Process for preparing chlorogenic acid

A technology for chlorogenic acid and raw materials, applied in the field of preparing chlorogenic acid, can solve problems such as no public reports, and achieve the effects of simple operation method, reduced production cost and low cost

Inactive Publication Date: 2003-08-13
SHANDONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Experiments have shown that burdock leaves contain a relatively high content of chlorogenic acid. If the burdock leaf waste can be fully utilized, the production cost of chlorogenic acid will be greatly reduced, but so far there has been no public report on this

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Fresh burdock leaves are naturally air-dried indoors, and 100g of dried burdock leaves are extracted twice at 78°C with 2000ml ethanol (pH2), 2 hours for the first time, 1 hour for the second time, suction filtration after each extraction, combined filtrates, and reduced pressure of the filtrates Concentrate and recover ethanol to obtain the crude extract of burdock leaves. Analysis by RP-HPLC, UV, LC-ESI-MS, etc. and standard comparison showed that the crude extract of burdock leaves contained chlorogenic acid, and chlorogenic acid was a main component of the crude extract of burdock leaves.

Embodiment 2

[0019] Dried burdock leaves, extract 150g of dried burdock leaves with 3000ml ethanol (pH3) at 77°C for 3 times and reflux for 1 hour for the first time, 1 hour for the second time, and 1 hour for the third time. Suction filter after each extraction, combine the filtrates, concentrate the filtrate under reduced pressure, recover ethanol, and obtain the crude extract of burdock leaves. Analysis by RP-HPLC, UV, LC-ESI-MS, etc. and standard comparison showed that the crude extract of burdock leaves contained chlorogenic acid, and chlorogenic acid was a main component of the crude extract of burdock leaves. Pass the crude extract of burdock leaves through a polyamide column, elute with water and methanol, and concentrate and refine the eluate to obtain pure chlorogenic acid.

Embodiment 3

[0021] Dried burdock leaves, extract 150g of dried burdock leaves with 2500ml ethanol (pH 2.5) in 4 times at 79°C and reflux for 1h for the first time, 1h for the second time, 0.5h for the third time, and 0.5h for the fourth time. Suction filter after each extraction, combine the filtrates, concentrate the filtrate under reduced pressure, recover ethanol, and obtain the crude extract of burdock leaves. Analysis by RP-HPLC, UV, LC-ESI-MS, etc. and standard comparison showed that the crude extract of burdock leaves contained chlorogenic acid, and chlorogenic acid was a main component of the crude extract of burdock leaves. Pass the crude extract of burdock leaves through a polyamide column, elute with water and methanol, and concentrate and refine the eluate to obtain pure chlorogenic acid.

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PUM

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Abstract

A process for preparing the chlorogenic acid used as medicinal component includes reflux extraction of dried arctium leaves in organic solvent at pH=2-3 several times for 3 hr, filtering per time, collecting filtrate, vacuum concentrating, recovering alcohol to obtain raw extract, chromatography by polyamide column, eluting with water and methanol, concentrating and refining. Its advantage is low cost.

Description

(1) Technical field [0001] The invention relates to a method for extracting medicinal components of plants, in particular to a method for preparing chlorogenic acid with low-cost burdock leaves as raw materials. (2) Background technology [0002] Chlorogenic acid (chlorogenic acid) belongs to caffeoylquinic acid derivatives, has strong antioxidant capacity, and also has anti-AIDS virus, tumor cell line toxicity, anti-teratogenic, anti-allergic and regulation of enzymes linked by cytochrome P450 Activity and other functions, chlorogenic acid is an important component in many anti-cold virus drugs and other drugs, and it is widely used. Chlorogenic acid is known as "plant gold" and is the target of many manufacturers. Domestic production of chlorogenic acid mainly uses honeysuckle and Eucommia as raw materials, while overseas mainly uses coffee beans as raw materials. The raw materials are inevitably limited and the cost is high. [0003] Compositae plant burdock (Arctium la...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07C67/48C07C69/732
Inventor 刘世名陈靠山李光友
Owner SHANDONG UNIV
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