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Fungus biological protein wettable powder agent

A technology of fungal biological protein and wettable powder, applied in the field of biological protein

Inactive Publication Date: 2004-05-05
丰汇华农(北京)生物科技股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The present invention aims to propose a wettable powder of fungal protein, which enables the industrial production of fungal active protein products and solves the stability problem of fungal protein products

Method used

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  • Fungus biological protein wettable powder agent

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Effect test

Embodiment 1

[0039] Embodiment one: the high-efficiency tomato Botrytis cinerea strain (being the fungus Botrytis Botrytis) obtained by screening is cultivated and fermented in PDA culture medium (24-26 ℃) for 3-4 days, and through vacuum suction filtration, the collected by suction filtration Fungal hyphae and spores are added into 5mM phosphate buffer or citric acid buffer at pH 6.8-8.0 according to 1 / 10 of the culture medium volume, and the fungal hyphae and spore suspension is crushed by ultrasonic waves for 20-30 minutes or 1000-1800bar high pressure and spore cells, then centrifuged at 5000rpm for 30 minutes to remove the precipitated broken cell tissue, and the supernatant was bathed in boiling water at 100°C for 15-20 minutes to obtain a special biological protein with a molecular weight between 42-60KD that induces and activates biological activity , and auxiliary active proteins with molecular weights of 30KD and 20KD. 1 gram of microbial special protein that it obtains and 60 gr...

Embodiment 2

[0040] Embodiment two: the high-efficiency vegetable black spot bacterial strain (being Alternaria) obtained by screening is cultured and fermented in PDA culture medium (25-28 ℃) for 2-3 days, and is collected by suction filtration through vacuum filtration. Add 5mM phosphate buffer or citric acid buffer at pH 6.8-8.0 according to 1 / 10 of the volume of the culture medium. The suspension of fungal hyphae and spores is broken by ultrasonic waves for 20-30 minutes or 1000-1800bar high pressure. The silk and spore cells are centrifuged at 5000rpm for 30 minutes to remove the precipitated broken cell tissue, and the supernatant is bathed in 100-degree boiling water for 15-20 minutes to obtain special biological proteins with a molecular weight of 42KD that induce and activate biological activity. And auxiliary active proteins with molecular weights of 30KD and 20KD. 3 grams of microbial special protein that it obtains and 55 grams of diatomaceous earth, 22 grams of tensio-active a...

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Abstract

A wettable fungus protein powder for increasing the resistance of crops to diseases and pests and the yield of crops by 10-30% is prepared from fungus protein, diatomite, surfactant, Na2EDTA, sodium thiosulfate, and phosphoric acid buffering liquid prepared from sodium bihydrogen phosphate and bisodium hydrogen phosphate. Its advantages are high stability and action durability, no poison and no environmental pollution.

Description

Technical field: [0001] The invention relates to biological protein extracted from microorganism fungus, in particular to the dosage form of fungal biological protein product. Background technique: [0002] With the process of global economic integration, with the goal of improving the quality and international competitiveness of my country's agricultural products and ensuring the safety of agricultural production, focusing on solving the key problems that restrict the healthy production of my country's agriculture, research and develop the key to ensure the quality, safety and environmental friendliness of agricultural products Technology and its series of products, establishing an innovation system for agricultural healthy production technology, building a demonstration base for green and clean production of agricultural products, cultivating environmentally friendly agricultural high-tech enterprises, and comprehensively improving the overall level of my country's agricultu...

Claims

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Application Information

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IPC IPC(8): A01N25/14A01N59/26
Inventor 于耀平邱德文
Owner 丰汇华农(北京)生物科技股份有限公司
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