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Inoculation method for high-effective initiating green smut rice

A high-efficiency technology for rice smut, applied in the agricultural field, can solve the problems of low incidence of rice smut, and achieve the effect of increasing the incidence.

Inactive Publication Date: 2003-12-10
JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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  • Summary
  • Abstract
  • Description
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AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to overcome the problem of low incidence of rice smut by artificial inoculation at present, and propose a method for inducing rice smut with high efficiency by rationally preparing the inoculum, determining the growth period of rice, grasping the inoculation period, and rationally utilizing culture substrates and emulsifiers. The inoculation method of rice false smut, thus laying the foundation for the in-depth study of rice false smut

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Use MHC liquid, wherein the spore content is 200 (per visual field under 10×10 times microscope), inoculate at 16 o’clock in the evening 6 days before rice breach, inoculate rice Yueyou 938, and the incidence rate of rice is 98.28%.

Embodiment 2

[0035] Use thin-walled conidia liquid, wherein the spore content is 190 (per field of view under a 10×10 times microscope), inoculate at 16:00 in the evening 8 days before the rice breach, and inoculate the rice Wuyujing No. 3, and the incidence rate of rice is 98.33%.

Embodiment 3

[0037] Use MHC liquid, wherein the spore content is 200 (per field of view under a 10×10 times microscope), inoculate at 18 o'clock in the evening 9 days before the rice breach, inoculate rice Huai 9805, and the incidence rate of rice is 87.19%.

[0038] It is worth mentioning that the above inoculation process, such as injection inoculation, is better than other inoculation methods.

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PUM

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Abstract

The inoculation method for initiating green smut rice to further develop disease-resisting and breeding research of rice includes the following steps: preparing inoculum body, using clear water to shake and elute and chlamydospore of smut ball surface, shake culture for 5-15 days to obtain spore-hypha fragment suspension, then inoculating the prepared inoculum body on the rice so as to make the disease percentage of green smut be up to above 80%.

Description

technical field [0001] The invention relates to a preparation and inoculation method of strains, in particular to an inoculation method for inducing rice false smut, and belongs to the field of agricultural technology. Background technique [0002] Rice false smut generally occurs in various rice regions in the world, more often in China, Japan and India, and there are also reports of large occurrences in the United States. When the disease is severe, the incidence rate can be as high as 44.1%, and the yield loss can reach 26.9%. In recent years, in some rice areas, especially in China, with the promotion of improved varieties and the improvement of fertilization levels, rice smut has become one of the important diseases of rice production. In particular, the smut fungus can produce toxins that are harmful to humans and animals, and the smut fungus mixed in diseased rice constitutes a direct hazard to people's health and has become a prominent problem in the pollution-free ...

Claims

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Application Information

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IPC IPC(8): A01G22/22A01N63/00C12N1/14
Inventor 陈志谊张君成刘永锋
Owner JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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