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Serine protease variants having amino acid deletions and substitutions

A serine protease and amino acid technology, applied in the field of serine protease variants and compositions comprising the variants, capable of solving problems such as the reduction of the degree of immune response

Inactive Publication Date: 2001-07-11
THE PROCTER & GAMBLE COMPANY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the process, the present inventors have discovered subtilisins which still retain their activity as efficient, cleaning proteases, but which elicit a reduced degree of immune response

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 7-10

[0119] pH=7 for all formulations.

[0120] The variants listed in Tables 2-10 and other variants such as the preferred variants mentioned herein can be substituted for Δ79 in Examples 7-10 with substantially similar cleaning effects.

[0121] The liquid fabric cleaning compositions of the present invention are illustrated by the following examples.

Embodiment 11-13

[0124] The variants listed in Tables 2-10 and other variants such as the preferred variants mentioned herein can be substituted for Δ70, 75-82 in Examples 11-13 with substantially similar cleaning effects.

[0125] personal care composition

[0126] The variants of the present invention are especially useful as personal care compositions such as leave-on and rinse-off conditioners, shampoos, leave-on and rinse-off acne cleansing compositions, cleansers and body lotions, shower gels, soaps, Foaming and non-foaming face washes, cosmetics, hand, face and body washes and moisturizers, leave-in facial moisturizers, cosmetic scrubs, oral care compositions and contact lens care solutions. These personal care compositions contain one or more variants of the invention together with a personal care carrier.

[0127] In particular, details on variants suitable for use in these compositions are found in the following references: U.S. Patent No. 5,641,479, Lin...

Embodiment 14-17

[0135] Example

[0136] The variants listed in Tables 2-10 and other variants such as the preferred variants mentioned herein can be substituted for Δ75-82 in Examples 14-17 with substantially similar cleaning effects.

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PUM

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Abstract

The present invention relates to variants of serine proteases having decreased immunogenicity relative to their corresponding wild-type proteases. More particularly, the present invention relates to variants having a modified amino acid sequence of a wild-type amino acid sequence, wherein the modified amino acid sequence comprises a deletion and, optionally, a substitution of one or more specifically identified positions corresponding to subtilisin BPN'. The invention further relates to mutant genes encoding such variants and cleaning and personal care compositions comprising such variants.

Description

field of invention [0001] The present invention relates to serine protease variants, which have reduced immunogenicity compared to the corresponding wild-type serine protease, and compositions comprising the variants. Background of the invention [0002] Among naturally occurring proteins, enzyme proteins are the most abundant. There is a class of enzymes that includes proteases that catalyze the hydrolysis of other proteins. Its ability to hydrolyze proteins has been exploited by incorporating naturally occurring proteases and proteases produced through protein engineering into cleaning compositions, especially those used to launder clothes. [0003] In the field of clean technology, the most widely used proteases in this class are serine proteases. Most serine proteases are produced by bacteria, and some are produced by other organisms, such as fungi. See Siezen, Roland J. et al., "Homology Modeling and Protein Engineering Strategy of Subtilases,...

Claims

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Application Information

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IPC IPC(8): C12N15/09A61K8/00A61K8/02A61K8/66A61K8/96A61Q5/00A61Q5/02A61Q11/00A61Q19/00A61Q19/10C12N9/54C12N15/57
CPCA61Q19/00A61K8/66A61Q11/00A61K8/0208A61Q5/00A61Q19/10A61K2800/86C12N9/54C12N9/50
Inventor D·N·鲁冰E·E·斯科尔斯基P·E·科雷
Owner THE PROCTER & GAMBLE COMPANY
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