Due to the strong antibacterial effect of the main components of wastewater, the degradation of activated sludge activity year by year, and the unstable operation of original equipment, the current treatment efficiency of SD wastewater is very low. In most cases, the COD removal rate is less than 50%. Fall short of design standards and current needs

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Embodiment Construction

[0019] In the present invention, the SD degrading bacteria selective medium and the complete bacterial medium are alternately / combined to specifically screen high-efficiency SD degrading bacterial strains, that is, the selective medium is used for preliminary screening, and then the complete medium is used for rejuvenation, and finally the Selective media validation. Preliminary screening with selective medium greatly improved the screening efficiency, rejuvenation with complete medium greatly shortened the screening cycle, and verification with selective medium improved the accuracy of screening.

[0020] SD degrading bacteria selective medium uses sulfadiazine as the only carbon and nitrogen source; common bacterial complete medium is yeast extract peptone medium. The main steps of this simple screening method include:

[0021] 1) After diluting the inoculum source, spread it on the selective medium;

[0022] 2) transfer the single bacterium colony grown on the selective m...

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Abstract

A simple method for screening the sulfadiazine (SD) degradating bacteria used to effectively treat the SD contained sewage by aerobic deep-well method includes such steps as using the selective culture medium for primarary screening, using the complete culture medium for rejuvenation, and using the selective culture medium for verification.

Description

technical field [0001] The invention belongs to the technical field of wastewater biological treatment, in particular to a simple screening method for highly efficient aerobic biological treatment strains of sulfadiazine wastewater. Background technique [0002] High-concentration wastewater from pharmaceutical companies—sulfadiazine wastewater (SD wastewater) is wastewater generated during the chemical synthesis of sulfadiazine. Taking a certain enterprise as an example, its daily discharge of sulfadiazine wastewater is about 180 tons, and its COD value is 10,000-20,000 mg / L. Synthesis of sulfadiazine by chemical method is to use propynyl alcohol, diethylamine, active manganese dioxide and sulfadiazine as raw materials, through ammoxidation, cyclization, acid precipitation and other steps to form sulfadiazine, the total yield is 83.5%. The main component of SD wastewater is sulfadiazine (chemical name: N-2-pyrimidinyl-4-aminobenzenesulfonamide, English name: Sulfadiazine, ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C02F3/02C02F3/34
CPCY02W10/10
Inventor 徐慧史荣久张颖朗咸明王卓陈冠雄
Owner SHENYANG INST OF APPL ECOLOGY CHINESE ACAD OF SCI
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