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Clone of gene MAC30 related to pig backfat thickness and its use in mark-assisted selection

A technology for pig backfat thickness and genes, applied in the field of livestock genetic engineering, can solve problems such as the lack of comprehensive research on the function of the MAC30 gene.

Inactive Publication Date: 2007-02-14
HUAZHONG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But so far, there is still no comprehensive study on the function of MAC30 gene.

Method used

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  • Clone of gene MAC30 related to pig backfat thickness and its use in mark-assisted selection
  • Clone of gene MAC30 related to pig backfat thickness and its use in mark-assisted selection
  • Clone of gene MAC30 related to pig backfat thickness and its use in mark-assisted selection

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0062] Example 1: Association analysis of pig marker traits

[0063] The correlation analysis between A848G polymorphism of exon 3 of MAC30 gene and some production traits was carried out in a Tongcheng pig herd. The analyzed traits were some carcass traits and some meat quality traits.

[0064] The results of genotype detection showed that the GG genotype accounted for the vast majority in 40 individuals, and 15 individuals had the AG genotype. The results of the significant differences in traits among different genotypes (least square mean and standard error analysis) are summarized in Table 2. The analysis results show that the average backfat thickness of the GG genotype and the backfat thickness at ribs 6-7 were average. Very significantly lower than AG genotype pigs (P<0.01), other traits have no significant difference between different genotypes.

[0065]

[0066] ** Indicates that the difference is extremely significant at the 0.01 level.

Embodiment 2

[0067] Example 2: Distribution of porcine MAC30 gene 848 polymorphism in various pig breeds

[0068] The polymorphism at the 848th position of porcine MAC30 gene was detected in 6 pig breeds. The frequency of gene G is dominant, but in foreign commercial pig breeds Landrace and Duroc, no A allele was detected.

[0069]

[0070] The difference in distribution of polymorphic gene frequency of porcine MAC30 gene 848 in different breeds was tested, and the result of significant difference showed that the gene frequency of this locus had a large degree of difference among the six tested pig breeds.

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Abstract

The present invention discloses pig back fat thickness relevant gene MAC30 mononucleotide polymorphism site detecting method and its application as molecular mark for auxiliary selection in genetic breeding of pig. Partial genome gene is cloned from pig back fat thickness relevant gene MAC30, and the gene has DNA sequence as shown in SEQ ID No. 1 in the sequence list, with whole length of 970 bp and including partial exon 2 and partial exon 3 as shown in Fig. 2. In the sequence, there is one base mutant (848G-848A) in the site of 848 bp to result in coded amino acid change from valine to isoleucine (GTC-ATC). The detection on Chinese local pig varieties and European blood line pig varieties proves the effect of the present invention in auxiliary pig genetic selection. The present invention also provides method of preparing the said gene and applying it in detection.

Description

technical field [0001] The invention belongs to the technical field of livestock genetic engineering, and in particular relates to the cloning of pig backfat thickness-related gene MAC30 and its application in marker-assisted selection. Background technique [0002] China is a big pig-raising country, and it is also the country with the richest pig breed resources. Wild boars have been domesticated into domestic pigs very early. Through long-term selection, many local breeds with their own characteristics have been formed, only 48 of which are recorded in "Chinese Pig Breeds Chronicle" (Zhang Zhongge, Shanghai Science and Technology Press, 1986). Compared with foreign commercial pigs, Chinese local breeds have many outstanding advantages: good meat quality, high litter size, good stress resistance and strong adaptability, etc., but they have two common shortcomings, that is, lean meat percentage Low and slow weight gain. Therefore, increasing carcass lean meat percentage a...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/12C12N15/10C07H21/00C12Q1/68
CPCC12Q2600/156C12Q2600/124C12Q1/6888
Inventor 李奎杨金娥余梅刘榜樊斌朱猛进熊统安
Owner HUAZHONG AGRI UNIV