Mass production of virus insecticides
A nuclear polyhedron, virus pesticide technology, applied in biocides, animal repellents, plant growth regulators, etc. It is difficult to mass-produce and other problems to achieve the safe and efficient effect of pesticides
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Embodiment 1
[0025] Example 1, 25,000 spring inchworm pupae were packed into moist sandy soil, refrigerated in a refrigerator at 4 degrees, and began to emerge after 88 days, and 2 pairs of adults were placed in each egg-laying container. The artificial feed for spring inchworm larvae was heated and mixed according to formula 1, and then packed into a spring inchworm larvae rearing container. The feed distribution device was placed in a feeding container for spring inchworm larvae, and after natural cooling, a concentration of 1.1 × 10 was placed on the surface. 6 PIB / ml virus, after drying, 12 4th instar larvae were inserted into each worm container, and placed in a replica worm breeding room for rearing at room temperature of 18°C. After 10 days, the virus was harvested and processed into the final product using conventional techniques, with a product content of 1.25×10 10 PIB / ml, with a total production of 70,000ml, control 35,000 mu of 2-3 instar spring inchworm larvae, and the contem...
Embodiment 2
[0026] Example 2, 98,000 spring inchworm pupae were packed into moist sandy soil, refrigerated in a 4°C refrigerator, and began to emerge after 90 days, and 1 pair of adults was placed in each egg-laying container. The artificial feed for spring inchworm larvae was heated and mixed according to formula 2, and then packed into the spring inchworm larvae rearing container. The feed distribution device was placed in a feeding container for spring inchworm larvae, and after natural cooling, a concentration of 5 × 10 was placed on the surface. 6 PIB / ml virus, after drying, 10 4th instar larvae were inserted into each worm container, and placed in a replica worm breeding room for rearing at room temperature of 19°C. The virus was harvested after 9 days and processed into the final product using conventional techniques, with a product content of 1.25×10 10 PIB / ml, with a total production of 375,000ml, to control 150,000 mu of 3-4 instar spring inchworm larvae, and the contemporary p...
Embodiment 3
[0027] Example 3. 23,000 pupa of Spring inchworm were put into moist sandy soil, refrigerated in a refrigerator at 0 degree, and began to emerge after 85 days, and 1 pair of adults was placed in each egg-laying container. The artificial feed for spring inchworm larvae was heated and mixed according to formula 3, and then packed into the spring inchworm larvae rearing container. The feed distribution device was placed in a feeding container for spring inchworm larvae, and after natural cooling, a concentration of 1.1 × 10 was placed on the surface. 6 PIB / ml virus, after drying, 10 4th instar larvae were inserted into each worm container, and placed in the replication worm breeding room for rearing at room temperature of 20°C. The virus was harvested after 9 days and processed into the final product using conventional techniques, with a product content of 1.25×10 10 PIB / ml, with a total production of 64,500ml, to control 32,000 mu of 2-3 instar spring inchworm larvae, and the c...
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