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Batyl active peptide, preparation and therepic use thereof

A technology of active polypeptide and shark liver, applied in the field of prevention and treatment of diabetes, can solve the problems of purity less than 95%, single, etc.

Inactive Publication Date: 2007-06-13
CHINA PHARM UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the polypeptide used for liver damage protection is not a single pure product, and its purity has not yet reached more than 95%.

Method used

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  • Batyl active peptide, preparation and therepic use thereof
  • Batyl active peptide, preparation and therepic use thereof
  • Batyl active peptide, preparation and therepic use thereof

Examples

Experimental program
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Embodiment 2

[0047] Preparation of centrifuged supernatant: Take 5kg of the quarantined baby shark liver, mince it, add 10000ml of distilled water to homogenate, heat the homogenate to 95°C, keep it for 5 minutes, and then heat it at 8000rpm -1 Refrigerate and centrifuge for 20 minutes under the conditions, discard the precipitate, take the supernatant, and use the Folin-phenol method to measure its protein content to be 12.62 mg / ml.

[0048] The above supernatant was ultrafiltered with hollow fiber membranes with a molecular weight cut-off of 60KD and 30KD, the filtrate was collected, and its protein content was measured to be 6.01mg / ml by the same method.

[0049] Use DEAE-Sephadex A25 for column chromatography, the column bed volume is 1500ml, load 8.0g sample, wash the unbound foreign protein with 0.02mol / L PBS buffer solution with pH8.0, the flow rate is 60ml / h, wash to A 280 <0.05, then eluted with 0.02 mol / L pH7.0 PBS buffer containing 0.2 mol / L NaCl at a flow rate of 60 ml / h, colle...

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Abstract

Batyl bioactive peptide, its production and use are disclosed. Its relative molecular weight is 8300alton, isoelectric point is 5.2, and ultraviolet-characterized absorbing peak is 275nm. It can be used to prevent and treat diabetes mellitus.

Description

technical field [0001] The invention belongs to the field of biomedicine, and specifically relates to a new polypeptide isolated from shark liver by bioengineering technology, its preparation method and its use for preventing and treating diabetes. Background technique [0002] Sharks are one of the most active animals in the ocean, with their liver accounting for 70% of their internal organs. Shark liver active peptide is an active polypeptide isolated and purified from shark liver, that is, a shark hepatic stimulatory substance (sHSS). Like other sources of liver stimulatory substances, it can specifically promote DNA synthesis in liver cells. Prevent liver damage and restore liver function. Our research group has carried out long-term and extensive research on shark liver active peptides, and found that it has protective effects on immune liver damage and also has immunoregulatory effects (see "Chinese Journal of New Drugs" 2001, 10(1): 29-31) ; It can alleviate the inh...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K14/435C07K1/16A61K38/17A61P3/10
Inventor 吴梧桐黄凤杰
Owner CHINA PHARM UNIV