Promoters and usage thereof
A technology of promoters and DNA molecules, applied in the field of promoters and their applications
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Embodiment 1
[0059] Example 1: acuF promoter
[0060] 1. Materials
[0061] (1) Host: Monascus pilosus BCRC 38072, Monascus pilosus, BCRC 31527, Neurospora crassa BCRC 32685 (Food Industry Development Research Institute).
[0062] (2) Competent cells: ECOS TM E.coli competent cells DH5α (Yisheng Biotechnology Company).
[0063] (3) Plasmid: pHygEGFP (BD Biosciences), pGEM _ -T EasyVector(PROMEGA).
[0064] (4) Medium:
[0065] a. Culture medium for Escherichia coli: LB broth (USB), agar (USB).
[0066] b. The medium of Monascus and pink bread mold (Neurospora crassa): PDA (DIFICO), PDB (DIFICO), Vogel's medium (see * ), and topagar.
[0067] (5) Antibiotics: penicillin (Ampicillin), hygromycin (Hygromycin B) (SIGMA).
[0068] * Vogel's Medium
[0069] 1. Trace element solution: 5g citric acid·H 2 O, 5g ZnSO 4 ·7H 2 O, 1gFe(NH 4 ) 2 (SO 4 ) 2 ·6H 2 Q. 250mg CuSO 4 ·5H 2 O, 50mgMnSO 4 ·H 2 O, 50 mg H 3 BO 3 , 50mg Na 2 MoO 4 2H 2 O was dissolved in 95ml of sterile...
Embodiment 2
[0143] Example 2: Hsp promoter
[0144] 1. Materials:
[0145](1) Host: Monascus BCRC 38072, Monascus pilosus BCRC 31527, and Neurospora crassa BCRC 32685 (Food Industry Development Research Institute).
[0146] (2) Competent cells: ECOS TM E.coli competent cells DH5α (Yisheng Biotechnology Company).
[0147] (3) Plasmid: pHygEGFP (BD Biosciences), pGEM _ -T EasyVector(PROMEGA).
[0148] (4) Medium:
[0149] a. Culture medium for Escherichia coli: LB broth (USB), agar (USB).
[0150] b. The medium of Monascus and pink bread mold (Neurospora crassa): PDA (DIFICO), PDB (DIFICO), vogel's medium (see * ), and topagar.
[0151] (5) Antibiotics: penicillin (Ampicillin), hygromycin (Hygromycin B) (SIGMA).
[0152] * Vogel's Medium
[0153] 1. Trace element solution: 5g citric acid·H 2 O, 5g ZnSO 4 ·7H 2 O, 1g Fe(NH 4 ) 2 (SO 4 ) 2 ·6H 2 O, 250mg CuSO 4 ·5H 2 O, 50mgMnSO 4 ·H 2 O, 50 mg H 3 BO 3 , 50mg Na 2 MoO 4 2H 2 O was dissolved in 95ml of sterile deio...
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