Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for visual detection of inorganic phosphorus in urine and kit thereof

A visual detection and inorganic phosphorus technology, which is applied in biological testing, material inspection products, color/spectral characteristic measurement, etc., can solve the problems of lack of convenience and popularity, and the inability to realize the visual use of kits, etc., and achieve wide popularity and social value, the effect is obvious, and the effect of portability

Inactive Publication Date: 2007-05-23
SHANXI UNIV
View PDF3 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It should be pointed out that the above clinical tests, including the method we invented, are based on the matching of the kit with the UV-Vis spectrometer, and the visual use of the kit cannot be realized.
Due to the lack of large-scale equipment, small hospitals and clinics cannot use the determination of inorganic phosphorus in patients' urine as a routine clinical test
Therefore, the above clinical testing method lacks convenience and universality.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for visual detection of inorganic phosphorus in urine and kit thereof
  • Method for visual detection of inorganic phosphorus in urine and kit thereof
  • Method for visual detection of inorganic phosphorus in urine and kit thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Weigh HEPES (C 8 h 18 N 2 o 4 S, FW=238.30) 0.238g, dissolved in 95ml of distilled water, then adjusted the pH to 7.0 with 0.1M NaOH, and finally set the volume to 100ml to obtain a 10mM HEPES buffer solution, marked as R 1 ; Weigh catechol violet (C 19 h 14 o 7 S, FW=386.30) 0.0386g, dissolved in distilled water and fixed to 25ml to obtain 2mM catechol violet solution, marked as R 2 ; Weigh YbCl 3 (FW=279.5), dissolved in distilled water and fixed to 25ml to obtain 2mM Yb 3+ solution, labeled R 3 . Put 2ml of R 1 Add the solution to a clean colorimetric tube, and use a micro-sampler to draw R 2 50 μl and R 3 100μl was added to the colorimetric tube, and the solution was blue at this time. In the above-mentioned colorimetric tube, take 4 μl of adult urine sample and add it to the colorimetric tube with a micro-sampler. After shaking, the color is blue-green. Compared with the standard color scale, it is found that the concentration of inorganic phosphorus i...

Embodiment 2

[0027] Prepare HEPES (10mM) buffer solution with pH 6.5, and the others are the same as in Example 1, so that the content of inorganic phosphorus in the urine ranges from 12-20mM. The results are shown in Figure 3.

Embodiment 3

[0029] Take the urine of a one-year-old healthy child, and the rest are the same as in Example 1, but the obtained color is pale yellow. Compared with the standard color scale, it is found that the content range of inorganic phosphorus in the child's urine sample is 42-50mM. The results are shown in Figure 4. From the above examples, we can see that the content of inorganic phosphorus in children's urine is relatively high. This is because compared with adults, children have higher alkaline phosphatase activity and similar metabolic rate because they are in the period of vigorous osteogenesis. Wait for the reason.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

This invention is a method and reagent box, which is used to examine the inorganic phosphorus in urine by visual observation. This method uses Yb ion and uses pyrocatechol violet as developer. First, put ion into HEPES buffer solution, whose pH is 6.5-7.5, then compare its color with the reference color and determine the consistency of inorganic phosphorus. This method can be used in many hospitals, clinics and can also used at home.

Description

Technical field: [0001] The invention relates to a method for detecting inorganic phosphorus, in particular to a method for clinically visually detecting inorganic phosphorus in urine and a kit thereof. Background technique: [0002] Inorganic phosphorus is involved in various important biomineralization processes, such as bone formation and pathological processes such as the origin of kidney stones. Therefore, the detection of inorganic phosphorus in biological fluids is important. We know that urine has a very important diagnostic value in clinical events, so it is necessary to detect inorganic phosphorus in human urine. Too much or too little inorganic phosphorus in urine can cause stone disease, prostate disease, hypoparathyroidism or hyperparathyroidism, advanced chronic nephritis, multiple myeloma, rickets or rickets, etc., if not cured in time, it may even cause greater of other diseases. Therefore, it is critical to maintain a certain concentration of inorganic ph...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/84G01N21/78G01N21/29
Inventor 阴彩霞杨频霍方俊
Owner SHANXI UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com