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Implantation method of sex-linked balance lethal and sex control gene for Bombyx mori

A technology that balances lethality and gender. It is applied in genetic engineering, plant genetic improvement, and botanical equipment and methods. It can solve the problems of no direct use value and poor economic traits, and achieve the effect of fast improvement and good effect.

Inactive Publication Date: 2002-04-17
ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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Problems solved by technology

Therefore, the economic traits of the introduced species are very poor, far inferior to the current silkworm species in my country, and have no direct use value in our country. The introduced species must be improved by transgenic to maintain the excellent economic properties of the current species without losing the sex of the balanced lethal line. control gene

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  • Implantation method of sex-linked balance lethal and sex control gene for Bombyx mori
  • Implantation method of sex-linked balance lethal and sex control gene for Bombyx mori
  • Implantation method of sex-linked balance lethal and sex control gene for Bombyx mori

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Embodiment Construction

[0014] A method for sex-controlling gene transfer in a sex-linked balanced lethal line of Bombyx mori

[0015] 1. Balance the sex control gene structure of the lethal line

[0016] figure 1 The structure and schematic diagram of the lethal sex control gene in the balance of sex continuum. From figure 1 It can be seen that the two sex chromatids Z of the male of this system each carry a recessive homozygous embryonic lethal gene with a mutation, namely l 1 and l 2 . The exchange rate of the two genes is 0.8%; there is a recessive homozygous second albumen gene w on the 10th autosome 2 / w 2’ Thus the male gene structure is (according to the standard genotype should be written as ,w 2 / w 2 , for the convenience of writing, the normal gene is deleted without causing confusion. ) Simultaneous translocation of the sex-linked lethal gene l on the W sex chromosome of the female 1 and the second albumen w 2 allelic normal gene fragment and , the Z chromosome also ca...

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Abstract

By means of hybridization of balance lethal line and available variety, the sex control gene of balance lethal line is dispersed in three subsystems. The three subsystems are made to backcross with available variety continuously to obtain the blood relation of available variety; and the sex control genes are combined to obtain one new variety of balance lethal line, through three times of mating in different modes. The sex-linked balance lethal line of Bombyx mori can reproduce secondary generation through self-copulation and ensure male incubation only when crossbreeding with other variety. The present invention can breed new male Bombyx mori variety with excellent economic characteristic and male Bombyx mori rate over 98 % in fast and high improvement effect.

Description

technical field [0001] The invention relates to a method for introducing a sex control gene of silkworm sex-linked balance lethal line. Background technique [0002] Compared with female silkworms, male silkworms have the obvious advantages of eating less mulberry, lower cost of raising silkworms, high silk yield and excellent silk quality. The special breeding of male silkworms can increase the economic benefit by more than 10% compared with the current mixed breeding of males and females. Therefore, China, Japan, Russia and other countries have studied silkworm sex control technology from different angles, and have not obtained a satisfactory male silkworm variety for half a century. Therefore, the technology of exclusively breeding male silkworms has not been applied in production practice. After decades of hard work, the Institute of Developmental Biology of the Russian Academy of Sciences has constructed a sex-linked balance lethal line of silkworms. The male silkworms...

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Application Information

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IPC IPC(8): A01K67/04C12N15/12
Inventor 何克荣夏建国祝新荣黄健辉
Owner ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES