Method of plant breeding under imitated space environment on ground
A technology for plant breeding and space simulation, applied in the fields of botany equipment and methods, plant gene improvement, application, etc., can solve the problems of large investment in space breeding, high technical requirements, limited opportunities, etc., and achieve good application prospects
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Embodiment 1
[0024] Embodiment 1 simulated space environment treatment to wheat seed germination and seedling growth experiment
[0025] 1 Materials and methods
[0026] 1.1 Test materials
[0027] Wheat Triticum aestivum L. Varieties Y1225, Y1221, R344 and Jinghua 1 (JH1).
[0028] 1.2 Test method
[0029] Y1225, Y1221 and R344 each have 200 air-dried seeds of each variety. After 216 days of treatment in the simulated space environment (MF) at room temperature, the indoor germination test was carried out at the same time as the untreated control at a constant temperature of 21°C. Each treatment 100 seeds, two repetitions. The germination potential, germination rate and seedling height and root length after 10 days were investigated and counted.
[0030] The simulated space environment is a large-scale 26-hedron magnetic shielding device with a double-layer magnetic shielding structure and a coil compensation method. Its diameter is 2.3m and its magnetic strength is ≤20nT, which is 4×1...
Embodiment 2
[0046] Example 2 Simulated Space Environment Treatment of Wheat Anther Callus Induction and Differentiation
[0047] Test materials and test methods are the same as in Example 1.
[0048] The results are shown in Table 2.
[0049] Table 2 Effects of simulated space environment treatment on anther callus induction and green shoot differentiation of Jinghua 1 wheat
[0050] Inoculated flowers Healing rate Green seedling rate White seedling rate
[0051] deal with
[0052] Number of healed seedlings Green seedlings White seedlings
[0053] Number of medicines
[0054] (%) (%) (%)
[0055] 1998 MF 4400 258 5.9 69 27.0 - -
[0056] CK 0 2200 72 3.3 6 8.3 - -
[0057] CK 2300 149 6.5 26 18.0 - -
[0058] 1999 MF 4600 361 7.9 31 8.6 28 7.8
[0059] CK 0 2000 93 4.7 12 14.0 13 14.0
[0060] CK 2100 199 9.5 13 6.5 42...
Embodiment 3
[0067] Embodiment 3 The wheat flower cultivation H of simulating space environment treatment 1 Generation, H 2 Research experiments on generational agronomic traits
[0068] Test materials and test methods are the same as in Example 1.
[0069] The results are shown in Table 3 and Table 4.
[0070] Table 3 Effect of simulated space environment treatment on Jinghua No.1 wheat flower cultivation H 1 The effect of generational agronomic traits
[0071] Plant height (cm) Effective number of spikes Spike length (cm) Number of spikelets
[0072] Treatment M±S CV M±S CV M±S CV M±S CV
[0073] % % % %
[0074] MF 82.5±11.5 14.0 8.7±6.4 73.4 7.9±1.0 12.2 21.6±2.0 9.3
[0075] CK 0 86.5±5.0 5.7 9.5±0.7 7.4 7.5±0.7 9.4 23.0±2.8 12.3
[0076] CK 84.3±4.9 5.9 3.5±1.3 36.9 7.5±0.4 5.6 23.0±1.4 6.2
[0077] Seedlings of JH1 88.6±3.9 4.4 7.4±3.1 41.4 8.3±0.3 3.1 19.1±0.9 4.7
[0078] The phenotypic variation coefficients of...
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