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Cowpea assortment analyzing and determining method based on salting-in-protein graphical spectrum technology

The technology of a salt-soluble protein and an identification method is applied in the field of analysis and identification of cowpea varieties based on the salt-soluble protein map technology, and the effect of low identification cost is achieved.

Inactive Publication Date: 2007-03-28
JIANGHAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The technical problem to be solved by the present invention is to provide a new analysis and identification method for cowpea varieties based on salt-soluble protein map technology to make up for the deficiencies of the existing methods

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  • Cowpea assortment analyzing and determining method based on salting-in-protein graphical spectrum technology
  • Cowpea assortment analyzing and determining method based on salting-in-protein graphical spectrum technology
  • Cowpea assortment analyzing and determining method based on salting-in-protein graphical spectrum technology

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Embodiment Construction

[0024] The present invention will be further described below in conjunction with accompanying drawing:

[0025] The present invention comprises the following steps:

[0026] (1) extract the salt-soluble protein in the existing cowpea variety, carry out polyacrylamide gel electrophoresis analysis respectively, obtain the salt-soluble protein standard sample collection of illustrations of each cowpea variety, as shown in Figure 1;

[0027] (2) Establish a database according to the subunit distribution information of the salt-soluble protein bands of each cowpea variety, and obtain a phylogenetic cluster tree through cluster analysis. According to the characteristics of the phylogenetic cluster tree, each cowpea variety can be divided into a variety group and a variety subgroup, as shown in the figure 2 shown;

[0028] (3) Carry out gel electrophoresis analysis to the cowpea variety that needs to be identified according to the above steps, obtain the salt-soluble protein spectru...

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Abstract

This invention relates to plant specimen identification method and in detail to cowpea specimen analysis and identification method based on salt solution protein atlas technique, which comprises the following steps: extracting cowpea specimen salt dissolved protein for separately polypropylene amide gel electrophoretic analysis to get the cowpea specimen protein atlas; going for electrophoretic analysis gel of the needed cowpea specimen by the above steps to get the cowpea specimen protein atlas; comparing the protein atlas with the standard atlas to get the analysis and identification results.

Description

technical field [0001] The invention relates to a plant variety identification method, in particular to a cowpea variety analysis and identification method based on salt-soluble protein map technology. Background technique [0002] Cowpea [Vigna unguiculata (L.) Walp.] is an annual herbaceous plant, which originated in tropical Africa and is widely distributed in tropical and subtropical regions. There are three cultivated subspecies of cowpea: common cowpea (Vigna unguiculata ssp. unguiculata), long pod cowpea (Vigna unguiculatassp. sesquipedalis), and short pod cowpea (Vigna unguiculata ssp. cylindrica). Correct division of variety groups can clearly reflect the distance of kinship between cowpea varieties, which is of great significance for parent selection, resource conservation and utilization in plant genetics and breeding. [0003] In the past, the classification and identification of cowpea varieties were mainly based on the division method of qualitative traits, an...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N27/447G01N30/00G01N30/86G01N33/68G06F17/00
Inventor 陈禅友胡志辉赵新春
Owner JIANGHAN UNIVERSITY